FastQCFastQC Report
Sat 14 Jan 2017
SRR2933119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001852
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC231812.313814814962689No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG120681.2045691379565044No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109111.0890830182502005No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT33240.33178553319252746Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT28990.2893640976910761No Hit
GGGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT26340.2629130849666418No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA25570.25522731900520235No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCG25290.2524324950192244No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT24400.24354894734950872No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTAAGTCCTT17470.17437705369655399No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG15700.15670977349947895No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG14700.14672825926384336No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14690.14662844412148698No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG14510.1448317715590726No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG14150.14123842643424378No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC11550.11528648942159123No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC11240.11219222000854417No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCT11170.11149351401204968No Hit
AGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG10860.10839924459900266No Hit
GGGGTTGGGGATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC10730.10710164774837001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAG351.01939804E-746.0000041
AACGCGT351.01939804E-746.00000438
TTACCGA351.01939804E-746.00000413
TACCGAT351.01939804E-746.00000414
ACGCGTC351.01939804E-746.00000439
CGGACAC253.416839E-546.032
ATTAGTT206.311066E-446.044
CGTATTC253.416839E-546.045
CGTATCG206.311066E-446.040
CCGATCA206.311066E-446.036
ACGTTAG206.311066E-446.01
TAGACCG206.311066E-446.01
ACACCGT206.311066E-446.037
CGTTGTC206.311066E-446.043
TAGCGTA253.416839E-546.044
TTCACGC301.8611627E-646.035
ACCGAGT206.311066E-446.027
CACGCAC206.311066E-446.039
GCACGAT206.311066E-446.041
CGATTAC453.092282E-1046.041
Produced by FastQC (version 0.11.5)