##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001852
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46069878584861	33.0	31.0	34.0	31.0	34.0
2	32.833611152146226	34.0	31.0	34.0	31.0	34.0
3	32.87780530457592	34.0	31.0	34.0	31.0	34.0
4	36.32457688361155	37.0	37.0	37.0	35.0	37.0
5	36.18622810554852	37.0	35.0	37.0	35.0	37.0
6	36.296141545857076	37.0	37.0	37.0	35.0	37.0
7	36.49944702411135	37.0	37.0	37.0	35.0	37.0
8	36.423755205359676	37.0	37.0	37.0	35.0	37.0
9	38.26532262250313	39.0	39.0	39.0	37.0	39.0
10	38.00982879706783	39.0	39.0	39.0	35.0	39.0
11	37.67766795894004	39.0	37.0	39.0	35.0	39.0
12	37.19039638589332	39.0	35.0	39.0	35.0	39.0
13	37.06175363227303	39.0	35.0	39.0	34.0	39.0
14	37.82772804765574	40.0	35.0	41.0	33.0	41.0
15	38.10285151898684	40.0	35.0	41.0	33.0	41.0
16	38.29239747986728	40.0	35.0	41.0	34.0	41.0
17	38.348055401396614	40.0	35.0	41.0	35.0	41.0
18	38.300474521186764	40.0	36.0	41.0	35.0	41.0
19	38.254648391179536	40.0	35.0	41.0	35.0	41.0
20	38.09985407026188	40.0	35.0	41.0	34.0	41.0
21	38.03534054930269	40.0	35.0	41.0	34.0	41.0
22	37.966238526249384	40.0	35.0	41.0	34.0	41.0
23	37.9680731285659	40.0	35.0	41.0	34.0	41.0
24	37.94455967548101	40.0	35.0	41.0	34.0	41.0
25	37.76708036716002	40.0	35.0	41.0	34.0	41.0
26	37.688368142200645	39.0	35.0	41.0	34.0	41.0
27	37.58355325936366	39.0	35.0	41.0	34.0	41.0
28	37.56169573949046	39.0	35.0	41.0	34.0	41.0
29	37.50612665343783	39.0	35.0	41.0	34.0	41.0
30	37.43962980560003	39.0	35.0	41.0	34.0	41.0
31	37.281492675564856	39.0	35.0	41.0	33.0	41.0
32	37.16614729520927	39.0	35.0	41.0	33.0	41.0
33	37.05564195110655	39.0	35.0	41.0	33.0	41.0
34	36.875104306823765	39.0	35.0	41.0	33.0	41.0
35	36.810799399512106	39.0	35.0	41.0	32.0	41.0
36	36.72247198188954	39.0	35.0	41.0	32.0	41.0
37	36.60409321935775	39.0	35.0	41.0	32.0	41.0
38	36.628390221310134	39.0	35.0	41.0	32.0	41.0
39	36.60637998426913	39.0	35.0	41.0	32.0	41.0
40	36.50938961044146	39.0	35.0	41.0	32.0	41.0
41	36.33091115254548	39.0	35.0	41.0	31.0	41.0
42	36.38387107077692	39.0	35.0	41.0	31.0	41.0
43	36.353144975505366	38.0	35.0	41.0	32.0	41.0
44	36.31363015694933	38.0	35.0	41.0	31.0	41.0
45	36.25849826122022	38.0	35.0	41.0	31.0	41.0
46	36.22618610333662	38.0	35.0	41.0	31.0	41.0
47	36.12582097954588	38.0	35.0	41.0	31.0	41.0
48	36.00596894551291	38.0	35.0	41.0	31.0	41.0
49	36.005843178433544	38.0	35.0	41.0	31.0	41.0
50	35.90413154837241	37.0	35.0	41.0	31.0	41.0
51	35.829246235970984	37.0	35.0	41.0	31.0	41.0
52	35.567572855072406	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	7.0
15	14.0
16	65.0
17	105.0
18	211.0
19	400.0
20	711.0
21	1129.0
22	1656.0
23	2864.0
24	4707.0
25	6987.0
26	8939.0
27	10106.0
28	10788.0
29	11731.0
30	13844.0
31	16597.0
32	21443.0
33	30858.0
34	79953.0
35	128199.0
36	62546.0
37	73936.0
38	114969.0
39	396706.0
40	2374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.318282540734565	22.502026247389832	24.773419626851073	13.406271585024534
2	39.37966885328372	22.657538239181036	24.80585954811689	13.156933359418357
3	36.05941795794189	22.583874664122046	27.787836926013025	13.568870451923038
4	32.21254237152793	25.50905722601742	27.911408072250193	14.36699233020446
5	26.174924040676668	28.60672035390457	30.493026914154985	14.725328691263778
6	27.54917892063898	35.640294175187556	27.408040309347086	9.402486594826382
7	83.77135544970714	2.2768832122908376	10.550660177351546	3.401101160650475
8	81.10060168567811	2.6037778035079033	11.132183196719675	5.163437314094297
9	74.63487620926045	4.683725739929651	14.314289935040305	6.367108115769595
10	51.634672586370044	16.800685131137133	19.569457364960094	11.995184917532729
11	34.67617971516751	23.99745671017276	25.663870511812124	15.662493062847608
12	33.333566235332164	17.369831072853074	31.908505447910468	17.388097243904287
13	30.281518627501868	19.548096924495834	34.72598747120333	15.444396976798968
14	18.348817989084214	21.687135425192544	41.036200955829806	18.927845629893437
15	15.359554105796066	28.146273102214696	40.04583511337004	16.448337678619197
16	29.63850948044222	23.960425292358554	30.106742313235884	16.29432291396334
17	25.737234641444047	23.514950312022133	26.466184626072515	24.281630420461305
18	22.597549338624866	23.943356903015616	34.47345516104175	18.985638597317767
19	23.523434599122425	25.5504805100953	27.274487648874285	23.651597241907986
20	17.815605498616563	37.979861296878184	25.669360344641724	18.535172859863533
21	24.200380894583233	27.086435920674912	32.9863093550744	15.726873829667456
22	18.673416832027087	22.957782187388958	36.71650104007379	21.652299940510176
23	21.566858178653135	23.140044637331663	34.6039135521015	20.689183631913696
24	23.580229415123192	27.837345236621776	28.863844160614544	19.718581187640492
25	24.12981158893729	23.925789437960894	31.365111812922468	20.579287160179348
26	18.28164239827839	28.952979082738768	27.32689059861137	25.43848792037147
27	23.76897984931906	25.03343807268938	31.472512906097904	19.725069171893654
28	17.335794109309557	30.044657294690236	26.17442496496489	26.445123631035322
29	22.95269161512878	28.366864566822247	26.82022893601051	21.860214882038463
30	26.20536765909536	24.865549003245988	26.67689439158678	22.252188946071875
31	22.396222196492097	26.622095878433143	29.811389307003427	21.170292618071333
32	27.1655893285635	24.78439929251027	25.49687977864994	22.553131600276288
33	25.711582149858465	24.927334576364572	29.839237731720853	19.521845542056113
34	18.172344817398177	24.44363039650567	34.201658528405396	23.182366257690756
35	21.34187484778191	28.49742277302436	28.439130729888245	21.721571649305485
36	25.019364137617135	24.898987075935366	26.681286257850463	23.40036252859704
37	18.577095219653202	29.920087997029498	27.335374885711662	24.167441897605634
38	23.499978040668683	24.172931730435234	29.844128673696314	22.482961555199772
39	19.750322402909813	25.868491553642652	28.734184290693637	25.6470017527539
40	26.98432503004436	20.836910042601104	30.25706391762456	21.92170100972998
41	18.73081053888199	22.97814447642965	27.22497933826553	31.066065646422825
42	17.88577554369308	26.070816847198984	32.86193968769838	23.18146792140955
43	18.782914043192008	26.64834726087286	32.702335275070574	21.866403420864557
44	24.321855922830917	24.174928033282363	28.29060579806199	23.212610245824735
45	19.62196012983954	23.12607051740177	26.573086643536172	30.67888270922252
46	22.75994857523866	22.402011474748765	29.26550029345652	25.57253965655606
47	17.9904816280249	23.526828313962543	32.46896747224141	26.013722585771156
48	18.26597142092844	28.801559511784173	28.66311590933591	24.269353157951475
49	24.911064708160488	23.478218339634996	30.054538993783513	21.556177958421006
50	21.75600787341843	22.781109385418205	29.280772010237037	26.182110730926322
51	19.4800229974088	24.780905762527798	27.471123479316304	28.2679477607471
52	19.878584860837726	25.618055361470553	29.424306184945483	25.079053592746238
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	745.0
1	2058.0
2	3371.0
3	8614.0
4	13857.0
5	8428.5
6	3000.0
7	3490.0
8	3980.0
9	5062.5
10	6145.0
11	7398.5
12	8652.0
13	9017.0
14	8768.5
15	8155.0
16	7480.5
17	6806.0
18	6407.0
19	6008.0
20	5433.5
21	4859.0
22	4779.0
23	4699.0
24	4370.0
25	4041.0
26	5232.5
27	6424.0
28	7519.0
29	8614.0
30	8061.0
31	7508.0
32	8891.0
33	10274.0
34	11557.0
35	12840.0
36	14796.5
37	16753.0
38	18482.5
39	23209.0
40	26206.0
41	33102.0
42	39998.0
43	51535.5
44	63073.0
45	75421.5
46	87770.0
47	92448.0
48	97126.0
49	99992.5
50	102859.0
51	85807.5
52	68756.0
53	65125.5
54	61495.0
55	58770.0
56	56045.0
57	55003.0
58	53961.0
59	61035.0
60	68109.0
61	51022.5
62	33936.0
63	28470.5
64	20724.0
65	18443.0
66	14903.0
67	11363.0
68	9497.0
69	7631.0
70	6837.5
71	6044.0
72	5028.0
73	4012.0
74	3245.0
75	2478.0
76	2010.5
77	1543.0
78	1179.5
79	816.0
80	604.5
81	393.0
82	317.0
83	241.0
84	164.5
85	88.0
86	64.0
87	40.0
88	60.0
89	44.0
90	8.0
91	6.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1001852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.062374669403802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.30834758893484	14.59798384635415
2	12.020090365930809	5.063432936946515
3	5.201393997569242	3.2866112753997414
4	2.778579888197235	2.3409396261632116
5	1.6863606494296222	1.7759379913012912
6	1.0822546605867795	1.3676911889392318
7	0.7875754722000207	1.1611746773136618
8	0.5530300339235467	0.9318500622344665
9	0.4661017130737487	0.883548802332922
>10	4.051168496392943	18.724400078532852
>50	1.3039966477800298	19.914070073109713
>100	0.7420848585988056	20.856380017985206
>500	0.009507813691208272	1.364051469951475
>1k	0.00808164163752703	3.1099932949198275
>5k	0.0	0.0
>10k+	0.0014261720536812407	4.621934658515752
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	23181	2.313814814962689	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	12068	1.2045691379565044	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10911	1.0890830182502005	No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCTT	3324	0.33178553319252746	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	2899	0.2893640976910761	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCT	2634	0.2629130849666418	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2557	0.25522731900520235	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTAAGTCCTTCG	2529	0.2524324950192244	No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGCT	2440	0.24354894734950872	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTAAGTCCTT	1747	0.17437705369655399	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	1570	0.15670977349947895	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCG	1470	0.14672825926384336	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1469	0.14662844412148698	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	1451	0.1448317715590726	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1415	0.14123842643424378	No Hit
CGCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC	1155	0.11528648942159123	No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCC	1124	0.11219222000854417	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCT	1117	0.11149351401204968	No Hit
AGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1086	0.10839924459900266	No Hit
GGGGTTGGGGATCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGC	1073	0.10710164774837001	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.981514235635602E-5	0.0	0.0	0.3420664928552321	0.0
2	9.981514235635602E-5	0.0	0.0	1.0649277537999624	0.0
3	1.9963028471271205E-4	0.0	0.0	1.5939480082886495	0.0
4	1.9963028471271205E-4	0.0	0.0	2.2121031849015624	0.0
5	1.9963028471271205E-4	0.0	0.0	4.103001241700371	0.0
6	1.9963028471271205E-4	0.0	0.0	5.7542431417015685	0.0
7	1.9963028471271205E-4	0.0	0.0	7.218132019499886	0.0
8	1.9963028471271205E-4	0.0	0.0	9.728882110331666	0.0
9	1.9963028471271205E-4	0.0	0.0	11.041850492887173	0.0
10	1.9963028471271205E-4	0.0	0.0	12.323277290458071	0.0
11	1.9963028471271205E-4	0.0	0.0	14.372981238745842	0.0
12	1.9963028471271205E-4	0.0	0.0	15.5563895665228	0.0
13	1.9963028471271205E-4	0.0	0.0	16.061853447415388	0.0
14	1.9963028471271205E-4	0.0	0.0	16.301809049640067	0.0
15	1.9963028471271205E-4	0.0	0.0	16.638984600519837	0.0
16	1.9963028471271205E-4	0.0	0.0	17.24426362376878	0.0
17	1.9963028471271205E-4	0.0	0.0	17.88717295568607	0.0
18	1.9963028471271205E-4	0.0	0.0	18.671220898895246	0.0
19	1.9963028471271205E-4	0.0	0.0	19.111705122113847	0.0
20	1.9963028471271205E-4	0.0	0.0	19.737546064688196	0.0
21	1.9963028471271205E-4	0.0	0.0	20.286828793075223	0.0
22	1.9963028471271205E-4	0.0	0.0	20.88651816835221	0.0
23	4.990757117817802E-4	0.0	0.0	21.37850700502669	0.0
24	4.990757117817802E-4	0.0	0.0	21.73654392065894	0.0
25	4.990757117817802E-4	0.0	0.0	22.172037386759722	0.0
26	4.990757117817802E-4	0.0	0.0	22.496736044844948	0.0
27	4.990757117817802E-4	0.0	0.0	23.049113042645022	0.0
28	4.990757117817802E-4	0.0	0.0	23.472429061378328	0.0
29	4.990757117817802E-4	0.0	0.0	23.99855467673868	0.0
30	4.990757117817802E-4	0.0	0.0	25.583918582784683	0.0
31	4.990757117817802E-4	0.0	0.0	26.098565456774054	0.0
32	4.990757117817802E-4	0.0	0.0	26.422565408862788	0.0
33	4.990757117817802E-4	0.0	0.0	26.889899905375245	0.0
34	4.990757117817802E-4	0.0	0.0	27.367814806977478	0.0
35	4.990757117817802E-4	0.0	0.0	27.824568898400162	0.0
36	4.990757117817802E-4	0.0	0.0	28.134095654847222	0.0
37	4.990757117817802E-4	0.0	0.0	28.552221286178	0.0
38	4.990757117817802E-4	0.0	0.0	29.078546531823065	0.0
39	4.990757117817802E-4	0.0	0.0	30.911252360628115	0.0
40	4.990757117817802E-4	0.0	0.0	31.315902947740785	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAG	35	1.01939804E-7	46.000004	1
AACGCGT	35	1.01939804E-7	46.000004	38
TTACCGA	35	1.01939804E-7	46.000004	13
TACCGAT	35	1.01939804E-7	46.000004	14
ACGCGTC	35	1.01939804E-7	46.000004	39
CGGACAC	25	3.416839E-5	46.0	32
ATTAGTT	20	6.311066E-4	46.0	44
CGTATTC	25	3.416839E-5	46.0	45
CGTATCG	20	6.311066E-4	46.0	40
CCGATCA	20	6.311066E-4	46.0	36
ACGTTAG	20	6.311066E-4	46.0	1
TAGACCG	20	6.311066E-4	46.0	1
ACACCGT	20	6.311066E-4	46.0	37
CGTTGTC	20	6.311066E-4	46.0	43
TAGCGTA	25	3.416839E-5	46.0	44
TTCACGC	30	1.8611627E-6	46.0	35
ACCGAGT	20	6.311066E-4	46.0	27
CACGCAC	20	6.311066E-4	46.0	39
GCACGAT	20	6.311066E-4	46.0	41
CGATTAC	45	3.092282E-10	46.0	41
>>END_MODULE