FastQCFastQC Report
Sat 14 Jan 2017
SRR2933118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599782
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250691.5670260072934938No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC233921.4621992246443578No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG129180.8074850198339524No Hit
GGGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT47910.29947830391890895No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA27980.1748988299655828No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT21470.13420578553827958TruSeq Adapter, Index 20 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC20010.12507954208760944TruSeq Adapter, Index 20 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA19250.12032889481191814No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCG19000.11876618189228282No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC18370.11482814533480187No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT17820.11139017691160422No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT17340.10838976810590444TruSeq Adapter, Index 22 (95% over 23bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG17130.10707708925341078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG551.8189894E-1246.0000041
CGTATCG301.8618648E-646.033
TCGATAG405.6134013E-946.01
GCCGATA301.8618648E-646.09
GTTAACG206.3122E-446.01
AATTGCG405.6134013E-946.01
CGTCGTT206.3122E-446.025
GACGATA301.8618648E-646.09
CGTTTTT75400.044.2002681
ACGGGAT5200.042.9038475
TACGCGG700.042.7142832
CACGACC14150.041.93639427
GGGCGAT8950.041.3743027
CGACCAA14400.041.36805729
CGTAAGG1850.041.0270272
CATACGA3150.040.8888918
ACATACG3150.040.8888917
TAAGGGA15100.040.821194
ACACGAC14600.040.8013726
TAGGGAT16300.040.779145
Produced by FastQC (version 0.11.5)