FastQCFastQC Report
Sat 14 Jan 2017
SRR2933115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889954
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.4302469565842729No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT20980.23574252152358438Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT19650.22079792888171748No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT17170.1929313200457552No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15920.1788856502695645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG551.8189894E-1246.0000041
TGATCGG405.6097633E-946.01
AATCCGT206.310682E-446.011
AATCCGA206.310682E-446.012
CTCACCG206.310682E-446.015
ACCGGTC253.41653E-546.034
TCGCACC206.310682E-446.043
CTCGTCC301.8609262E-646.036
CGAGTGT253.41653E-546.024
CGCGGCC206.310682E-446.013
ACGTTCA206.310682E-446.019
GCGCGTA206.310682E-446.039
CGTGCAC253.41653E-546.044
CGGCCGT206.310682E-446.016
CGGCCCT253.41653E-546.041
GCAACGC206.310682E-446.08
GGCCGTA206.310682E-446.08
GGCGTAA253.41653E-546.07
GACCGGT206.310682E-446.033
TAGCGCA253.41653E-546.016
Produced by FastQC (version 0.11.5)