##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	889954
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.655623773813026	33.0	31.0	34.0	31.0	34.0
2	32.99516042402192	34.0	31.0	34.0	31.0	34.0
3	33.0537263723743	34.0	33.0	34.0	31.0	34.0
4	36.456360665832165	37.0	37.0	37.0	35.0	37.0
5	36.426063594298135	37.0	37.0	37.0	35.0	37.0
6	36.569509210588414	37.0	37.0	37.0	35.0	37.0
7	36.629472983996926	37.0	37.0	37.0	35.0	37.0
8	36.48585769601575	37.0	37.0	37.0	35.0	37.0
9	38.15129770752196	39.0	39.0	39.0	37.0	39.0
10	37.949706389319	39.0	38.0	39.0	35.0	39.0
11	37.31074976908919	39.0	35.0	39.0	35.0	39.0
12	37.07498027987963	39.0	35.0	39.0	34.0	39.0
13	37.11785440595806	39.0	35.0	39.0	35.0	39.0
14	38.44589383271495	40.0	35.0	41.0	35.0	41.0
15	38.520822424529804	40.0	35.0	41.0	35.0	41.0
16	38.5283913550588	40.0	35.0	41.0	35.0	41.0
17	38.56428759239242	40.0	36.0	41.0	35.0	41.0
18	38.50058879447702	40.0	36.0	41.0	35.0	41.0
19	38.388139162248834	40.0	35.0	41.0	35.0	41.0
20	38.27753456920245	40.0	35.0	41.0	35.0	41.0
21	38.222982311445314	40.0	35.0	41.0	35.0	41.0
22	38.19642812999324	40.0	35.0	41.0	35.0	41.0
23	38.16266795811919	40.0	35.0	41.0	35.0	41.0
24	38.129903343318865	40.0	35.0	41.0	35.0	41.0
25	38.08123565937116	40.0	35.0	41.0	35.0	41.0
26	38.01895378862278	40.0	35.0	41.0	35.0	41.0
27	38.03362645709778	40.0	35.0	41.0	35.0	41.0
28	38.07067668666021	40.0	35.0	41.0	35.0	41.0
29	38.05791310562119	40.0	35.0	41.0	35.0	41.0
30	37.952127862788416	40.0	35.0	41.0	35.0	41.0
31	37.87545648426773	40.0	35.0	41.0	35.0	41.0
32	37.78215728003919	40.0	35.0	41.0	35.0	41.0
33	37.65842279488603	40.0	35.0	41.0	34.0	41.0
34	37.55896484537403	40.0	35.0	41.0	34.0	41.0
35	37.451308719327066	40.0	35.0	41.0	34.0	41.0
36	37.35551725145345	40.0	35.0	41.0	34.0	41.0
37	37.27709184969111	40.0	35.0	41.0	33.0	41.0
38	37.33269809450825	39.0	35.0	41.0	34.0	41.0
39	37.2215642606247	39.0	35.0	41.0	33.0	41.0
40	37.29356798216537	39.0	35.0	41.0	34.0	41.0
41	37.27590302420125	39.0	35.0	41.0	34.0	41.0
42	37.21719549549752	39.0	35.0	41.0	34.0	41.0
43	37.12230407414316	39.0	35.0	41.0	34.0	41.0
44	37.05022619146607	39.0	35.0	41.0	34.0	41.0
45	37.012354571135134	39.0	35.0	41.0	34.0	41.0
46	36.95923721900233	39.0	35.0	41.0	33.0	41.0
47	36.90776489571371	39.0	35.0	41.0	33.0	41.0
48	36.90588165231012	39.0	35.0	41.0	34.0	41.0
49	36.808542913453955	39.0	35.0	41.0	33.0	41.0
50	36.703248707236554	38.0	35.0	41.0	33.0	41.0
51	36.59772415203482	38.0	35.0	41.0	33.0	41.0
52	36.41191454839239	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	5.0
15	8.0
16	17.0
17	41.0
18	77.0
19	174.0
20	295.0
21	447.0
22	814.0
23	1336.0
24	2244.0
25	3930.0
26	5479.0
27	6122.0
28	6328.0
29	6940.0
30	8204.0
31	10224.0
32	13824.0
33	19398.0
34	52265.0
35	165240.0
36	40922.0
37	52447.0
38	93513.0
39	396409.0
40	3248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.422134177721542	23.637738579746816	26.973528968913	18.966598273618636
2	24.971852477768515	24.42968962440755	28.750699474354853	21.847758423469077
3	25.684361214175116	25.670652640473552	27.18016886266032	21.464817282691016
4	24.702175618065652	29.358258966193755	24.69857992660295	21.24098548913764
5	22.93174703411637	37.01292426350126	20.463754306402354	19.591574395980015
6	88.72559705333086	3.0359996134631677	6.171442568941766	2.0669607642642203
7	91.497200978927	1.7132346166206343	4.404834407171607	2.3847299972807585
8	84.09254860363569	4.628441470008562	7.488476932515613	3.790532993840131
9	48.15372479925929	30.166840083869506	11.256424489355629	10.423010627515579
10	39.23236481885581	19.948559139011678	27.717949467051106	13.101126575081409
11	36.54076502830483	22.199124898590263	25.064216802216745	16.19589327088816
12	19.182564492097345	38.89796551282426	27.140054429779514	14.779415565298882
13	15.672270701631769	40.523105688608624	28.855199257489712	14.949424352269894
14	14.63525081071606	25.51570081150262	44.16756371677637	15.681484661004951
15	15.669349202318323	21.780563939259782	44.58825961791283	17.96182724050906
16	16.6977169606519	22.028104823395367	28.414389957233745	32.85978825871899
17	19.90136568856368	24.887353728394952	33.92523658526171	21.28604399777966
18	24.965222921634155	27.242082175033765	28.416075437606885	19.376619465725195
19	26.483953103194096	24.488793802825764	27.218373084451557	21.808880009528583
20	19.29234544706805	29.800079554673616	29.941210444584776	20.96636455367356
21	20.62308838434346	24.583180703721766	27.8499787629473	26.94375214898748
22	16.293426401814028	30.970140029709402	25.77357930859348	26.962854259883095
23	16.509280254934524	26.33214750425303	38.91156172116761	18.247010519644835
24	16.66917615966668	26.52833741968686	35.349018039134606	21.453468381511854
25	15.630807884452455	34.53830197965288	28.592938511428677	21.237951624465985
26	15.794973672796573	33.22250363501934	30.64293210660326	20.339590585580826
27	16.270616234097492	29.04296177105783	37.09259130247181	17.593830692372865
28	15.496306550675653	25.28040775141187	35.147884047939556	24.075401649972918
29	18.57994907601966	29.190160390312307	32.40111286650771	19.828777667160324
30	26.685873651896614	26.514741211343505	27.711095180200324	19.088289956559553
31	26.97364133427121	27.164662443227407	27.52007407124413	18.341622151257255
32	25.31580283924787	27.886272773648972	27.084770673540433	19.713153713562722
33	18.682538648064956	26.21371441670019	31.195882034352334	23.907864900882515
34	19.11334743144028	27.383437795661347	30.973398625097477	22.529816147800897
35	28.597320760398855	23.267269993730014	28.38495023338285	19.750459012488285
36	19.270771298291823	31.350272036532225	30.718666358036483	18.66029030713947
37	19.55078577094996	31.278695303352755	26.961168779509954	22.209350146187333
38	19.14098930955982	31.29431408814388	27.730309656454153	21.834386945842144
39	23.148724540818964	26.800149221195703	26.492043408985182	23.55908282900015
40	16.097236486380197	24.966346575216246	31.222962085680834	27.713454852722723
41	20.9550156524944	26.287313726327426	26.00538904257973	26.752281578598446
42	21.93450448000683	25.4506412690993	27.514006341900814	25.100847908993053
43	21.43099530986995	29.290277924476992	28.77171179634004	20.50701496931302
44	18.228919696973104	35.11069111437221	25.055901765709237	21.604487422945457
45	21.404477085332502	29.77659519480782	27.683003840647945	21.135923879211735
46	21.857421844275095	27.34298626670592	27.00218213525643	23.797409753762555
47	21.927537827797842	24.65520689833407	31.92782997772919	21.4894252961389
48	21.08221323798758	23.068158578982732	30.910474024500147	24.93915415852954
49	20.482744051939765	29.76277425574805	28.3891077516366	21.365373940675585
50	18.779959413632614	30.07795908552577	26.97577627607719	24.166305224764425
51	20.275654696759606	24.72105299824485	30.975533566903458	24.027758738092082
52	22.99118830860921	24.402721938437267	30.313364510974722	22.2927252419788
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1748.0
1	1355.5
2	963.0
3	3035.0
4	5107.0
5	3323.5
6	1540.0
7	1738.0
8	1936.0
9	2426.0
10	2916.0
11	2977.0
12	3038.0
13	3061.0
14	3080.0
15	3076.0
16	3069.0
17	3062.0
18	2734.0
19	2406.0
20	2538.5
21	2671.0
22	2897.0
23	3123.0
24	3638.0
25	4153.0
26	4588.5
27	5024.0
28	6667.5
29	8311.0
30	8657.5
31	9004.0
32	11347.0
33	13690.0
34	15791.5
35	17893.0
36	20332.5
37	22772.0
38	25556.5
39	34169.0
40	39997.0
41	53205.0
42	66413.0
43	84103.5
44	101794.0
45	111666.5
46	121539.0
47	114773.5
48	108008.0
49	97086.0
50	86164.0
51	74003.5
52	61843.0
53	53701.5
54	45560.0
55	39994.0
56	34428.0
57	31021.0
58	27614.0
59	23198.0
60	18782.0
61	16169.5
62	13557.0
63	11123.5
64	6731.0
65	4772.0
66	3985.5
67	3199.0
68	2494.0
69	1789.0
70	1439.5
71	1090.0
72	774.5
73	459.0
74	305.5
75	152.0
76	116.5
77	81.0
78	56.5
79	32.0
80	71.5
81	111.0
82	61.0
83	11.0
84	9.0
85	7.0
86	4.5
87	2.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	889954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.785283610748262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84561123655214	15.216064497543542
2	12.586207693469076	5.483882083720111
3	5.024857799768285	3.2840385681489788
4	2.623908644134194	2.286503759246294
5	1.5159249621990742	1.6512427617059833
6	0.9775017081737725	1.277709116553391
7	0.6772533435210861	1.0327909315454067
8	0.49202490134626753	0.85751216155031
9	0.35795562035096273	0.701834823846635
>10	3.7491547916534445	19.23050838316172
>50	1.285519330501911	20.1857448840223
>100	0.8573576226754958	26.941963917166024
>500	0.004136828094936048	0.5883838331633975
>1k	0.0025855175593350295	1.2618202786259267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3829	0.4302469565842729	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCTT	2098	0.23574252152358438	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1965	0.22079792888171748	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1717	0.1929313200457552	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1592	0.1788856502695645	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24181025086689875	0.0
2	0.0	0.0	0.0	0.8117273477056117	0.0
3	0.0	0.0	0.0	1.3951282875294677	0.0
4	0.0	0.0	0.0	1.7743613714866162	0.0
5	0.0	0.0	0.0	3.619063457212395	0.0
6	0.0	0.0	0.0	3.9460466496021143	0.0
7	0.0	0.0	0.0	5.085094285772073	0.0
8	0.0	0.0	0.0	6.608206716302191	0.0
9	0.0	0.0	0.0	7.786245131770856	0.0
10	0.0	0.0	0.0	8.646064852790143	0.0
11	1.1236535820952544E-4	0.0	0.0	9.55397694712311	0.0
12	2.2473071641905087E-4	0.0	0.0	10.225921789216072	0.0
13	2.2473071641905087E-4	0.0	0.0	10.489755650292038	0.0
14	2.2473071641905087E-4	0.0	0.0	10.669652588785487	0.0
15	2.2473071641905087E-4	0.0	0.0	11.304067401236468	0.0
16	2.2473071641905087E-4	0.0	0.0	12.176696773091644	0.0
17	2.2473071641905087E-4	0.0	0.0	13.196075302768458	0.0
18	2.2473071641905087E-4	0.0	0.0	13.749924153383208	0.0
19	2.2473071641905087E-4	0.0	0.0	14.28242358593815	0.0
20	2.2473071641905087E-4	0.0	0.0	14.907961535090578	0.0
21	4.4946143283810174E-4	0.0	0.0	15.569905860302892	0.0
22	4.4946143283810174E-4	0.0	0.0	16.28556082673936	0.0
23	4.4946143283810174E-4	0.0	0.0	16.800418898055405	0.0
24	5.618267910476272E-4	0.0	0.0	17.183697134908098	0.0
25	5.618267910476272E-4	0.0	0.0	17.607876362149057	0.0
26	5.618267910476272E-4	0.0	0.0	17.996885232270433	0.0
27	5.618267910476272E-4	0.0	0.0	18.4070187897352	0.0
28	5.618267910476272E-4	0.0	0.0	18.850075397155358	0.0
29	5.618267910476272E-4	0.0	0.0	19.352460913710146	0.0
30	6.741921492571526E-4	0.0	0.0	19.835631954011106	0.0
31	6.741921492571526E-4	0.0	0.0	20.238686493908673	0.0
32	6.741921492571526E-4	0.0	0.0	20.645336725268947	0.0
33	6.741921492571526E-4	0.0	0.0	21.089067524838363	0.0
34	6.741921492571526E-4	0.0	0.0	21.594599271423018	0.0
35	7.865575074666781E-4	0.0	0.0	21.999226926335517	0.0
36	7.865575074666781E-4	0.0	0.0	22.433968497248173	0.0
37	7.865575074666781E-4	0.0	0.0	22.858934281996596	0.0
38	7.865575074666781E-4	0.0	0.0	23.28255168244651	0.0
39	7.865575074666781E-4	0.0	0.0	23.731563653851772	0.0
40	7.865575074666781E-4	0.0	0.0	24.1624848025853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGG	55	1.8189894E-12	46.000004	1
TGATCGG	40	5.6097633E-9	46.0	1
AATCCGT	20	6.310682E-4	46.0	11
AATCCGA	20	6.310682E-4	46.0	12
CTCACCG	20	6.310682E-4	46.0	15
ACCGGTC	25	3.41653E-5	46.0	34
TCGCACC	20	6.310682E-4	46.0	43
CTCGTCC	30	1.8609262E-6	46.0	36
CGAGTGT	25	3.41653E-5	46.0	24
CGCGGCC	20	6.310682E-4	46.0	13
ACGTTCA	20	6.310682E-4	46.0	19
GCGCGTA	20	6.310682E-4	46.0	39
CGTGCAC	25	3.41653E-5	46.0	44
CGGCCGT	20	6.310682E-4	46.0	16
CGGCCCT	25	3.41653E-5	46.0	41
GCAACGC	20	6.310682E-4	46.0	8
GGCCGTA	20	6.310682E-4	46.0	8
GGCGTAA	25	3.41653E-5	46.0	7
GACCGGT	20	6.310682E-4	46.0	33
TAGCGCA	25	3.41653E-5	46.0	16
>>END_MODULE