FastQCFastQC Report
Sat 14 Jan 2017
SRR2933113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092440
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96240.4599415036990308No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC91900.43920016822465635No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54730.26156066601670774No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC50930.2434000497027394No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGCTT24540.11727934851178529Illumina Single End Adapter 1 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC24020.1147942115425054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA253.418124E-546.01
ACTTACG206.3126476E-446.019
CCCACGT206.3126476E-446.027
ACCTATC253.418124E-546.020
AATCTCG301.8621413E-646.017
CGAATAA206.3126476E-446.024
GCGATAT1000.046.08
ATCGACG206.3126476E-446.014
ACGCGTC206.3126476E-446.043
CGACGGT4600.044.00000427
CTAACGG1550.043.0322571
GCTACGA1950.042.46153610
ACTACGG1600.041.68751
ACGGTCT4950.041.3535429
CACGACG4950.040.88889325
CGCGACA451.589433E-840.888895
TATCGCC451.589433E-840.8888920
GCGTAAT451.589433E-840.8888941
CGCACTT37300.040.63538734
CGTTTTT38800.040.6056671
Produced by FastQC (version 0.11.5)