FastQCFastQC Report
Sat 14 Jan 2017
SRR2933112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005516
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208942.0779380934763845No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49590.49317962120940895No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT34330.3414167452332932Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT27610.2745853870052789No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25870.25728083889266806No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT21570.21451672574081368No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG21360.21242824579618824No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16920.16827181268125022No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC16740.1664816870144284No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC15360.1527573902354612No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC10940.10879985997239228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC551.8189894E-1246.0000045
GTTACGG1050.046.0000041
ACTATCC206.311076E-446.016
AATCCGT206.311076E-446.034
CTCACGG850.046.01
CTCACGA600.046.023
CGAACTC206.311076E-446.012
GTATTCG206.311076E-446.028
CGTATAT206.311076E-446.038
GTACACG206.311076E-446.08
AGTCAAC301.8611681E-646.019
TTAGCGA206.311076E-446.025
GCGCGCA206.311076E-446.044
ACACCGA253.4168483E-546.029
TCGGGTA301.8611681E-646.04
CGACCGT253.4168483E-546.031
CGTCTAC206.311076E-446.017
TACGGCA206.311076E-446.018
CCGTCTA206.311076E-446.016
ACCTAGT206.311076E-446.028
Produced by FastQC (version 0.11.5)