##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933112.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1005516
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.479023705241886	33.0	31.0	34.0	31.0	34.0
2	32.81429335783817	34.0	31.0	34.0	31.0	34.0
3	32.845696140091256	34.0	31.0	34.0	31.0	34.0
4	36.315006424562114	37.0	37.0	37.0	35.0	37.0
5	36.280435119878746	37.0	37.0	37.0	35.0	37.0
6	36.44610428874329	37.0	37.0	37.0	35.0	37.0
7	36.50923903746932	37.0	37.0	37.0	35.0	37.0
8	36.3467811551482	37.0	37.0	37.0	35.0	37.0
9	38.016797345840345	39.0	39.0	39.0	35.0	39.0
10	37.842123844871686	39.0	38.0	39.0	35.0	39.0
11	37.189173518869914	39.0	35.0	39.0	35.0	39.0
12	36.9640522875817	39.0	35.0	39.0	33.0	39.0
13	36.81698650245247	39.0	35.0	39.0	33.0	39.0
14	38.22227592599223	40.0	35.0	41.0	34.0	41.0
15	38.350996901093566	40.0	35.0	41.0	35.0	41.0
16	38.3647758961568	40.0	35.0	41.0	35.0	41.0
17	38.36870621650973	40.0	36.0	41.0	35.0	41.0
18	38.246906066139175	40.0	36.0	41.0	35.0	41.0
19	38.03330926608826	40.0	35.0	41.0	34.0	41.0
20	37.901216887647735	40.0	35.0	41.0	34.0	41.0
21	37.87716754382824	40.0	35.0	41.0	34.0	41.0
22	37.839024938439564	40.0	35.0	41.0	34.0	41.0
23	37.78910728422024	40.0	35.0	41.0	34.0	41.0
24	37.69735041511025	39.0	35.0	41.0	34.0	41.0
25	37.60976453880396	39.0	35.0	41.0	34.0	41.0
26	37.515255848738356	39.0	35.0	41.0	34.0	41.0
27	37.469647424804776	39.0	35.0	41.0	34.0	41.0
28	37.445480728302684	39.0	35.0	41.0	34.0	41.0
29	37.40213681333763	39.0	35.0	41.0	34.0	41.0
30	37.19827133531441	39.0	35.0	41.0	34.0	41.0
31	37.12022483978375	39.0	35.0	41.0	34.0	41.0
32	36.965052768926604	38.0	35.0	41.0	33.0	41.0
33	36.74475194825344	38.0	35.0	41.0	33.0	41.0
34	36.54910513606944	38.0	35.0	41.0	33.0	41.0
35	36.44276371534615	38.0	35.0	41.0	32.0	41.0
36	36.349287331081754	38.0	35.0	41.0	32.0	41.0
37	36.26672673532793	38.0	35.0	41.0	32.0	41.0
38	36.27950723807478	38.0	35.0	41.0	32.0	41.0
39	36.16650157729961	38.0	35.0	41.0	31.0	41.0
40	36.10850647826589	38.0	35.0	41.0	31.0	41.0
41	36.06481448331006	38.0	35.0	41.0	31.0	41.0
42	35.96768425365683	37.0	35.0	41.0	31.0	41.0
43	35.85033853265388	37.0	35.0	41.0	31.0	41.0
44	35.741988193126716	37.0	35.0	41.0	31.0	41.0
45	35.69342407281436	37.0	35.0	41.0	31.0	41.0
46	35.59889449794931	36.0	35.0	40.0	31.0	41.0
47	35.49502345064624	36.0	35.0	40.0	31.0	41.0
48	35.46514426423846	36.0	35.0	40.0	30.0	41.0
49	35.338179601319126	36.0	35.0	40.0	30.0	41.0
50	35.19369855874994	36.0	35.0	40.0	30.0	41.0
51	35.06320038666714	36.0	35.0	40.0	29.0	41.0
52	34.81361609362755	35.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	5.0
14	4.0
15	25.0
16	32.0
17	94.0
18	214.0
19	415.0
20	735.0
21	1196.0
22	1840.0
23	3040.0
24	5038.0
25	7932.0
26	10612.0
27	11721.0
28	11810.0
29	12673.0
30	13844.0
31	17023.0
32	21251.0
33	28775.0
34	72428.0
35	172060.0
36	78338.0
37	72761.0
38	105425.0
39	352652.0
40	3570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.417715879210277	23.22479204706837	24.27927551625235	22.078216557469002
2	28.500690192895984	22.95169843145211	27.354114703296617	21.193496672355288
3	25.149773847457425	24.41144646131936	29.570389730247953	20.86838996097526
4	23.390080316971584	27.741080201607932	27.313041264385653	21.555798217034834
5	21.349436508220656	35.200931660958155	25.43251425138934	18.017117579431854
6	82.16706646139892	3.91828673039514	11.304842488831605	2.609804319374331
7	84.17031653399847	3.239829102669674	8.732730259886466	3.857124103445395
8	76.44532757310675	4.840997060215849	13.396902684790696	5.316772681886714
9	45.634778561455015	22.08696828295124	17.464863811217327	14.813389344376422
10	39.3000210837023	20.812896065303786	25.751355522935487	14.135727328058428
11	34.32735033554911	20.497833947943146	28.298903249674794	16.875912466832947
12	21.845301317930296	32.573822793471216	30.40578170809813	15.17509418050036
13	14.344575322520974	35.46099713977699	34.11959630677185	16.07483123093019
14	13.600977010808382	26.274967280480865	44.609036554366114	15.515019154344634
15	17.31618392944518	21.238448716877702	42.28097812466435	19.164389229012766
16	17.160343545005748	24.2558049797318	29.88236885340462	28.70148262185783
17	20.30161628457429	26.29038225150072	33.60344340617156	19.804558057753432
18	24.967280480867533	30.597424605873996	27.574001806037895	16.861293107220572
19	22.650460062296375	28.858019166278808	27.167543828243407	21.32397694318141
20	18.450029636524928	32.25239578485076	30.001909467377942	19.295665111246365
21	16.538672681488908	28.753097911917862	29.515591994558015	25.19263741203521
22	13.650006563794111	33.826910760246484	27.3091626587742	25.213920017185202
23	15.86061285946718	30.792846657835383	35.50226948153983	17.844271001157612
24	19.40187923414446	27.518806264644226	32.86720449997812	20.212110001233196
25	14.512449329498486	33.15750321228106	28.958862912176436	23.37118454604402
26	15.018557636079386	32.14240250776715	31.26782666809877	21.571213188054692
27	18.359329936072623	29.361939541489146	33.08898117981215	19.189749342626076
28	15.507759200251414	31.128992477494144	30.588573428965827	22.774674893288623
29	22.21715019949956	29.93408359489058	28.869853885965018	18.978912319644838
30	24.9478874528103	29.917574658185448	25.758714928454644	19.37582296054961
31	28.668663651299433	28.977659231678064	23.02390016668059	19.329776950341916
32	25.351361887826744	30.039402654955268	21.996666388202673	22.61256906901531
33	19.258669180798716	29.727423531798596	27.73471531034812	23.279191977054566
34	18.629141654633045	30.401505296782943	26.820856157435585	24.148496891148426
35	26.690077532331657	27.391210085170204	23.969782678744046	21.948929703754093
36	18.01115049387578	35.22181646040441	25.337339236769978	21.429693808949832
37	19.921413483226523	30.796128554891222	26.07367759438935	23.20878036749291
38	16.867757449906318	30.64575799887819	24.686827459732115	27.799657091483375
39	22.745833979767603	27.51791120181081	26.80056806654494	22.93568675187665
40	16.332112069822855	26.270889772017554	29.61564012904817	27.78135802911142
41	19.96795675056389	29.90673445275858	25.230826759594077	24.89448203708345
42	20.103807398390476	26.66730315579265	28.01586449146508	25.2130249543518
43	21.013489591413762	29.076812303334805	29.18372258621444	20.725975519036993
44	17.30653714112953	33.17102860620816	24.75316156083046	24.769272691831855
45	20.704792365312937	28.879401222854735	26.994995604246974	23.42081080758536
46	21.77598367405392	28.482788936227767	26.75114070785547	22.990086681862845
47	19.44872085575963	27.569824846148645	29.87033523086654	23.111119067225186
48	20.09793976426034	24.8056719137239	29.58739592408276	25.508992397933
49	19.24345311263073	28.908142684949816	28.55678079712307	23.291623405296384
50	17.734675529777746	29.596247100990936	28.267377147653544	24.401700221577777
51	17.926616781831417	25.449918250927883	30.235321964046317	26.38814300319438
52	20.320611506927786	24.63501326682022	31.209548132501126	23.83482709375087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5386.0
1	3924.0
2	2462.0
3	13359.0
4	24256.0
5	14173.5
6	4091.0
7	5206.5
8	6322.0
9	8153.5
10	9985.0
11	11541.5
12	13098.0
13	13317.0
14	12291.5
15	11047.0
16	9639.5
17	8232.0
18	7562.5
19	6893.0
20	6254.0
21	5615.0
22	5691.0
23	5767.0
24	5409.0
25	5051.0
26	5274.0
27	5497.0
28	5613.5
29	5730.0
30	6689.5
31	7649.0
32	8543.5
33	9438.0
34	12293.5
35	15149.0
36	15750.5
37	16352.0
38	18753.5
39	22920.0
40	24685.0
41	35266.5
42	45848.0
43	56885.0
44	67922.0
45	73164.0
46	78406.0
47	80824.0
48	83242.0
49	93313.5
50	103385.0
51	105084.5
52	106784.0
53	94401.5
54	82019.0
55	70586.0
56	59153.0
57	51689.5
58	44226.0
59	38739.0
60	33252.0
61	28729.5
62	24207.0
63	20756.5
64	14982.5
65	12659.0
66	9379.5
67	6100.0
68	5260.0
69	4420.0
70	3923.0
71	3426.0
72	2990.0
73	2554.0
74	1918.0
75	1282.0
76	1156.5
77	1031.0
78	787.5
79	544.0
80	369.0
81	194.0
82	138.0
83	82.0
84	74.5
85	67.0
86	35.5
87	4.0
88	3.5
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1005516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.77124820356529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80402176012315	17.78675245034213
2	11.345296680022066	5.620743200078237
3	4.621706800204066	3.434563388158814
4	2.468845745486182	2.446255629510176
5	1.5328481252744748	1.898528068477188
6	1.0289510319089832	1.5293040840439227
7	0.7254495650555804	1.2579203864612187
8	0.5719184028122303	1.1333706166598712
9	0.42738313781692056	0.952813240639334
>10	3.7573644835212106	20.831061949679693
>50	1.2951191971101121	23.106623579773487
>100	0.41021510233210423	14.29013992779645
>500	0.006447388641761951	1.22776958188261
>1k	0.00402961790110122	2.3985427059396125
>5k	0.0	0.0
>10k+	4.0296179011012193E-4	2.0856111905573376
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20894	2.0779380934763845	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4959	0.49317962120940895	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCTT	3433	0.3414167452332932	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	2761	0.2745853870052789	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2587	0.25728083889266806	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	2157	0.21451672574081368	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	2136	0.21242824579618824	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1692	0.16827181268125022	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1674	0.1664816870144284	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1536	0.1527573902354612	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	1094	0.10879985997239228	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.352157499234224	0.0
2	0.0	0.0	0.0	1.004658304790774	0.0
3	0.0	0.0	0.0	1.7995735522855927	0.0
4	0.0	0.0	0.0	2.3401915036657797	0.0
5	0.0	0.0	0.0	5.00270507878542	0.0
6	0.0	0.0	0.0	5.755850727387729	0.0
7	0.0	0.0	0.0	7.681926493462063	0.0
8	0.0	0.0	0.0	10.116696303191596	0.0
9	0.0	0.0	0.0	12.126112364199077	0.0
10	0.0	0.0	0.0	13.494762887910287	0.0
11	0.0	0.0	0.0	14.823831744099547	0.0
12	0.0	0.0	0.0	15.754796542272823	0.0
13	0.0	0.0	0.0	16.13917630350984	0.0
14	0.0	0.0	0.0	16.382335039919802	0.0
15	0.0	0.0	0.0	17.064174016127044	0.0
16	0.0	0.0	0.0	17.975149077687476	0.0
17	0.0	0.0	0.0	18.983387633811894	0.0
18	0.0	0.0	0.0	19.473086455113595	0.0
19	0.0	0.0	0.0	20.05597126251596	0.0
20	0.0	0.0	0.0	20.66003922364239	0.0
21	0.0	0.0	0.0	21.25416204217536	0.0
22	0.0	0.0	0.0	21.86678282593216	0.0
23	0.0	0.0	0.0	22.272445192319168	0.0
24	2.9835427780363515E-4	0.0	0.0	22.631265937091005	0.0
25	2.9835427780363515E-4	0.0	0.0	22.961444671193696	0.0
26	2.9835427780363515E-4	0.0	0.0	23.39147263693467	0.0
27	2.9835427780363515E-4	0.0	0.0	23.77008421546748	0.0
28	2.9835427780363515E-4	0.0	0.0	24.169282239168744	0.0
29	2.9835427780363515E-4	0.0	0.0	24.554159257535435	0.0
30	2.9835427780363515E-4	0.0	0.0	24.936848344531565	0.0
31	2.9835427780363515E-4	0.0	0.0	25.25091594763286	0.0
32	2.9835427780363515E-4	0.0	0.0	25.615604326534832	0.0
33	2.9835427780363515E-4	0.0	0.0	26.005155561920446	0.0
34	3.978057037381802E-4	0.0	0.0	26.47347232664622	0.0
35	3.978057037381802E-4	0.0	0.0	26.81787261465755	0.0
36	3.978057037381802E-4	0.0	0.0	27.200959507357418	0.0
37	3.978057037381802E-4	0.0	0.0	27.62024671909746	0.0
38	3.978057037381802E-4	0.0	0.0	28.49512091304365	0.0
39	3.978057037381802E-4	0.0	0.0	28.88327982846618	0.0
40	4.972571296727252E-4	0.0	0.0	29.254333098627967	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	55	1.8189894E-12	46.000004	5
GTTACGG	105	0.0	46.000004	1
ACTATCC	20	6.311076E-4	46.0	16
AATCCGT	20	6.311076E-4	46.0	34
CTCACGG	85	0.0	46.0	1
CTCACGA	60	0.0	46.0	23
CGAACTC	20	6.311076E-4	46.0	12
GTATTCG	20	6.311076E-4	46.0	28
CGTATAT	20	6.311076E-4	46.0	38
GTACACG	20	6.311076E-4	46.0	8
AGTCAAC	30	1.8611681E-6	46.0	19
TTAGCGA	20	6.311076E-4	46.0	25
GCGCGCA	20	6.311076E-4	46.0	44
ACACCGA	25	3.4168483E-5	46.0	29
TCGGGTA	30	1.8611681E-6	46.0	4
CGACCGT	25	3.4168483E-5	46.0	31
CGTCTAC	20	6.311076E-4	46.0	17
TACGGCA	20	6.311076E-4	46.0	18
CCGTCTA	20	6.311076E-4	46.0	16
ACCTAGT	20	6.311076E-4	46.0	28
>>END_MODULE