FastQCFastQC Report
Sat 14 Jan 2017
SRR2933111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642704
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83261.2954641639074909No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC71241.1084418332544996No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT44050.6853854962782245TruSeq Adapter, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT43780.6811844955064851Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT41860.6513107122407826TruSeq Adapter, Index 23 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG35090.5459745077049466No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT16440.25579426921257686TruSeq Adapter, Index 23 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC15500.24116856282207672Illumina Single End Adapter 1 (95% over 21bp)
GGGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT12560.19542433219646993TruSeq Adapter, Index 23 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT12310.19153451666708157Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC12110.1884226642435709Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG11270.1753528840648261No Hit
ACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC10690.16632851203664517Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCG9860.1534143244790759No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA8340.12976424606039483No Hit
GGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC8270.12867509771216612TruSeq Adapter, Index 23 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC7770.12089546665338942TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTTCTCTGCT7590.11809479947222984No Hit
TGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC7260.11296024297343722Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA351.0186341E-746.00000421
TTTAGTC206.3093664E-446.00000431
GTTGACA206.3093664E-446.00000439
TTGATCG351.0186341E-746.00000420
CTTAAAC206.3093664E-446.00000434
ACAACCG206.3093664E-446.00000418
ACAACCC206.3093664E-446.00000446
CTATGTA206.3093664E-446.00000417
CGTGGTA206.3093664E-446.00000439
AGACTTA206.3093664E-446.00000439
CTAGAGT206.3093664E-446.00000418
CGGGTGA405.6043064E-946.0000046
TTCTAAC206.3093664E-446.00000445
AACCGTC206.3093664E-446.00000420
AACCGAT206.3093664E-446.00000424
AATCCGA206.3093664E-446.00000428
GCCCATT206.3093664E-446.00000427
CCCCTCG206.3093664E-446.00000434
TACCCGG206.3093664E-446.00000444
TCGCAAG405.6043064E-946.0000041
Produced by FastQC (version 0.11.5)