##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642704
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33599915357614	33.0	31.0	34.0	31.0	34.0
2	32.69065697428365	34.0	31.0	34.0	31.0	34.0
3	32.72877561054545	34.0	31.0	34.0	31.0	34.0
4	36.21152816848814	37.0	37.0	37.0	35.0	37.0
5	36.079213759366674	37.0	35.0	37.0	35.0	37.0
6	36.190443812392644	37.0	36.0	37.0	35.0	37.0
7	36.44707361398093	37.0	37.0	37.0	35.0	37.0
8	36.33135160198163	37.0	37.0	37.0	35.0	37.0
9	38.17144595334711	39.0	39.0	39.0	37.0	39.0
10	37.93864360576564	39.0	38.0	39.0	35.0	39.0
11	37.63315460927581	39.0	37.0	39.0	35.0	39.0
12	37.16726362368991	39.0	35.0	39.0	35.0	39.0
13	37.04263549005452	39.0	35.0	39.0	33.0	39.0
14	37.87724208967114	40.0	36.0	41.0	33.0	41.0
15	38.149840361970675	40.0	36.0	41.0	33.0	41.0
16	38.381013654808434	40.0	36.0	41.0	34.0	41.0
17	38.40239363688416	40.0	36.0	41.0	35.0	41.0
18	38.40055453210187	40.0	36.0	41.0	35.0	41.0
19	38.35386585426573	40.0	36.0	41.0	35.0	41.0
20	38.21425881898977	40.0	35.0	41.0	34.0	41.0
21	38.14761227563544	40.0	35.0	41.0	34.0	41.0
22	38.09121617416416	40.0	35.0	41.0	34.0	41.0
23	38.07997771913665	40.0	35.0	41.0	34.0	41.0
24	37.9929189798103	40.0	35.0	41.0	34.0	41.0
25	37.857676317558315	40.0	35.0	41.0	34.0	41.0
26	37.794756217481144	40.0	35.0	41.0	34.0	41.0
27	37.708937551345564	40.0	35.0	41.0	34.0	41.0
28	37.68973119818766	40.0	35.0	41.0	34.0	41.0
29	37.678114653090695	40.0	35.0	41.0	34.0	41.0
30	37.66560189449576	40.0	35.0	41.0	34.0	41.0
31	37.537595845054646	40.0	35.0	41.0	34.0	41.0
32	37.49709041798402	40.0	35.0	41.0	34.0	41.0
33	37.42987284970997	40.0	35.0	41.0	34.0	41.0
34	37.312230824765365	40.0	35.0	41.0	33.0	41.0
35	37.264530794891584	40.0	35.0	41.0	33.0	41.0
36	37.20370497149543	40.0	35.0	41.0	33.0	41.0
37	37.06074491523314	39.0	35.0	41.0	33.0	41.0
38	37.0680593243546	39.0	35.0	41.0	33.0	41.0
39	37.04944266723095	39.0	35.0	41.0	33.0	41.0
40	36.93053256242376	39.0	35.0	41.0	33.0	41.0
41	36.81410727177674	39.0	35.0	41.0	33.0	41.0
42	36.83394999875526	39.0	35.0	41.0	33.0	41.0
43	36.742469317135104	39.0	35.0	41.0	33.0	41.0
44	36.696933580621874	39.0	35.0	41.0	33.0	41.0
45	36.63598795090741	39.0	35.0	41.0	32.0	41.0
46	36.574213012522094	39.0	35.0	41.0	32.0	41.0
47	36.47586291667704	38.0	35.0	41.0	32.0	41.0
48	36.41529382110583	38.0	35.0	41.0	32.0	41.0
49	36.422407515745974	38.0	35.0	41.0	32.0	41.0
50	36.27462253230103	38.0	35.0	41.0	31.0	41.0
51	36.20530757549354	38.0	35.0	41.0	31.0	41.0
52	35.95783284373522	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	2.0
14	3.0
15	6.0
16	33.0
17	68.0
18	114.0
19	198.0
20	327.0
21	548.0
22	827.0
23	1334.0
24	2213.0
25	3305.0
26	4315.0
27	5300.0
28	5839.0
29	6863.0
30	8386.0
31	10660.0
32	13546.0
33	19075.0
34	44722.0
35	88497.0
36	46530.0
37	46881.0
38	66724.0
39	261868.0
40	4516.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.81928850606189	24.596392740670666	25.814682964475093	13.769635788792353
2	34.097811745375786	23.584885110408525	27.229019890960693	15.088283253254996
3	30.789912619183944	24.98957529438124	30.684887599890466	13.53562448654435
4	29.526500535238615	25.807214518658668	29.43485648136623	15.231428464736489
5	23.528093803679454	30.29901789937514	30.1199307924021	16.052957504543304
6	24.52637606114168	36.89770096342951	27.21548333291842	11.360439642510393
7	81.75349772212402	1.7336129851377928	11.391713759366676	5.121175533371505
8	76.32284846523439	1.9601558415693692	12.98638253379472	8.730613159401528
9	70.07331524309791	3.612393885832358	17.47678558092061	8.83750529014912
10	47.65055142024945	17.74222659264607	20.762746147526702	13.844475839577782
11	31.264625706390497	20.307170952724736	29.418363663521625	19.009839677363143
12	29.264326968557842	18.514432771540243	32.74820134929921	19.473038910602703
13	24.409992780502378	24.47051831013966	35.953564938136374	15.16592397122159
14	14.092490477731584	29.51529786651398	36.786452239289005	19.605759416465435
15	10.640979362194727	30.365300355995917	41.03055216709402	17.963168114715327
16	19.496688989021386	27.29623590330852	36.246079065946375	16.960996041723718
17	16.107103736712393	29.198978067664115	27.130218576514224	27.56369961910926
18	16.729163036172174	28.014918220518307	35.64471358510294	19.611205158206577
19	17.168867783614232	29.70729916104459	28.59839054992656	24.525442505414624
20	15.182105603823844	37.19612761084418	26.46552689885235	21.156239886479625
21	18.03271801638079	31.608952177051957	33.09315019044537	17.265179616121884
22	16.164517413926163	27.728316612312977	30.209552142199207	25.89761383156165
23	16.94559237222734	28.391607956384274	29.373397396001895	25.28940227538649
24	19.395242600014935	29.31878438596928	30.83783514650601	20.44813786750977
25	18.787342229082128	26.31849187184147	30.20395080783689	24.690215091239512
26	15.518963628668875	30.59090965670044	28.863987154273197	25.026139560357493
27	21.25006223704847	27.311483980183727	30.413845253802684	21.02460852896512
28	14.690899698772686	30.006036993701613	28.5224613507929	26.780601956732802
29	17.578387562548233	24.592969703004805	31.130816052179544	26.697826682267422
30	21.983525853269935	24.873036421120766	29.70325375289402	23.44018397271528
31	18.907615325250816	25.31383031691105	30.75210361223829	25.026450745599842
32	22.24352112325425	21.8273108616097	30.99389454554507	24.935273469590978
33	20.67841494684956	22.200266374567455	31.770457317832157	25.350861360750827
34	15.05514202494461	20.053088202345094	35.52910826756952	29.36266150514078
35	17.296920510841694	22.592048594687444	35.128612860663694	24.982418033807168
36	23.280701536010355	21.139435883392665	29.323140979362194	26.256721601234783
37	17.592546491075208	23.526071099604174	33.81416639697279	25.06721601234783
38	21.30623117329284	22.95006721601235	30.452899001717743	25.29080260897707
39	19.500578804550774	23.6581692349822	28.243172595782816	28.59807936468421
40	23.42680300729418	20.03924045906047	33.98843013268939	22.54552640095596
41	19.135558515272972	22.770824516418134	28.074976972292063	30.018639996016827
42	19.709072916925987	23.039999751051806	31.566786576713383	25.68414075530882
43	19.62536408673355	22.97885185092982	33.92323682441684	23.472547237919787
44	20.782350817794818	24.5917249620354	27.668413453160394	26.957510767009385
45	18.36957604122582	23.65147875227165	28.790236251835992	29.18870895466653
46	24.843473823097415	21.404565709875776	30.045713112101375	23.706247354925438
47	18.40380641788444	22.599828225746222	32.8386006622022	26.157764694167145
48	17.329750553909733	24.100363464363067	30.94923946326769	27.62064651845951
49	25.037031043839775	19.43414075530882	31.250311185242353	24.27851701560905
50	20.4931041350295	20.069114242326172	32.01100351016953	27.426778112474793
51	17.510860364958052	21.65693694142249	27.902269162787224	32.92993353083224
52	18.241212128756008	22.593137743035673	34.15724812666484	25.008402001543477
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	618.0
1	1209.5
2	1801.0
3	5608.5
4	9416.0
5	5349.5
6	1283.0
7	1713.5
8	2144.0
9	3193.5
10	4243.0
11	5692.5
12	7142.0
13	7309.0
14	6923.0
15	6370.0
16	5547.5
17	4725.0
18	4029.5
19	3334.0
20	3207.5
21	3081.0
22	2958.5
23	2836.0
24	2569.0
25	2302.0
26	2556.5
27	2811.0
28	3333.5
29	3856.0
30	3939.5
31	4023.0
32	3765.5
33	3508.0
34	5272.5
35	7037.0
36	7840.0
37	8643.0
38	10330.5
39	14982.5
40	17947.0
41	22346.5
42	26746.0
43	32513.5
44	38281.0
45	50884.5
46	63488.0
47	76702.0
48	89916.0
49	93242.5
50	96569.0
51	78076.5
52	59584.0
53	49721.0
54	39858.0
55	34687.0
56	29516.0
57	25548.5
58	21581.0
59	23128.5
60	24676.0
61	17505.5
62	10335.0
63	9323.0
64	7335.5
65	6360.0
66	5060.5
67	3761.0
68	3140.0
69	2519.0
70	2129.0
71	1739.0
72	1506.0
73	1273.0
74	928.5
75	584.0
76	581.5
77	579.0
78	402.0
79	225.0
80	197.5
81	170.0
82	91.5
83	13.0
84	7.5
85	2.0
86	1.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	642704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.624313100325793
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.46466973042222	12.650748626989007
2	12.652753709587028	4.96603200756489
3	6.098125527693613	3.5901457404164674
4	3.77762739218013	2.965333708820404
5	2.417483124463821	2.3720722874615947
6	1.6684940191676618	1.964582942290031
7	1.1842620131120816	1.6268229976693533
8	0.8833003184650727	1.386732960894086
9	0.6118541509013478	1.0806495686119832
>10	4.424155242635814	17.640931223736047
>50	0.8197575715399477	11.657676448566422
>100	0.9776779556023383	30.410842355027007
>500	0.009522837229892904	1.2999001471630431
>1k	0.008729267460735164	3.9814600649778797
>5k	0.0015871395383154844	2.4060689198117906
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8326	1.2954641639074909	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	7124	1.1084418332544996	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	4405	0.6853854962782245	TruSeq Adapter, Index 23 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT	4378	0.6811844955064851	Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	4186	0.6513107122407826	TruSeq Adapter, Index 23 (96% over 25bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3509	0.5459745077049466	No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	1644	0.25579426921257686	TruSeq Adapter, Index 23 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1550	0.24116856282207672	Illumina Single End Adapter 1 (95% over 21bp)
GGGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	1256	0.19542433219646993	TruSeq Adapter, Index 23 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	1231	0.19153451666708157	Illumina Single End Adapter 1 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1211	0.1884226642435709	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG	1127	0.1753528840648261	No Hit
ACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	1069	0.16632851203664517	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCG	986	0.1534143244790759	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	834	0.12976424606039483	No Hit
GGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	827	0.12867509771216612	TruSeq Adapter, Index 23 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	777	0.12089546665338942	TruSeq Adapter, Index 23 (95% over 24bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTTCTCTGCT	759	0.11809479947222984	No Hit
TGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	726	0.11296024297343722	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6981440912146183	0.0
2	0.0	0.0	0.0	2.528068908860066	0.0
3	0.0	0.0	0.0	3.8580746346685255	0.0
4	0.0	0.0	0.0	5.307419900918619	0.0
5	0.0	0.0	0.0	9.148379347257835	0.0
6	0.0	0.0	0.0	12.187103238816002	0.0
7	0.0	0.0	0.0	14.5928763474321	0.0
8	0.0	0.0	0.0	19.290217580721453	0.0
9	0.0	0.0	0.0	21.377025815927706	0.0
10	0.0	0.0	0.0	23.65910279070925	0.0
11	0.0	0.0	0.0	27.376366103213922	0.0
12	0.0	0.0	0.0	29.232119295974506	0.0
13	0.0	0.0	0.0	30.0023027707934	0.0
14	0.0	0.0	0.0	30.284547785605817	0.0
15	0.0	0.0	0.0	30.767662874355846	0.0
16	0.0	0.0	0.0	31.716311085663072	0.0
17	1.5559262117553337E-4	0.0	0.0	32.838289476959844	0.0
18	1.5559262117553337E-4	0.0	0.0	33.88698374368294	0.0
19	3.1118524235106674E-4	0.0	0.0	34.51215489556623	0.0
20	3.1118524235106674E-4	0.0	0.0	35.18089198137867	0.0
21	7.779631058776669E-4	0.0	0.0	35.83655928701237	0.0
22	7.779631058776669E-4	0.0	0.0	36.471065996166196	0.0
23	7.779631058776669E-4	0.0	0.0	37.07118673604023	0.0
24	9.335557270532003E-4	0.0	0.0	37.48568547885185	0.0
25	9.335557270532003E-4	0.0	0.0	37.916988224750426	0.0
26	9.335557270532003E-4	0.0	0.0	38.2955450720705	0.0
27	9.335557270532003E-4	0.0	0.0	38.77445916004879	0.0
28	9.335557270532003E-4	0.0	0.0	39.13854589359954	0.0
29	9.335557270532003E-4	0.0	0.0	39.57031541736165	0.0
30	9.335557270532003E-4	0.0	0.0	40.54276929970873	0.0
31	9.335557270532003E-4	0.0	0.0	40.99243197490602	0.0
32	9.335557270532003E-4	0.0	0.0	41.307973810650005	0.0
33	9.335557270532003E-4	0.0	0.0	41.70022280863353	0.0
34	9.335557270532003E-4	0.0	0.0	42.0781572854689	0.0
35	9.335557270532003E-4	0.0	0.0	42.48487639722174	0.0
36	9.335557270532003E-4	0.0	0.0	42.79435012073987	0.0
37	9.335557270532003E-4	0.0	0.0	43.154702631382406	0.0
38	9.335557270532003E-4	0.0	0.0	43.560954965271726	0.0
39	9.335557270532003E-4	0.0	0.0	44.628942717020585	0.0
40	9.335557270532003E-4	0.0	0.0	44.981982374467876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	35	1.0186341E-7	46.000004	21
TTTAGTC	20	6.3093664E-4	46.000004	31
GTTGACA	20	6.3093664E-4	46.000004	39
TTGATCG	35	1.0186341E-7	46.000004	20
CTTAAAC	20	6.3093664E-4	46.000004	34
ACAACCG	20	6.3093664E-4	46.000004	18
ACAACCC	20	6.3093664E-4	46.000004	46
CTATGTA	20	6.3093664E-4	46.000004	17
CGTGGTA	20	6.3093664E-4	46.000004	39
AGACTTA	20	6.3093664E-4	46.000004	39
CTAGAGT	20	6.3093664E-4	46.000004	18
CGGGTGA	40	5.6043064E-9	46.000004	6
TTCTAAC	20	6.3093664E-4	46.000004	45
AACCGTC	20	6.3093664E-4	46.000004	20
AACCGAT	20	6.3093664E-4	46.000004	24
AATCCGA	20	6.3093664E-4	46.000004	28
GCCCATT	20	6.3093664E-4	46.000004	27
CCCCTCG	20	6.3093664E-4	46.000004	34
TACCCGG	20	6.3093664E-4	46.000004	44
TCGCAAG	40	5.6043064E-9	46.000004	1
>>END_MODULE