FastQCFastQC Report
Sat 14 Jan 2017
SRR2933110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762541
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84691.1106288055330795No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC79071.0369278504368946No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG38020.4985961410599561No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT30100.39473287337992313No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT27910.36601310618052013Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT23280.3052950595443393No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT12610.16536815725318377No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGGGAATATTCG9340.12248521718832169No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCG9240.12117381229337176No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA8970.117633019077007No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC8950.11737073809801703No Hit
GGGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT8710.11422336635013725No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8280.10858432530185262No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT7950.1042566891485179No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGA1100.046.0000044
TACGCGG551.8189894E-1246.0000042
CTTGCGG551.8189894E-1246.0000042
CGCTAGG551.8189894E-1246.0000042
TCATCGA1150.046.00000416
TCACGAG453.092282E-1046.01
TTCTACG253.4160657E-546.01
CGGCGTT206.3101115E-446.013
TCGTTGC301.8605715E-646.042
CTCACGA253.4160657E-546.027
CGAACGC206.3101115E-446.021
ACACGAT405.6079443E-946.023
GTCGATT253.4160657E-546.014
GGTCGTA206.3101115E-446.018
GTCGACC253.4160657E-546.044
CAAGTCG206.3101115E-446.01
CGCGGTA206.3101115E-446.031
TTTACGT501.6370905E-1146.016
TTTACGC206.3101115E-446.023
CACGGCA405.6079443E-946.037
Produced by FastQC (version 0.11.5)