##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762541
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41297976108826	33.0	31.0	34.0	31.0	34.0
2	32.774814731273466	34.0	31.0	34.0	31.0	34.0
3	32.82396880954598	34.0	31.0	34.0	31.0	34.0
4	36.28643181153538	37.0	37.0	37.0	35.0	37.0
5	36.17787895995101	37.0	37.0	37.0	35.0	37.0
6	36.2753373261241	37.0	37.0	37.0	35.0	37.0
7	36.49328888545009	37.0	37.0	37.0	35.0	37.0
8	36.414719995383855	37.0	37.0	37.0	35.0	37.0
9	38.253498500408504	39.0	39.0	39.0	37.0	39.0
10	37.94115463955381	39.0	38.0	39.0	35.0	39.0
11	37.68620179111681	39.0	37.0	39.0	35.0	39.0
12	37.33207394749922	39.0	37.0	39.0	35.0	39.0
13	37.25455549275383	39.0	37.0	39.0	34.0	39.0
14	38.184923827046674	40.0	37.0	41.0	33.0	41.0
15	38.41159098330451	40.0	37.0	41.0	33.0	41.0
16	38.597474758734286	40.0	37.0	41.0	35.0	41.0
17	38.622660289741795	40.0	37.0	41.0	35.0	41.0
18	38.6088970953693	40.0	37.0	41.0	35.0	41.0
19	38.576248359104625	40.0	37.0	41.0	35.0	41.0
20	38.442471945770784	40.0	36.0	41.0	34.0	41.0
21	38.37497000161303	40.0	36.0	41.0	34.0	41.0
22	38.334006958314376	40.0	35.0	41.0	34.0	41.0
23	38.309357791908894	40.0	35.0	41.0	34.0	41.0
24	38.26380483147791	40.0	35.0	41.0	34.0	41.0
25	38.15090336126189	40.0	35.0	41.0	34.0	41.0
26	38.03429586081273	40.0	35.0	41.0	34.0	41.0
27	37.95482865839345	40.0	35.0	41.0	34.0	41.0
28	37.94731693115518	40.0	35.0	41.0	34.0	41.0
29	37.901774461963356	40.0	35.0	41.0	34.0	41.0
30	37.85337313010054	40.0	35.0	41.0	34.0	41.0
31	37.777058545048725	40.0	35.0	41.0	34.0	41.0
32	37.678560234793935	40.0	35.0	41.0	34.0	41.0
33	37.580895978052325	40.0	35.0	41.0	34.0	41.0
34	37.48319893618835	40.0	35.0	41.0	33.0	41.0
35	37.405089037835346	40.0	35.0	41.0	33.0	41.0
36	37.33195854386846	40.0	35.0	41.0	33.0	41.0
37	37.2294801197575	40.0	35.0	41.0	33.0	41.0
38	37.16327384363595	40.0	35.0	41.0	33.0	41.0
39	37.1417077901385	40.0	35.0	41.0	33.0	41.0
40	37.07815579752433	40.0	35.0	41.0	33.0	41.0
41	36.975360013428784	40.0	35.0	41.0	32.0	41.0
42	36.98835341312795	40.0	35.0	41.0	32.0	41.0
43	36.9279920686232	39.0	35.0	41.0	32.0	41.0
44	36.865843279246626	39.0	35.0	41.0	32.0	41.0
45	36.78137962417758	39.0	35.0	41.0	32.0	41.0
46	36.68657423010697	39.0	35.0	41.0	32.0	41.0
47	36.54097681305005	39.0	35.0	41.0	32.0	41.0
48	36.39228185763126	39.0	35.0	41.0	32.0	41.0
49	36.29809675807596	38.0	35.0	40.0	31.0	41.0
50	36.104344553276476	38.0	35.0	40.0	31.0	41.0
51	35.988161948013285	37.0	35.0	40.0	31.0	41.0
52	35.71229088009694	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	3.0
15	12.0
16	25.0
17	66.0
18	111.0
19	261.0
20	422.0
21	727.0
22	1142.0
23	1722.0
24	2685.0
25	4118.0
26	5502.0
27	6505.0
28	7431.0
29	8394.0
30	9927.0
31	12106.0
32	15373.0
33	20835.0
34	49369.0
35	83481.0
36	47257.0
37	53416.0
38	93888.0
39	336006.0
40	1754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.14037540276523	23.038498913501044	26.725120354184234	12.096005329549493
2	36.24106769340927	22.82106798191835	26.943993831151374	13.993870493521005
3	31.23556634987496	24.42544073040007	29.377567894709923	14.961425025015048
4	28.132913508912967	28.11665208821558	28.443191907058115	15.30724249581334
5	24.439997324734016	31.53692719473445	28.517679705091265	15.505395775440272
6	26.4772648290387	38.49917578202353	24.871711816151524	10.15184757278625
7	85.97832772270606	1.866653727471703	8.89775107174565	3.2572674780765887
8	82.35504713844894	2.428197303489255	9.783605078284314	5.43315047977748
9	76.397465841181	4.294588749981968	12.814130650024063	6.493814758812968
10	43.36488136375618	24.895185963771127	19.308601111284506	12.431331561188186
11	29.672371715094663	23.474147619603407	29.180726019977943	17.67275464532399
12	29.324062574995967	19.366565207641294	31.74058837492017	19.56878384244257
13	26.895734131017218	23.81012955368957	34.12655844079204	15.167577874501173
14	17.50725534758131	26.732333081106457	35.635592053410896	20.124819517901333
15	14.397783201165575	29.008276276292026	38.51648632663686	18.07745419590553
16	23.262618010047984	26.797378763895974	33.34128918969603	16.59871403636001
17	21.015656863040807	26.451954714566167	27.994822573474735	24.537565848918288
18	18.67099605135986	25.136353323952417	36.30113003759798	19.891520587089744
19	19.427807816235454	28.211466662120465	29.92219434758262	22.43853117406146
20	17.607708962534474	34.90841803916117	27.83692942412277	19.646943574181584
21	20.482046211285688	30.127822635110768	32.46631984378545	16.923811309818095
22	18.90403270119246	26.05014025475351	31.53535350886051	23.51047353519352
23	18.023555454723088	26.659943530905224	32.0409000958637	23.275600918507987
24	20.020431688263322	28.00046161452302	31.22966502784768	20.749441669365975
25	21.375637506704557	26.66217291922664	29.605227784473225	22.356961789595577
26	18.17908807526415	30.31627151851507	27.692543745188786	23.812096661031998
27	23.917402474096473	26.944256112130365	28.865464283231983	20.27287713054118
28	18.60870431884974	29.84206750850118	27.033431644986962	24.515796527662122
29	21.94032845446999	28.279659716657857	26.73154623816949	23.048465590702662
30	27.224109916712674	27.330071432224628	25.43364881363756	20.012169837425134
31	24.67355853652459	28.29408517050231	27.192636199233878	19.83972009373922
32	27.692674885678276	28.319001863506355	23.934188456751833	20.054134794063533
33	26.666500555379972	28.65642634297697	25.917557219874077	18.75951588176898
34	22.439186876508934	27.139524300988406	30.101069975253793	20.32021884724887
35	22.21598576338846	31.789372637012303	26.446577954496874	19.548063645102363
36	26.157413175160414	29.26544277619171	26.186264082849313	18.39087996579856
37	20.756654396288198	30.24086573705545	30.592584529881016	18.409895336775335
38	21.083587636599212	31.37509983069763	28.368966390003948	19.17234614269921
39	19.522491249650837	30.54983273030565	29.24708310766241	20.680592912381105
40	23.22052191292009	27.026218918064732	31.05983809395167	18.693421075063505
41	19.28499582317541	27.42291889878708	28.297101401760692	24.994983876276816
42	20.828519384531454	26.583226344550653	31.714753698489655	20.873500572428235
43	21.387440150759108	26.16200309229274	31.45548895075806	20.995067806190093
44	23.1156095213241	26.807870003055573	26.740857212923636	23.33566326269669
45	20.96897084878059	27.292434111739567	25.937883995745803	25.800711043734044
46	24.449701720956643	26.17931363690608	28.070884057381836	21.300100584755445
47	19.820573582272953	26.648140886850673	30.782344818180267	22.748940712696104
48	18.744303584987563	28.290675517775437	29.518150499448552	23.446870397788448
49	24.246565102728905	23.55152050840545	31.376673516571568	20.825240872294078
50	20.853042656067018	24.98042728194287	30.349187781378312	23.817342280611797
51	20.136228740487397	24.92521713586548	27.26358320405067	27.674970919596454
52	19.45469161658193	25.955194540359138	31.830157329245246	22.759956513813684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	589.0
1	1243.5
2	1898.0
3	5868.0
4	9838.0
5	5643.0
6	1448.0
7	2005.0
8	2562.0
9	3300.5
10	4039.0
11	4987.5
12	5936.0
13	6229.0
14	6190.5
15	5859.0
16	5343.5
17	4828.0
18	4411.5
19	3995.0
20	3749.0
21	3503.0
22	3557.5
23	3612.0
24	3638.5
25	3665.0
26	4554.0
27	5443.0
28	6491.0
29	7539.0
30	8507.0
31	9475.0
32	10230.0
33	10985.0
34	13274.0
35	15563.0
36	16586.0
37	17609.0
38	19176.0
39	24333.5
40	27924.0
41	34620.5
42	41317.0
43	51059.0
44	60801.0
45	72280.0
46	83759.0
47	85191.5
48	86624.0
49	80286.0
50	73948.0
51	63887.0
52	53826.0
53	48122.0
54	42418.0
55	37839.0
56	33260.0
57	32025.0
58	30790.0
59	31570.5
60	32351.0
61	25708.0
62	19065.0
63	15169.5
64	9462.5
65	7651.0
66	6000.5
67	4350.0
68	3344.0
69	2338.0
70	2226.5
71	2115.0
72	1542.0
73	969.0
74	897.0
75	825.0
76	686.0
77	547.0
78	430.5
79	314.0
80	283.5
81	253.0
82	195.5
83	138.0
84	76.5
85	15.0
86	11.0
87	7.0
88	6.5
89	5.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	762541.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.324462999317994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.91058549040241	15.1606735304251
2	13.094132526582397	5.8463895419571
3	5.68899326746809	3.810111591088816
4	3.0391893098058076	2.7139307717873016
5	1.766017980341958	1.9712701529137167
6	1.0981640660770111	1.4709553836190101
7	0.7494432850839584	1.171164322156091
8	0.5714653653716409	1.020612592370475
9	0.43734476423846413	0.8787138306468338
>10	3.727570596092504	17.996821295969482
>50	1.1107951014864734	17.945346506592774
>100	0.7933409340945274	24.65077341795329
>500	0.008834531560072688	1.4755147417406127
>1k	0.0029448438533575625	1.7345384488542295
>5k	0.001177937541343025	2.1531838719251715
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8469	1.1106288055330795	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	7907	1.0369278504368946	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3802	0.4985961410599561	No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	3010	0.39473287337992313	No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCTT	2791	0.36601310618052013	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	2328	0.3052950595443393	No Hit
TCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	1261	0.16536815725318377	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGGGAATATTCG	934	0.12248521718832169	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCG	924	0.12117381229337176	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	897	0.117633019077007	No Hit
CGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC	895	0.11737073809801703	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCT	871	0.11422336635013725	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	828	0.10858432530185262	No Hit
ACTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGCT	795	0.1042566891485179	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3754552214241595	0.0
2	0.0	0.0	0.0	1.3717295201176067	0.0
3	0.0	0.0	0.0	2.0834289566069235	0.0
4	0.0	0.0	0.0	2.942661443778105	0.0
5	0.0	0.0	0.0	5.242078786583279	0.0
6	0.0	0.0	0.0	7.012475394765659	0.0
7	0.0	0.0	0.0	8.364271560480027	0.0
8	0.0	0.0	0.0	11.09881304742958	0.0
9	0.0	0.0	0.0	12.376252555600288	0.0
10	0.0	0.0	0.0	13.937742364017147	0.0
11	0.0	0.0	0.0	16.38902039365752	0.0
12	0.0	0.0	0.0	17.73321041098118	0.0
13	0.0	0.0	0.0	18.299081623152066	0.0
14	0.0	0.0	0.0	18.532642834942646	0.0
15	0.0	0.0	0.0	18.917277890631453	0.0
16	0.0	0.0	0.0	19.71146469501312	0.0
17	0.0	0.0	0.0	20.6179077584025	0.0
18	0.0	0.0	0.0	21.4923525423551	0.0
19	0.0	0.0	0.0	22.02622547508921	0.0
20	0.0	0.0	0.0	22.64638884991102	0.0
21	1.3114048949499108E-4	0.0	0.0	23.284780752772637	0.0
22	2.6228097898998217E-4	0.0	0.0	23.94271258856901	0.0
23	2.6228097898998217E-4	0.0	0.0	24.580317648493654	0.0
24	2.6228097898998217E-4	0.0	0.0	25.049931741375218	0.0
25	2.6228097898998217E-4	0.0	0.0	25.527807685094967	0.0
26	2.6228097898998217E-4	0.0	0.0	25.9353923264454	0.0
27	2.6228097898998217E-4	0.0	0.0	26.42205468296131	0.0
28	2.6228097898998217E-4	0.0	0.0	26.843671356687707	0.0
29	3.934214684849733E-4	0.0	0.0	27.326006077050284	0.0
30	3.934214684849733E-4	0.0	0.0	28.278348311762908	0.0
31	3.934214684849733E-4	0.0	0.0	28.778255857717813	0.0
32	3.934214684849733E-4	0.0	0.0	29.135613691591665	0.0
33	3.934214684849733E-4	0.0	0.0	29.556574662870588	0.0
34	3.934214684849733E-4	0.0	0.0	30.035237449527305	0.0
35	3.934214684849733E-4	0.0	0.0	30.48636073339007	0.0
36	3.934214684849733E-4	0.0	0.0	30.874536582295246	0.0
37	3.934214684849733E-4	0.0	0.0	31.30834932154468	0.0
38	3.934214684849733E-4	0.0	0.0	31.77455376169937	0.0
39	3.934214684849733E-4	0.0	0.0	32.837840850524756	0.0
40	3.934214684849733E-4	0.0	0.0	33.23362284782064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGA	110	0.0	46.000004	4
TACGCGG	55	1.8189894E-12	46.000004	2
CTTGCGG	55	1.8189894E-12	46.000004	2
CGCTAGG	55	1.8189894E-12	46.000004	2
TCATCGA	115	0.0	46.000004	16
TCACGAG	45	3.092282E-10	46.0	1
TTCTACG	25	3.4160657E-5	46.0	1
CGGCGTT	20	6.3101115E-4	46.0	13
TCGTTGC	30	1.8605715E-6	46.0	42
CTCACGA	25	3.4160657E-5	46.0	27
CGAACGC	20	6.3101115E-4	46.0	21
ACACGAT	40	5.6079443E-9	46.0	23
GTCGATT	25	3.4160657E-5	46.0	14
GGTCGTA	20	6.3101115E-4	46.0	18
GTCGACC	25	3.4160657E-5	46.0	44
CAAGTCG	20	6.3101115E-4	46.0	1
CGCGGTA	20	6.3101115E-4	46.0	31
TTTACGT	50	1.6370905E-11	46.0	16
TTTACGC	20	6.3101115E-4	46.0	23
CACGGCA	40	5.6079443E-9	46.0	37
>>END_MODULE