FastQCFastQC Report
Sat 14 Jan 2017
SRR2933109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618936
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266521.6462664367214022No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC118220.7302326960423389No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG56500.3489946483369324No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCTT25960.16035223134206664Illumina Single End Adapter 2 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC25170.15547248316178033No Hit
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT20860.1288500595452816No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT20800.12847944575943707No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTT19870.12273493207884685No Hit
GGGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT18930.11692864943394922No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC18430.11384020121857812No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG17320.10698384618045433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG301.8618775E-646.01
ATCACGC206.3122227E-446.034
GTTACGC206.3122227E-446.022
TCGCTAG301.8618775E-646.01
ACTCGAT206.3122227E-446.029
ACGTCCG206.3122227E-446.023
ATACGCG206.3122227E-446.01
CGCAACG206.3122227E-446.023
AATCGCG301.8618775E-646.01
ACGCGTA206.3122227E-446.012
ATGCGTA351.01992555E-745.99999628
ACGTAAC351.01992555E-745.99999611
CGTTTTT77450.044.515171
CGGGATT8650.043.0751466
CATACGA4550.042.96703318
CACGGGA3550.042.7605634
ATACGAA4600.042.519
TTGCGAG1200.042.1666681
GCGGGAT11950.042.1506275
CGTTAGG1650.041.818182
Produced by FastQC (version 0.11.5)