FastQCFastQC Report
Sat 14 Jan 2017
SRR2933108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531457
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC172441.1259865605106771No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167861.0960803992537824No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG94910.6197366298890534No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT29720.19406356169321112Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT26490.17297253530461515Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT25490.16644280577254209Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA24010.15677880606507397No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT17480.11413967222063694No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCG206.312116E-446.039
CCTTACG206.312116E-446.01
ATCTCGA253.4176912E-546.010
TCGTTAC206.312116E-446.024
ACCGGTC301.861812E-646.021
TCGCACA206.312116E-446.033
CGAACGT253.4176912E-546.039
TCCGCAT206.312116E-446.011
ACGTTGC206.312116E-446.033
GCGAAGT206.312116E-446.016
GCGAACG253.4176912E-546.01
TTTCGCG301.861812E-646.01
TAGCGTA206.312116E-446.014
CGGCATA206.312116E-446.010
GCAAACG405.6134013E-946.01
CCTACGC206.312116E-446.032
TAACGTG206.312116E-446.034
AGGCGTA253.4176912E-546.012
CGTTCGA253.4176912E-546.026
TCGATAT253.4176912E-546.013
Produced by FastQC (version 0.11.5)