##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933108.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1531457
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.467820513406515	33.0	31.0	34.0	31.0	34.0
2	32.86214630903773	34.0	31.0	34.0	31.0	34.0
3	32.91379712260938	34.0	31.0	34.0	31.0	34.0
4	36.346146186278816	37.0	37.0	37.0	35.0	37.0
5	36.24475450502365	37.0	37.0	37.0	35.0	37.0
6	36.330154878654774	37.0	37.0	37.0	35.0	37.0
7	36.54166587765768	37.0	37.0	37.0	35.0	37.0
8	36.49494435690979	37.0	37.0	37.0	35.0	37.0
9	38.31706277094297	39.0	39.0	39.0	37.0	39.0
10	38.002334378307715	39.0	39.0	39.0	35.0	39.0
11	37.69246149255252	39.0	37.0	39.0	35.0	39.0
12	37.43737303757141	39.0	37.0	39.0	35.0	39.0
13	37.37076391958769	39.0	37.0	39.0	35.0	39.0
14	38.336597762784066	41.0	38.0	41.0	33.0	41.0
15	38.5835978417938	41.0	38.0	41.0	33.0	41.0
16	38.76430484172915	41.0	38.0	41.0	35.0	41.0
17	38.789474990156435	41.0	38.0	41.0	35.0	41.0
18	38.74243938941805	40.0	38.0	41.0	35.0	41.0
19	38.722930516495076	40.0	37.0	41.0	35.0	41.0
20	38.601904591509914	40.0	37.0	41.0	35.0	41.0
21	38.560299113850405	40.0	37.0	41.0	35.0	41.0
22	38.499146890836634	40.0	36.0	41.0	35.0	41.0
23	38.46993614577491	40.0	36.0	41.0	35.0	41.0
24	38.425553574145404	40.0	36.0	41.0	35.0	41.0
25	38.301731618974614	40.0	36.0	41.0	34.0	41.0
26	38.22945730764886	40.0	35.0	41.0	34.0	41.0
27	38.124986205946364	40.0	35.0	41.0	34.0	41.0
28	38.116244204048826	40.0	35.0	41.0	34.0	41.0
29	38.10220985636554	40.0	35.0	41.0	34.0	41.0
30	38.07553852311884	40.0	35.0	41.0	34.0	41.0
31	37.99254239590142	40.0	35.0	41.0	34.0	41.0
32	37.90354283535222	40.0	35.0	41.0	34.0	41.0
33	37.80046778982368	40.0	35.0	41.0	34.0	41.0
34	37.667421285742925	40.0	35.0	41.0	34.0	41.0
35	37.614538965181524	40.0	35.0	41.0	34.0	41.0
36	37.52975303909937	40.0	35.0	41.0	33.0	41.0
37	37.433416674447926	40.0	35.0	41.0	33.0	41.0
38	37.39369241186661	40.0	35.0	41.0	33.0	41.0
39	37.32021010057743	40.0	35.0	41.0	33.0	41.0
40	37.287238231305224	40.0	35.0	41.0	33.0	41.0
41	37.18726154243965	40.0	35.0	41.0	33.0	41.0
42	37.18202274043607	40.0	35.0	41.0	33.0	41.0
43	37.15120829380126	40.0	35.0	41.0	33.0	41.0
44	37.13956643901853	40.0	35.0	41.0	33.0	41.0
45	37.103980719014636	40.0	35.0	41.0	33.0	41.0
46	37.041558463606876	40.0	35.0	41.0	33.0	41.0
47	36.96274985193838	39.0	35.0	41.0	33.0	41.0
48	36.860637941515826	39.0	35.0	41.0	32.0	41.0
49	36.82305151238331	39.0	35.0	41.0	32.0	41.0
50	36.72803545904325	39.0	35.0	41.0	32.0	41.0
51	36.660424680549305	39.0	35.0	41.0	32.0	41.0
52	36.41775250627344	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	3.0
13	5.0
14	10.0
15	20.0
16	66.0
17	120.0
18	256.0
19	459.0
20	787.0
21	1365.0
22	2170.0
23	3278.0
24	5210.0
25	8002.0
26	10649.0
27	12131.0
28	13232.0
29	15007.0
30	17832.0
31	21791.0
32	27708.0
33	38610.0
34	94982.0
35	143325.0
36	91404.0
37	104773.0
38	176338.0
39	737806.0
40	4112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.43471543765185	23.86655322349893	27.199196582078372	12.49953475677084
2	35.42410919797291	23.98180294974002	26.799707729306142	13.794380122980925
3	31.617080988888357	24.509862176998766	29.778635639133196	14.094421194979684
4	28.094226609039623	28.3370019530421	29.095168849011106	14.473602588907164
5	25.292580856008364	32.86099446474828	26.826479620387644	15.019945058855717
6	26.71345000218746	39.74306820237199	23.54483344945369	9.99864834598686
7	89.31344464780925	1.3362438514434294	7.174997404432511	2.1753140963148168
8	87.1972246037597	1.6669746522429294	7.648206903621844	3.487593840375538
9	81.98219081567423	3.8099665873739843	10.00746348085516	4.200379116096633
10	42.72330205810545	31.9854230317926	14.524077398190089	10.76719751191186
11	26.52408784575734	23.56546739477504	33.914043946385696	15.996400813081921
12	27.783280888722306	19.951980369021136	33.159925482726585	19.104813259529976
13	27.805286077245395	22.45730699588692	34.44954706531101	15.287859861556674
14	17.827663460351808	25.56428290183792	36.646670458262946	19.96138317954732
15	15.046455760755933	30.286583299433158	37.71741550693229	16.949545432878622
16	25.831805920766953	27.77283332147099	31.413941103145564	14.981419654616488
17	23.771545658807266	26.33015487865477	28.594860972263668	21.303438490274296
18	20.050513987660118	26.42176698398976	34.8079639193265	18.719755109023627
19	20.60319029525478	28.611642377161097	29.662928831824857	21.12223849575927
20	18.363558363049044	35.84997815805471	27.46541365510099	18.32104982379525
21	22.306078459924112	29.030851013120184	32.83735684384217	15.825713683113532
22	19.698300376700097	24.853652436862415	34.21134253198098	21.23670465445651
23	19.040364829048418	25.836964407097295	34.68644565273461	20.436225111119672
24	19.415758979847293	28.212610605456113	32.12548573025556	20.246144684441024
25	21.195306169223166	27.953706829509418	31.040309979320348	19.810677021947072
26	18.965142344838934	30.877980903153013	26.987176264171964	23.169700487836092
27	20.779754181802033	27.922755911527386	31.302086836261157	19.99540307040942
28	16.874322948669143	30.418940917048275	28.950470042580367	23.756266091702216
29	19.650894540297244	27.957951153705263	28.855984856251272	23.53516944974622
30	23.017035411376224	25.89351186484505	28.44748497672478	22.641967747053947
31	21.729046261174815	26.43012503779081	29.439024406170073	22.401804294864302
32	23.11824621912336	25.80451165132289	26.60903962696961	24.46820250258414
33	22.762637148806657	26.508351197585046	29.272777492283492	21.4562341613248
34	18.985580398274323	26.06615791367306	31.505096127413307	23.44316556063931
35	20.25476392742336	28.888437612025676	27.914920236088903	22.941878224462066
36	23.056279085863984	27.248300148159565	27.426822953566436	22.26859781241001
37	20.635512456438544	28.80328993892744	28.728328643899243	21.83286896073478
38	22.30033229793589	27.275463823012984	29.135457280223996	21.288746598827128
39	20.54840586448069	29.22798354769347	27.957102288866093	22.26650829895975
40	24.710520765519373	26.520757683695983	29.265333600616927	19.503387950167717
41	20.438184029979293	26.279549474781206	27.986877855532345	25.295388639707156
42	19.313568712670353	27.344613658757638	31.436925751098464	21.904891877473542
43	19.022408072835216	27.386665116944194	31.965050275652533	21.62587653456806
44	21.50520713281535	27.146697556640508	27.89676758798974	23.451327722554403
45	19.480925680577386	26.069488075734416	27.922168235869506	26.5274180078187
46	21.905153066654826	26.001187104828933	28.870023774745224	23.223636053771017
47	18.777282026201192	26.15613758662502	30.866292687290596	24.200287699883184
48	19.0173148838002	29.123507875180305	29.564852294253118	22.29432494676638
49	22.92901465728388	25.44844549993895	29.661100507555876	21.961439335221293
50	20.323130195624167	26.29345779868452	29.45214916252954	23.931262843161775
51	19.982539503231237	26.59565368142886	27.952009099831077	25.469797715508825
52	19.636006756964118	26.998668588148412	30.260137894828258	23.105186760059212
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	880.0
1	2173.5
2	3467.0
3	11766.0
4	20065.0
5	11810.0
6	3555.0
7	4071.0
8	4587.0
9	6291.5
10	7996.0
11	10159.5
12	12323.0
13	12563.5
14	12025.0
15	11246.0
16	10421.5
17	9597.0
18	8471.5
19	7346.0
20	7561.5
21	7777.0
22	8100.0
23	8423.0
24	9565.5
25	10708.0
26	13017.5
27	15327.0
28	17486.5
29	19646.0
30	23157.0
31	26668.0
32	31197.0
33	35726.0
34	39814.0
35	43902.0
36	46961.0
37	50020.0
38	52703.0
39	58595.0
40	61804.0
41	67099.5
42	72395.0
43	81553.0
44	90711.0
45	92741.0
46	94771.0
47	108983.0
48	123195.0
49	134875.5
50	146556.0
51	143924.5
52	141293.0
53	132032.5
54	122772.0
55	104548.0
56	86324.0
57	77214.0
58	68104.0
59	67083.0
60	66062.0
61	50091.5
62	34121.0
63	26850.0
64	17152.5
65	14726.0
66	11609.0
67	8492.0
68	7066.0
69	5640.0
70	4228.5
71	2817.0
72	2202.5
73	1588.0
74	1503.0
75	1418.0
76	1149.0
77	880.0
78	648.5
79	417.0
80	290.0
81	163.0
82	118.0
83	73.0
84	73.0
85	73.0
86	44.5
87	16.0
88	11.0
89	6.5
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1531457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.14958569769319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.89932894787391	13.385431898985578
2	11.994397398868585	4.593754817636445
3	4.989462880540722	2.86638441049121
4	2.6076902207000137	1.9974474941732552
5	1.5633538897527837	1.4968789643821458
6	1.0241197154560235	1.176688095349337
7	0.7075096445594485	0.9483961599304926
8	0.5356952671997752	0.8206673941672599
9	0.41278478875874364	0.7114191918335766
>10	3.669133338529318	15.61425506482503
>50	1.371367474749786	19.32018258240271
>100	1.2118022963739086	31.806632622376874
>500	0.008902757758038863	1.162636145543173
>1k	0.0034241375992457167	1.245517657469549
>5k	3.424137599245717E-4	0.622332618428191
>10k+	6.848275198491434E-4	2.231374882005199
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	17244	1.1259865605106771	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16786	1.0960803992537824	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	9491	0.6197366298890534	No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCTT	2972	0.19406356169321112	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	2649	0.17297253530461515	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT	2549	0.16644280577254209	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2401	0.15677880606507397	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT	1748	0.11413967222063694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20059329122528416	0.0
2	0.0	0.0	0.0	0.6599597638066234	0.0
3	0.0	0.0	0.0	1.0254940230120728	0.0
4	0.0	0.0	0.0	1.4822486037805829	0.0
5	0.0	0.0	0.0	2.7670381865112765	0.0
6	0.0	0.0	0.0	4.013432959593381	0.0
7	0.0	0.0	0.0	5.04304071221066	0.0
8	0.0	0.0	0.0	6.918836114889285	0.0
9	0.0	0.0	0.0	7.881514139802815	0.0
10	0.0	0.0	0.0	8.86972340718675	0.0
11	0.0	0.0	0.0	10.35699990270703	0.0
12	0.0	0.0	0.0	11.245696092022172	0.0
13	0.0	0.0	0.0	11.621547323888297	0.0
14	0.0	0.0	0.0	11.836571317379462	0.0
15	0.0	0.0	0.0	12.087182336820426	0.0
16	0.0	0.0	0.0	12.52728610728215	0.0
17	0.0	0.0	0.0	13.076697550110778	0.0
18	0.0	0.0	0.0	13.642302722178945	0.0
19	0.0	0.0	0.0	14.003135576121302	0.0
20	0.0	0.0	0.0	14.447548968074193	0.0
21	0.0	0.0	0.0	14.875768630787544	0.0
22	0.0	0.0	0.0	15.354201913602537	0.0
23	0.0	0.0	0.0	15.802924927046597	0.0
24	0.0	0.0	0.0	16.1286278361064	0.0
25	6.529729532073053E-5	0.0	0.0	16.46719431234439	0.0
26	6.529729532073053E-5	0.0	0.0	16.755481871185413	0.0
27	6.529729532073053E-5	0.0	0.0	17.12349742761305	0.0
28	6.529729532073053E-5	0.0	0.0	17.42837049946554	0.0
29	6.529729532073053E-5	0.0	0.0	17.822831460498076	0.0
30	6.529729532073053E-5	0.0	0.0	18.783093485484738	0.0
31	6.529729532073053E-5	0.0	0.0	19.20256331062511	0.0
32	6.529729532073053E-5	0.0	0.0	19.524152490079707	0.0
33	6.529729532073053E-5	0.0	0.0	19.857495182692038	0.0
34	6.529729532073053E-5	0.0	0.0	20.223421225669412	0.0
35	6.529729532073053E-5	0.0	0.0	20.60965472749153	0.0
36	6.529729532073053E-5	0.0	0.0	20.919947474855643	0.0
37	6.529729532073053E-5	0.0	0.0	21.26171351856435	0.0
38	6.529729532073053E-5	0.0	0.0	21.679159127549777	0.0
39	6.529729532073053E-5	0.0	0.0	22.90831541466721	0.0
40	1.3059459064146105E-4	0.0	0.0	23.26333680932602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACTCG	20	6.312116E-4	46.0	39
CCTTACG	20	6.312116E-4	46.0	1
ATCTCGA	25	3.4176912E-5	46.0	10
TCGTTAC	20	6.312116E-4	46.0	24
ACCGGTC	30	1.861812E-6	46.0	21
TCGCACA	20	6.312116E-4	46.0	33
CGAACGT	25	3.4176912E-5	46.0	39
TCCGCAT	20	6.312116E-4	46.0	11
ACGTTGC	20	6.312116E-4	46.0	33
GCGAAGT	20	6.312116E-4	46.0	16
GCGAACG	25	3.4176912E-5	46.0	1
TTTCGCG	30	1.861812E-6	46.0	1
TAGCGTA	20	6.312116E-4	46.0	14
CGGCATA	20	6.312116E-4	46.0	10
GCAAACG	40	5.6134013E-9	46.0	1
CCTACGC	20	6.312116E-4	46.0	32
TAACGTG	20	6.312116E-4	46.0	34
AGGCGTA	25	3.4176912E-5	46.0	12
CGTTCGA	25	3.4176912E-5	46.0	26
TCGATAT	25	3.4176912E-5	46.0	13
>>END_MODULE