FastQCFastQC Report
Sat 14 Jan 2017
SRR2933107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027686
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC153281.4915061604420026No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134241.3062355622242592No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG81910.7970333350848411No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT25040.2436541900930829Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT19770.19237393522924318Illumina Single End Adapter 2 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA18860.18351909046148338No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT18000.17515077562601805Illumina Single End Adapter 2 (95% over 22bp)
GGGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT14660.14265057614874582No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG13740.13369842539452714No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCACCATAGATCG13470.13107116376013686No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA12350.12017289327674018No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11580.11268033231940494No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCACCATAGAT10900.10606352524019982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC253.416901E-546.034
TCGTTAG301.86121E-646.01
GCGAATA253.416901E-546.013
ACGTTAG253.416901E-546.01
CGTGACA253.416901E-546.012
ATTACGT206.311142E-446.012
ATTACCG253.416901E-546.01
ATTCGTG253.416901E-546.015
CGACATA206.311142E-446.014
ATCGTTC206.311142E-446.026
CCCGTAG206.311142E-446.020
TACGCAA253.416901E-546.015
CTACGTA253.416901E-546.030
CGGATAA206.311142E-446.023
CTACGCG253.416901E-546.01
GCGATGT405.6097633E-946.09
CGTACGT206.311142E-446.023
GTATCGC206.311142E-446.09
TTAACGC253.416901E-546.028
ACTCGCG206.311142E-446.037
Produced by FastQC (version 0.11.5)