##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933107.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1027686
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46018628258048	33.0	31.0	34.0	31.0	34.0
2	32.83115465229652	34.0	31.0	34.0	31.0	34.0
3	32.87601076593434	34.0	31.0	34.0	31.0	34.0
4	36.329910108729706	37.0	37.0	37.0	35.0	37.0
5	36.20473957998844	37.0	37.0	37.0	35.0	37.0
6	36.292957187312076	37.0	37.0	37.0	35.0	37.0
7	36.50718118180067	37.0	37.0	37.0	35.0	37.0
8	36.45267815266531	37.0	37.0	37.0	35.0	37.0
9	38.28064214166584	39.0	39.0	39.0	37.0	39.0
10	37.96496497957548	39.0	38.0	39.0	35.0	39.0
11	37.641899373933285	39.0	37.0	39.0	35.0	39.0
12	37.289973785767245	39.0	35.0	39.0	35.0	39.0
13	37.164546369221725	39.0	37.0	39.0	34.0	39.0
14	38.0218072446253	40.0	37.0	41.0	33.0	41.0
15	38.29108891237207	40.0	37.0	41.0	33.0	41.0
16	38.509199307959825	40.0	37.0	41.0	34.0	41.0
17	38.55488057636282	40.0	37.0	41.0	35.0	41.0
18	38.48169869006681	40.0	37.0	41.0	35.0	41.0
19	38.43169217056572	40.0	36.0	41.0	35.0	41.0
20	38.262654157009045	40.0	35.0	41.0	34.0	41.0
21	38.224408039031374	40.0	35.0	41.0	34.0	41.0
22	38.15838884639861	40.0	35.0	41.0	34.0	41.0
23	38.135797315522446	40.0	35.0	41.0	34.0	41.0
24	38.08156966232877	40.0	35.0	41.0	34.0	41.0
25	37.93958271300767	40.0	35.0	41.0	34.0	41.0
26	37.836641736872934	40.0	35.0	41.0	34.0	41.0
27	37.74398503044704	40.0	35.0	41.0	34.0	41.0
28	37.70804895658791	40.0	35.0	41.0	34.0	41.0
29	37.65883158863699	40.0	35.0	41.0	34.0	41.0
30	37.61162164318673	40.0	35.0	41.0	34.0	41.0
31	37.479647479872256	40.0	35.0	41.0	33.0	41.0
32	37.34602300702744	40.0	35.0	41.0	33.0	41.0
33	37.22746539312592	40.0	35.0	41.0	33.0	41.0
34	37.04964746041106	40.0	35.0	41.0	33.0	41.0
35	36.98214337842493	40.0	35.0	41.0	33.0	41.0
36	36.899576329734955	40.0	35.0	41.0	32.0	41.0
37	36.773067843679875	40.0	35.0	41.0	32.0	41.0
38	36.775430433031104	39.0	35.0	41.0	32.0	41.0
39	36.719146704343544	39.0	35.0	41.0	32.0	41.0
40	36.640790085687655	39.0	35.0	41.0	32.0	41.0
41	36.47434041137079	39.0	35.0	41.0	31.0	41.0
42	36.49425018926014	39.0	35.0	41.0	31.0	41.0
43	36.443112974196396	39.0	35.0	41.0	31.0	41.0
44	36.411613080259926	39.0	35.0	41.0	31.0	41.0
45	36.341055536418715	39.0	35.0	41.0	31.0	41.0
46	36.27355437361217	39.0	35.0	41.0	31.0	41.0
47	36.18073030088957	38.0	35.0	41.0	31.0	41.0
48	36.07266713762764	38.0	35.0	41.0	31.0	41.0
49	36.03190079460069	38.0	35.0	41.0	31.0	41.0
50	35.91474438690417	38.0	35.0	41.0	31.0	41.0
51	35.81690321751975	38.0	35.0	41.0	30.0	41.0
52	35.558035236443814	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	4.0
14	13.0
15	35.0
16	46.0
17	122.0
18	257.0
19	402.0
20	760.0
21	1153.0
22	1864.0
23	2895.0
24	4714.0
25	7283.0
26	9844.0
27	10883.0
28	11383.0
29	12222.0
30	14091.0
31	16855.0
32	21604.0
33	29601.0
34	76641.0
35	108984.0
36	67875.0
37	76615.0
38	117902.0
39	430264.0
40	3370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.46787832081005	22.13059241830676	26.263177663216197	13.138351597666992
2	37.70743203663376	23.43945524216541	25.759911101250772	13.093201619950062
3	32.80739447652298	23.67950911075951	29.720264750127956	13.792831662589547
4	29.26574848737844	26.88661711845836	29.277717123712886	14.569917270450313
5	26.417699569712926	31.213717030299136	27.273797638578323	15.094785761409613
6	27.804796406684535	38.074762135516096	25.09025130244063	9.030190155358737
7	86.38971436800735	1.7766127007665766	9.373096451639897	2.460576479586177
8	83.78123278900364	2.462522599315355	9.854955696584366	3.9012889150966346
9	78.536440118869	4.323986120274091	12.38277061281364	4.756803148043274
10	47.58710345377868	22.407622561755243	17.611994325114868	12.393279659351203
11	31.242616908277434	25.223463197902863	28.371603777807618	15.16231611601209
12	30.697119548188844	20.308537821863876	30.90253248560358	18.0918101443437
13	28.20511323497644	22.591238958203185	34.20480574805923	14.998842058761138
14	19.567941958925196	23.990304431509234	37.231313844890366	19.2104397646752
15	15.960127898988603	29.49908824290688	37.25106696014152	17.289716897962997
16	27.097673803087712	27.04152824890093	30.268389371850933	15.592408576160421
17	25.229982698995606	25.757089227643466	27.166177217554775	21.846750855806153
18	22.413947450875074	25.30763287618981	33.957940460413006	18.320479212522113
19	22.232179868169847	27.536913025963184	28.346985363233518	21.88392174263345
20	18.34266497743474	36.29523025515576	27.230691086577032	18.131413680832473
21	23.377665940764008	28.46842323433422	32.34314761512758	15.810763209774192
22	20.493711114095163	24.658407334536037	34.213174062894694	20.634707488474106
23	20.315446546902457	25.76993361785604	33.33917169252087	20.57544814272064
24	21.778928583244298	29.144213310291278	29.75169458375418	19.325163522710245
25	22.14392333845163	27.250541507814646	30.60214890540496	20.00338624832877
26	18.566274134317293	30.978333849055062	27.229328802766606	23.226063213861043
27	22.08096636521272	27.273992250551238	30.372896001307794	20.272145382928247
28	17.618027296275322	30.465628606403122	27.417615886564572	24.49872821075698
29	20.564063342304944	29.614590448833596	26.862485233816557	22.958860975044907
30	23.851254176859467	27.24441123066773	26.38490745227628	22.51942714019652
31	21.392039981083716	28.088443357212224	28.67811763515315	21.84139902655091
32	23.54289150577122	29.116286492177572	23.884435518241954	23.456386483809258
33	22.97180267124394	27.744369389093553	27.35787001087881	21.925957928783696
34	17.724577351447817	28.295997026329054	31.076612895378545	22.902812726844584
35	20.284795161167906	32.665035818333614	25.948490103008115	21.101678917490364
36	23.21847334691725	28.960499607856878	25.71330153373696	22.10772551148892
37	19.150110053070684	32.759422625198745	26.717888537938634	21.372578783791937
38	22.096924546991982	29.04972919743968	28.348834176976233	20.5045120785921
39	19.976043266133818	31.835210365812127	26.24624642157235	21.942499946481707
40	25.028656613012146	28.491290141152064	27.67051414537125	18.80953910046454
41	20.513366923359857	28.217471095256723	25.91637912747668	25.352782853906735
42	19.637223821283932	29.61303355305025	28.746718355606678	22.003024270059143
43	19.29830707044759	28.821935883139403	29.843064905039085	22.03669214137392
44	21.977043571674617	27.80859134015643	27.19517440151953	23.019190686649424
45	19.691909785673833	27.740185231675824	25.757770369548677	26.810134613101667
46	22.513394169036065	26.59226650942019	27.659810486860774	23.23452883468297
47	18.194857184003673	27.091738137913723	30.60292735329663	24.110477324785975
48	18.276107682696853	29.99836525942749	28.676755351342724	23.048771706532932
49	23.022888314134864	27.1500244238026	28.34863956500332	21.47844769705922
50	21.62576896055799	26.420618749306694	28.343385041734535	23.610227248400776
51	19.8839918029437	27.562796418361252	26.26376149913495	26.2894502795601
52	20.253851857473975	28.712369342386683	28.246273667248555	22.787505132890786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	738.0
1	2371.5
2	4005.0
3	10445.0
4	16885.0
5	10329.0
6	3773.0
7	4232.5
8	4692.0
9	6023.0
10	7354.0
11	8815.0
12	10276.0
13	10751.0
14	10224.0
15	9222.0
16	8540.0
17	7858.0
18	7117.5
19	6377.0
20	5915.5
21	5454.0
22	5260.0
23	5066.0
24	5651.0
25	6236.0
26	7104.0
27	7972.0
28	8498.5
29	9025.0
30	10219.0
31	11413.0
32	13677.0
33	15941.0
34	17655.5
35	19370.0
36	22413.5
37	25457.0
38	26526.0
39	31247.0
40	34899.0
41	41410.5
42	47922.0
43	56541.5
44	65161.0
45	77663.0
46	90165.0
47	92432.0
48	94699.0
49	95863.5
50	97028.0
51	85188.0
52	73348.0
53	65805.5
54	58263.0
55	56701.5
56	55140.0
57	51386.5
58	47633.0
59	49333.5
60	51034.0
61	39480.5
62	27927.0
63	24019.5
64	17932.5
65	15753.0
66	13814.0
67	11875.0
68	9399.0
69	6923.0
70	6192.0
71	5461.0
72	4259.5
73	3058.0
74	2500.5
75	1943.0
76	1597.0
77	1251.0
78	1080.5
79	910.0
80	808.0
81	706.0
82	553.0
83	400.0
84	220.5
85	41.0
86	58.0
87	75.0
88	43.0
89	10.0
90	9.0
91	6.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1027686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.438855199784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33629703077253	16.720411365930588
2	11.485597578431891	5.384185170477098
3	4.700759639737097	3.3054127357435985
4	2.4382943317895838	2.2860331110908056
5	1.498091019642821	1.7556769242752421
6	0.9546785046767519	1.342594274007882
7	0.7006110370447097	1.1495064454063018
8	0.5569496588877643	1.044340992659152
9	0.4282700137514272	0.9034342954856285
>10	4.040920274551182	21.143302908886756
>50	1.3207960988155072	22.694422409581602
>100	0.5258185093706361	15.91795208548921
>500	0.007083133644453893	1.0989583411639012
>1k	0.004583204122881932	1.6459472035880558
>5k	4.1665492026199375E-4	0.7999260439414836
>10k+	8.333098405239875E-4	2.8078956922726817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	15328	1.4915061604420026	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13424	1.3062355622242592	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	8191	0.7970333350848411	No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	2504	0.2436541900930829	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	1977	0.19237393522924318	Illumina Single End Adapter 2 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1886	0.18351909046148338	No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	1800	0.17515077562601805	Illumina Single End Adapter 2 (95% over 22bp)
GGGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	1466	0.14265057614874582	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCG	1374	0.13369842539452714	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCACCATAGATCG	1347	0.13107116376013686	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1235	0.12017289327674018	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1158	0.11268033231940494	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCACCATAGAT	1090	0.10606352524019982	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2528982588066783	0.0
2	0.0	0.0	0.0	0.7662846433638291	0.0
3	0.0	0.0	0.0	1.1651418818588557	0.0
4	0.0	0.0	0.0	1.6359082443470088	0.0
5	0.0	0.0	0.0	3.0267027088040512	0.0
6	0.0	0.0	0.0	4.287107151406169	0.0
7	0.0	0.0	0.0	5.34044445482375	0.0
8	0.0	0.0	0.0	7.294056744959064	0.0
9	0.0	0.0	0.0	8.290956576230483	0.0
10	0.0	0.0	0.0	9.389833081310828	0.0
11	0.0	0.0	0.0	11.079259618210232	0.0
12	0.0	0.0	0.0	12.035777465101209	0.0
13	9.730598645889892E-5	0.0	0.0	12.471902896409993	0.0
14	9.730598645889892E-5	0.0	0.0	12.69667972513005	0.0
15	9.730598645889892E-5	0.0	0.0	13.001052850773485	0.0
16	9.730598645889892E-5	0.0	0.0	13.532538148811991	0.0
17	9.730598645889892E-5	0.0	0.0	14.137392160640507	0.0
18	9.730598645889892E-5	0.0	0.0	14.821550551433026	0.0
19	9.730598645889892E-5	0.0	0.0	15.253978355256372	0.0
20	9.730598645889892E-5	0.0	0.0	15.771840815190632	0.0
21	9.730598645889892E-5	0.0	0.0	16.30498031499894	0.0
22	9.730598645889892E-5	0.0	0.0	16.835395247186398	0.0
23	9.730598645889892E-5	0.0	0.0	17.34965738562168	0.0
24	9.730598645889892E-5	0.0	0.0	17.719322828179035	0.0
25	1.9461197291779784E-4	0.0	0.0	18.14941528832737	0.0
26	1.9461197291779784E-4	0.0	0.0	18.507404012509657	0.0
27	1.9461197291779784E-4	0.0	0.0	18.965034066825858	0.0
28	1.9461197291779784E-4	0.0	0.0	19.34141362244888	0.0
29	2.919179593766968E-4	0.0	0.0	19.812082678950574	0.0
30	2.919179593766968E-4	0.0	0.0	21.028894039619104	0.0
31	2.919179593766968E-4	0.0	0.0	21.51532666592714	0.0
32	2.919179593766968E-4	0.0	0.0	21.851421543156178	0.0
33	2.919179593766968E-4	0.0	0.0	22.261858194039814	0.0
34	2.919179593766968E-4	0.0	0.0	22.715887926857036	0.0
35	2.919179593766968E-4	0.0	0.0	23.161549344838793	0.0
36	2.919179593766968E-4	0.0	0.0	23.510196694321028	0.0
37	2.919179593766968E-4	0.0	0.0	23.90934585077543	0.0
38	2.919179593766968E-4	0.0	0.0	24.386145184424038	0.0
39	2.919179593766968E-4	0.0	0.0	25.783264538000907	0.0
40	2.919179593766968E-4	0.0	0.0	26.190684703304317	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	25	3.416901E-5	46.0	34
TCGTTAG	30	1.86121E-6	46.0	1
GCGAATA	25	3.416901E-5	46.0	13
ACGTTAG	25	3.416901E-5	46.0	1
CGTGACA	25	3.416901E-5	46.0	12
ATTACGT	20	6.311142E-4	46.0	12
ATTACCG	25	3.416901E-5	46.0	1
ATTCGTG	25	3.416901E-5	46.0	15
CGACATA	20	6.311142E-4	46.0	14
ATCGTTC	20	6.311142E-4	46.0	26
CCCGTAG	20	6.311142E-4	46.0	20
TACGCAA	25	3.416901E-5	46.0	15
CTACGTA	25	3.416901E-5	46.0	30
CGGATAA	20	6.311142E-4	46.0	23
CTACGCG	25	3.416901E-5	46.0	1
GCGATGT	40	5.6097633E-9	46.0	9
CGTACGT	20	6.311142E-4	46.0	23
GTATCGC	20	6.311142E-4	46.0	9
TTAACGC	25	3.416901E-5	46.0	28
ACTCGCG	20	6.311142E-4	46.0	37
>>END_MODULE