FastQCFastQC Report
Sat 14 Jan 2017
SRR2933106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412416
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177671.2579155149757577No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC134410.9516318138565408No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG69980.4954630930264171No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT29370.20794156962254748Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT23530.16659397797815942Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT22190.15710668811454984Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA22120.15661108342018218No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA16710.11830792061262405No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG15010.10627180660655218No Hit
GGGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT14910.10556379990031267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG700.046.0000041
TCCCGAA351.0197982E-746.00000429
ACGTCCC351.0197982E-746.00000443
CTCGACC351.0197982E-746.00000433
TGTACGC351.0197982E-746.00000416
CGTCCCA351.0197982E-746.00000444
TCGCCTG206.3119474E-446.042
CTGTCGA253.417556E-546.042
TCGTTAG501.6370905E-1146.01
TCGTTAC253.417556E-546.021
AGATCCG301.8617084E-646.019
GTCGACA206.3119474E-446.010
GTCGAAA253.417556E-546.044
GGTCGAC206.3119474E-446.09
CGACGAT253.417556E-546.020
ACGTTCC253.417556E-546.014
GACGTAC206.3119474E-446.013
CCGGATA206.3119474E-446.034
TCGGGTA206.3119474E-446.026
ACATTCG206.3119474E-446.017
Produced by FastQC (version 0.11.5)