##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412416
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.508273058362406	33.0	31.0	34.0	31.0	34.0
2	32.88329288255019	34.0	31.0	34.0	31.0	34.0
3	32.93012681816122	34.0	31.0	34.0	31.0	34.0
4	36.367358483619554	37.0	37.0	37.0	35.0	37.0
5	36.2849266788255	37.0	37.0	37.0	35.0	37.0
6	36.36923257737097	37.0	37.0	37.0	35.0	37.0
7	36.56948165413023	37.0	37.0	37.0	35.0	37.0
8	36.51809240337124	37.0	37.0	37.0	35.0	37.0
9	38.331771234537136	39.0	39.0	39.0	37.0	39.0
10	38.033870332819795	39.0	39.0	39.0	35.0	39.0
11	37.718046241334	39.0	37.0	39.0	35.0	39.0
12	37.454587033848384	39.0	37.0	39.0	35.0	39.0
13	37.393417378449406	39.0	37.0	39.0	35.0	39.0
14	38.37368593955322	41.0	38.0	41.0	33.0	41.0
15	38.60592700734061	41.0	38.0	41.0	33.0	41.0
16	38.778220439304	41.0	38.0	41.0	35.0	41.0
17	38.76642292355793	41.0	38.0	41.0	35.0	41.0
18	38.739478312338576	40.0	38.0	41.0	35.0	41.0
19	38.71749541211654	41.0	37.0	41.0	35.0	41.0
20	38.61852457066473	41.0	37.0	41.0	35.0	41.0
21	38.56544459989125	40.0	36.0	41.0	35.0	41.0
22	38.51745661334904	40.0	36.0	41.0	35.0	41.0
23	38.48551205877022	40.0	36.0	41.0	35.0	41.0
24	38.464921807739366	40.0	36.0	41.0	35.0	41.0
25	38.34755695205945	40.0	35.0	41.0	35.0	41.0
26	38.2647739759391	40.0	35.0	41.0	35.0	41.0
27	38.17311259572251	40.0	35.0	41.0	34.0	41.0
28	38.16084213149667	40.0	35.0	41.0	34.0	41.0
29	38.121851494177356	40.0	35.0	41.0	34.0	41.0
30	38.08164662535683	40.0	35.0	41.0	34.0	41.0
31	37.98013828786986	40.0	35.0	41.0	34.0	41.0
32	37.91554896007975	40.0	35.0	41.0	34.0	41.0
33	37.816170306765144	40.0	35.0	41.0	34.0	41.0
34	37.68062100684217	40.0	35.0	41.0	34.0	41.0
35	37.61055170714577	40.0	35.0	41.0	34.0	41.0
36	37.53670377565816	40.0	35.0	41.0	33.0	41.0
37	37.447058090534235	40.0	35.0	41.0	33.0	41.0
38	37.398337317051066	40.0	35.0	41.0	33.0	41.0
39	37.371226324255744	40.0	35.0	41.0	33.0	41.0
40	37.32538218202003	40.0	35.0	41.0	33.0	41.0
41	37.23075991775794	40.0	35.0	41.0	33.0	41.0
42	37.24665820834655	40.0	35.0	41.0	33.0	41.0
43	37.2092414699352	40.0	35.0	41.0	33.0	41.0
44	37.17541432552449	40.0	35.0	41.0	33.0	41.0
45	37.121672368480674	40.0	35.0	41.0	33.0	41.0
46	37.045683424713395	40.0	35.0	41.0	33.0	41.0
47	36.94372267094114	39.0	35.0	41.0	33.0	41.0
48	36.896635976935976	39.0	35.0	41.0	33.0	41.0
49	36.86228632357606	39.0	35.0	41.0	33.0	41.0
50	36.75422963206307	39.0	35.0	41.0	32.0	41.0
51	36.67248388576736	39.0	35.0	41.0	32.0	41.0
52	36.45415090171734	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	10.0
14	5.0
15	27.0
16	55.0
17	117.0
18	208.0
19	396.0
20	670.0
21	1055.0
22	1733.0
23	2781.0
24	4538.0
25	7177.0
26	9485.0
27	10953.0
28	12135.0
29	13294.0
30	16042.0
31	19830.0
32	25206.0
33	35256.0
34	86399.0
35	139265.0
36	87341.0
37	95249.0
38	155881.0
39	681430.0
40	5874.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.26927194254384	23.82322205355929	26.93696474693008	12.970541256966786
2	33.900210702795775	24.05339503375776	28.4998187502832	13.546575513163262
3	30.703135620100593	25.003681634872443	30.031874461914903	14.261308283112056
4	26.542534210884046	28.317719425438398	30.506309755766008	14.633436607911552
5	24.99178712220762	32.70233415650913	26.733625220898094	15.572253500385155
6	26.628769427704018	39.644977117223256	24.191456341474467	9.53479711359826
7	89.80293341338529	1.1769903484525805	6.974361661153654	2.0457145770084737
8	87.82745310163578	1.5301440935248538	7.257422742308216	3.3849800625311524
9	82.65086206896551	3.800792401105623	9.537204336399475	4.011141193529385
10	43.51140173999728	32.93151592731887	13.698513752322262	9.858568580361593
11	26.36524933164167	24.289232067606147	33.52121471294576	15.824303887806426
12	28.777215777787845	19.73150969685985	31.933722076215503	19.557552449136796
13	27.669326883864244	24.11839004939055	32.99835176038787	15.213931306357335
14	17.735780393311888	24.726001404685306	36.84530619874031	20.692912003262496
15	15.282183152838824	30.6555575694413	37.68776337849472	16.37449589922516
16	26.190300874529882	28.849857265848023	30.412711269201143	14.547130590420954
17	23.24039093298292	27.363467986768768	28.968377588472517	20.427763491775792
18	20.61920850514296	26.608732837917444	32.96833227604331	19.80372638089628
19	19.87396064615524	30.353167905206398	29.122015043726496	20.65085640491187
20	18.743840341655716	36.92141691966106	26.960328968235984	17.374413770447234
21	22.40919105985772	30.344601024060903	31.137497734378538	16.10871018170284
22	19.619857039285876	25.226491345326025	32.668420635280256	22.485230980107843
23	17.98202512574199	28.3209762562871	33.72427103629526	19.972727581675652
24	18.915956771942543	30.423331369794738	30.936707032489014	19.72400482577371
25	22.087402011871855	28.393405342335402	30.03506049209298	19.48413215369976
26	18.623974806289365	32.50012744120712	26.52327642847433	22.352621324029183
27	20.096416353255698	28.563680955186005	30.459014907789207	20.880887783769086
28	16.475670057546786	31.528034233540257	27.975327382300964	24.02096832661199
29	19.10683537994472	29.412651796637817	27.994939168063798	23.485573655353665
30	24.805510557796005	27.501600095156103	26.83656939598532	20.856319951062577
31	21.104193098917033	28.34356167021614	29.464407086863925	21.087838144002898
32	23.23401887262676	27.016049096017035	26.122757034754635	23.627174996601568
33	23.436862793964384	27.218680615342787	26.939655172413797	22.404801418279032
34	18.644719402782183	28.6281095654538	30.278048393674382	22.449122638089626
35	19.32808747564457	30.59983744166025	27.54443450088359	22.527640581811593
36	22.83165866147084	30.08504576555349	24.393521455435227	22.68977411754044
37	19.248365920521998	32.74311534278853	26.086577892065794	21.92194084462368
38	21.886894512664824	28.464418414971227	27.77637749784766	21.87230957451629
39	19.766202025465585	30.548436154787257	25.848121233404324	23.837240586342833
40	23.811681544247588	29.598007952331322	27.648086682677057	18.94222382074403
41	19.711827110426388	28.774171348951015	26.601935973537543	24.91206556708505
42	18.92466525442929	30.290934115727946	28.93488887126739	21.849511758575378
43	19.130057999909376	28.507394422039965	29.844677488785177	22.517870089265486
44	21.103131088857673	28.067580656123976	27.568860732248858	23.260427522769493
45	19.19462821151842	26.466423489963297	26.751325388554083	27.587622909964203
46	22.48678859486157	27.220096628755268	27.22767230051203	23.06544247587113
47	18.15322114731071	27.667202863745526	31.25608885767366	22.923487131270107
48	19.549481172685667	29.565439643844304	28.055969346141644	22.829109837328378
49	22.07437468847705	27.719170555983506	28.441762200371564	21.764692555167883
50	20.604765168335675	26.619140536499163	28.59185962209434	24.184234673070822
51	19.373116702161404	27.379681340341655	27.04174973945353	26.20545221804341
52	19.77384849789297	27.68143379854094	29.23678293080792	23.30793477275817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	703.0
1	1839.0
2	2975.0
3	11967.5
4	20960.0
5	12165.5
6	3371.0
7	4031.5
8	4692.0
9	6412.5
10	8133.0
11	10317.5
12	12502.0
13	13217.0
14	12884.5
15	11837.0
16	10349.5
17	8862.0
18	8277.5
19	7693.0
20	8001.0
21	8309.0
22	8156.5
23	8004.0
24	8711.5
25	9419.0
26	11919.5
27	14420.0
28	18121.5
29	21823.0
30	23180.0
31	24537.0
32	26672.5
33	28808.0
34	34059.5
35	39311.0
36	41855.5
37	44400.0
38	47468.0
39	54657.0
40	58778.0
41	63018.0
42	67258.0
43	70841.0
44	74424.0
45	78885.5
46	83347.0
47	93185.5
48	103024.0
49	121739.0
50	140454.0
51	139675.0
52	138896.0
53	127877.0
54	116858.0
55	101009.0
56	85160.0
57	75099.0
58	65038.0
59	61030.5
60	57023.0
61	43048.5
62	29074.0
63	23903.5
64	14685.5
65	10638.0
66	8642.5
67	6647.0
68	4930.0
69	3213.0
70	2647.5
71	2082.0
72	1752.0
73	1422.0
74	1756.5
75	2091.0
76	1964.5
77	1838.0
78	1334.0
79	830.0
80	536.0
81	242.0
82	166.5
83	91.0
84	51.0
85	11.0
86	9.0
87	7.0
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1412416.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.3256892447781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0590270765604	14.036723239048833
2	12.370339805173725	5.028713654645399
3	5.073457720112887	3.0936457504660475
4	2.771116852757791	2.2529944004048943
5	1.6272023233031712	1.6537004380920601
6	1.0650817032188014	1.2989111831954598
7	0.7228326085348088	1.0284449683949604
8	0.551389994383996	0.8965905342823235
9	0.42523340957202843	0.7778845925512649
>10	4.051021622009126	18.71694282276194
>50	1.331719311116274	19.58862632090137
>100	0.9375889282991817	26.327927475363662
>500	0.009092619222591835	1.217140726214775
>1k	0.003846877363404238	1.3659868793826626
>5k	3.4971612394583984E-4	0.4974333237196166
>10k+	6.994322478916797E-4	2.2183336905747066
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17767	1.2579155149757577	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	13441	0.9516318138565408	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6998	0.4954630930264171	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	2937	0.20794156962254748	Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	2353	0.16659397797815942	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	2219	0.15710668811454984	Illumina Single End Adapter 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2212	0.15661108342018218	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1671	0.11830792061262405	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	1501	0.10627180660655218	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	1491	0.10556379990031267	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21325161991934388	0.0
2	0.0	0.0	0.0	0.6575966287552676	0.0
3	0.0	0.0	0.0	0.9885189632516199	0.0
4	0.0	0.0	0.0	1.421960668811455	0.0
5	0.0	0.0	0.0	2.6584235805881553	0.0
6	0.0	0.0	0.0	3.864796207349676	0.0
7	0.0	0.0	0.0	4.909743305088586	0.0
8	0.0	0.0	0.0	6.843026417146223	0.0
9	0.0	0.0	0.0	7.856679618469346	0.0
10	0.0	0.0	0.0	8.927964565680366	0.0
11	0.0	0.0	0.0	10.49931464950836	0.0
12	7.080067062395215E-5	0.0	0.0	11.405563233494947	0.0
13	1.416013412479043E-4	0.0	0.0	11.789232067606145	0.0
14	1.416013412479043E-4	0.0	0.0	12.011192170012235	0.0
15	1.416013412479043E-4	0.0	0.0	12.281579931125108	0.0
16	1.416013412479043E-4	0.0	0.0	12.750917576691286	0.0
17	1.416013412479043E-4	0.0	0.0	13.330491866418958	0.0
18	1.416013412479043E-4	0.0	0.0	13.922102270152703	0.0
19	2.1240201187185646E-4	0.0	0.0	14.315116792786261	0.0
20	2.1240201187185646E-4	0.0	0.0	14.78247201957497	0.0
21	2.1240201187185646E-4	0.0	0.0	15.248057229598079	0.0
22	2.1240201187185646E-4	0.0	0.0	15.752087203769994	0.0
23	2.1240201187185646E-4	0.0	0.0	16.240682631745887	0.0
24	4.248040237437129E-4	0.0	0.0	16.569976550817888	0.0
25	4.248040237437129E-4	0.0	0.0	16.947698128596674	0.0
26	4.248040237437129E-4	0.0	0.0	17.247963772712854	0.0
27	4.248040237437129E-4	0.0	0.0	17.653014409352487	0.0
28	4.248040237437129E-4	0.0	0.0	17.985494358602566	0.0
29	4.248040237437129E-4	0.0	0.0	18.41836965879741	0.0
30	4.248040237437129E-4	0.0	0.0	19.289288708142642	0.0
31	4.248040237437129E-4	0.0	0.0	19.705242648058363	0.0
32	4.248040237437129E-4	0.0	0.0	20.017190402827495	0.0
33	4.248040237437129E-4	0.0	0.0	20.38379627531832	0.0
34	4.248040237437129E-4	0.0	0.0	20.765128687298926	0.0
35	4.248040237437129E-4	0.0	0.0	21.178038198377816	0.0
36	4.248040237437129E-4	0.0	0.0	21.494587996737504	0.0
37	4.248040237437129E-4	0.0	0.0	21.893549775703477	0.0
38	4.248040237437129E-4	0.0	0.0	22.321752231637138	0.0
39	4.248040237437129E-4	0.0	0.0	23.546674634102136	0.0
40	4.248040237437129E-4	0.0	0.0	23.961920567311612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	70	0.0	46.000004	1
TCCCGAA	35	1.0197982E-7	46.000004	29
ACGTCCC	35	1.0197982E-7	46.000004	43
CTCGACC	35	1.0197982E-7	46.000004	33
TGTACGC	35	1.0197982E-7	46.000004	16
CGTCCCA	35	1.0197982E-7	46.000004	44
TCGCCTG	20	6.3119474E-4	46.0	42
CTGTCGA	25	3.417556E-5	46.0	42
TCGTTAG	50	1.6370905E-11	46.0	1
TCGTTAC	25	3.417556E-5	46.0	21
AGATCCG	30	1.8617084E-6	46.0	19
GTCGACA	20	6.3119474E-4	46.0	10
GTCGAAA	25	3.417556E-5	46.0	44
GGTCGAC	20	6.3119474E-4	46.0	9
CGACGAT	25	3.417556E-5	46.0	20
ACGTTCC	25	3.417556E-5	46.0	14
GACGTAC	20	6.3119474E-4	46.0	13
CCGGATA	20	6.3119474E-4	46.0	34
TCGGGTA	20	6.3119474E-4	46.0	26
ACATTCG	20	6.3119474E-4	46.0	17
>>END_MODULE