FastQCFastQC Report
Sat 14 Jan 2017
SRR2933105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279365
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC341692.6707780813137765No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG182961.430084455960574No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162271.2683636022558067No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA43460.3396997729342291No Hit
GGGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT41250.32242557831424185No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCG29560.23105212351439974No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT27350.21377792889441244TruSeq Adapter, Index 13 (95% over 23bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCATGTGCTTT25310.19783251847596267No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG25240.1972853720400355No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG24760.19353351076510614No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT21500.1680521196062109TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT20910.16344045678911023TruSeq Adapter, Index 19 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG18180.14210174578794948No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG17430.13623946254587238No Hit
AGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15200.11880894037276306No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCATGT15170.11857444904307995No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC14160.1106799076104161No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA14150.11060174383385507No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC14110.11028908872761096No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC13980.10927295963231759TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCA13030.1018474008590199No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG253.417374E-546.017
GATCGTA405.6115823E-946.09
CAACGGT206.311724E-446.014
ACGCATA206.311724E-446.019
CTATCGA206.311724E-446.020
TTTCGCG206.311724E-446.01
TTAGACG301.8615701E-646.01
GACCGTA206.311724E-446.018
CGCATAT301.8615701E-646.030
ATTACGT206.311724E-446.038
CCAATCG301.8615701E-646.043
CTAAGCG253.417374E-546.01
TCGATTG206.311724E-446.023
CCGCTAG301.8615701E-646.01
ATCGTAC301.8615701E-646.010
ATGTACG206.311724E-446.039
CGTTAGT253.417374E-546.016
TATTGCG650.046.01
ACGATTT253.417374E-546.015
GTTACCG301.8615701E-646.038
Produced by FastQC (version 0.11.5)