##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1279365
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45630293153244	33.0	31.0	34.0	31.0	34.0
2	32.84783779453088	34.0	31.0	34.0	31.0	34.0
3	32.8845489754683	34.0	31.0	34.0	31.0	34.0
4	36.33600731612949	37.0	37.0	37.0	35.0	37.0
5	36.13218823400671	37.0	35.0	37.0	35.0	37.0
6	36.22449105610987	37.0	36.0	37.0	35.0	37.0
7	36.490797387766584	37.0	37.0	37.0	35.0	37.0
8	36.458922981322765	37.0	37.0	37.0	35.0	37.0
9	38.27945347887429	39.0	39.0	39.0	37.0	39.0
10	38.000574503757726	39.0	38.0	39.0	35.0	39.0
11	37.65593634342037	39.0	37.0	39.0	35.0	39.0
12	37.18338316274089	39.0	35.0	39.0	35.0	39.0
13	37.03682842660226	39.0	35.0	39.0	34.0	39.0
14	37.57604358412181	40.0	36.0	41.0	31.0	41.0
15	37.982099713529756	40.0	35.0	41.0	33.0	41.0
16	38.266268031406206	40.0	35.0	41.0	33.0	41.0
17	38.35125081583442	40.0	35.0	41.0	34.0	41.0
18	38.32496199286365	40.0	36.0	41.0	35.0	41.0
19	38.26897171643745	40.0	36.0	41.0	35.0	41.0
20	38.06243018997706	40.0	35.0	41.0	33.0	41.0
21	38.024653636765116	40.0	35.0	41.0	34.0	41.0
22	37.942184599391105	40.0	35.0	41.0	34.0	41.0
23	37.94754741610095	40.0	35.0	41.0	34.0	41.0
24	37.92597030558129	40.0	35.0	41.0	34.0	41.0
25	37.726590144329414	40.0	35.0	41.0	34.0	41.0
26	37.64450332782279	39.0	35.0	41.0	34.0	41.0
27	37.54567617529008	39.0	35.0	41.0	34.0	41.0
28	37.535608680869025	39.0	35.0	41.0	34.0	41.0
29	37.4722139498892	39.0	35.0	41.0	34.0	41.0
30	37.43323680106928	39.0	35.0	41.0	34.0	41.0
31	37.29918436099159	40.0	35.0	41.0	33.0	41.0
32	37.17978997393238	39.0	35.0	41.0	33.0	41.0
33	37.11263869185103	39.0	35.0	41.0	33.0	41.0
34	36.9456894631321	39.0	35.0	41.0	33.0	41.0
35	36.86336659202026	39.0	35.0	41.0	32.0	41.0
36	36.82197340086684	39.0	35.0	41.0	32.0	41.0
37	36.718909771644526	39.0	35.0	41.0	32.0	41.0
38	36.7023570286822	39.0	35.0	41.0	32.0	41.0
39	36.675992386848165	39.0	35.0	41.0	32.0	41.0
40	36.61482532350033	39.0	35.0	41.0	32.0	41.0
41	36.45673674049235	39.0	35.0	41.0	31.0	41.0
42	36.50727665677895	39.0	35.0	41.0	32.0	41.0
43	36.48827582433473	39.0	35.0	41.0	32.0	41.0
44	36.4523853630512	39.0	35.0	41.0	32.0	41.0
45	36.39421978872331	38.0	35.0	41.0	32.0	41.0
46	36.35665036951925	38.0	35.0	41.0	32.0	41.0
47	36.2642248302869	38.0	35.0	41.0	32.0	41.0
48	36.14204077804223	38.0	35.0	41.0	31.0	41.0
49	36.136869462584954	38.0	35.0	41.0	31.0	41.0
50	36.0312225205473	38.0	35.0	41.0	31.0	41.0
51	35.96352800021886	37.0	35.0	41.0	31.0	41.0
52	35.71940298507463	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	13.0
15	34.0
16	76.0
17	155.0
18	295.0
19	540.0
20	964.0
21	1520.0
22	2410.0
23	3793.0
24	6167.0
25	8894.0
26	11400.0
27	12467.0
28	13369.0
29	14756.0
30	17324.0
31	21832.0
32	27390.0
33	39162.0
34	112670.0
35	147511.0
36	78732.0
37	88432.0
38	143031.0
39	522980.0
40	3440.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.976472703255126	21.579064614085894	25.4485623727396	12.995900309919376
2	39.732367229055036	22.27777061276493	25.43253879854459	12.557323359635445
3	36.023496031234245	22.340692452896555	28.423866527535147	13.211944988334057
4	32.45977496648728	25.804129392315716	27.88094093554224	13.85515470565476
5	26.120536359834762	29.577642033352486	29.787589937195406	14.51423166961735
6	27.054593489739048	36.04553821622446	27.79574241909072	9.104125874945773
7	86.53050536789736	1.7291390650830685	9.274601071625376	2.4657544953941994
8	85.18155491200713	1.7458661132671287	9.23067302919808	3.8419059455276643
9	79.1752939935046	3.9811937953594168	11.82047343799463	5.023038773141363
10	51.20047836231255	21.147209748586214	16.90190055222708	10.750411336874151
11	34.24401949404587	23.654000226674953	26.970332938606262	15.131647340672913
12	33.09884200365025	17.169611486948604	32.69895612276403	17.03259038663712
13	30.072027920100986	19.589952828160843	34.908411594814616	15.429607656923553
14	17.68002094789212	21.54959687032239	42.197965396896116	18.572416784889377
15	15.632755312205665	28.796238759071883	39.53758309786496	16.033422830857496
16	31.14787414068698	23.8219741825046	29.83941252105537	15.19073915575305
17	26.624067408440904	22.44214903487277	26.554110828418786	24.37967272826754
18	23.131944363023845	22.04234131776311	35.44156671473739	19.384147604475658
19	23.37167266573652	24.805587146748582	27.52115307203183	24.301587115483073
20	17.62538446807596	38.62259792944156	25.286763355258273	18.46525424722421
21	24.83192833944965	26.20854877224248	33.15801198250695	15.801510905800924
22	18.789321264846233	21.869990190446043	38.400534640231676	20.94015390447605
23	22.0900993852419	23.27146670418528	34.75450711876595	19.88392679180687
24	23.880049868489444	28.50695462201952	28.875027845845402	18.737967663645634
25	24.998104528418395	24.02777940618979	31.05118554908099	19.922930516310828
26	17.888640067533505	29.788449738737576	26.50283539099475	25.820074802734165
27	23.937578408038362	25.181867567113375	32.16290894310849	18.71764508173977
28	17.878166121474326	30.235468376890097	26.342443321491523	25.543922180144058
29	23.630003947270716	29.806740062452857	25.722057426926636	20.841198563349785
30	26.731620764988882	24.00433027322148	26.18494331172105	23.079105650068588
31	24.981533807787457	24.034970473633404	31.41456894631321	19.56892677226593
32	28.323504238430786	23.966108186483137	25.47810828027967	22.23227929480641
33	27.64809104516694	22.794589503386444	30.661773614253946	18.89554583719267
34	19.910111656954818	22.76543441472918	35.588436450895564	21.73601747742044
35	21.78385370867579	28.406670496691717	30.020986974006636	19.788488820625858
36	27.27822005448015	23.739433234456158	26.914992984801057	22.06735372626264
37	20.773352405294816	28.245027806763513	28.265037733563137	22.716582054378538
38	23.51783892790564	23.24043568489055	33.42884946829091	19.812875918912898
39	20.46741938383495	26.839955759302462	28.17733797626166	24.51528688060092
40	27.471128255032774	22.92512301024336	29.962911288021793	19.640837446702076
41	19.618795261711867	22.867672634471006	27.720158047156207	29.79337405666092
42	17.825874554955	26.312193939962402	32.96705787636836	22.894873628714247
43	18.339723222067196	27.475583590296747	32.16290894310849	22.02178424452756
44	25.026712470639733	24.06482903627972	28.285594806798684	22.622863686281868
45	19.747843656814123	23.59076573143708	26.4642224853736	30.197168126375196
46	23.373548596373983	22.861263204793005	29.012205273710006	24.75298292512301
47	18.66668229942198	22.887448069940948	32.895616184591574	25.550253446045502
48	19.081497461631354	29.30907129708879	29.34885665935835	22.2605745819215
49	24.76869384421178	24.61009954156945	29.531369077628355	21.089837536590416
50	21.685680005315135	24.81684273057337	28.74848069159309	24.748996572518397
51	20.705193592133597	25.682193900880513	27.511304436185142	26.101308070800748
52	20.716761831064627	26.03447804184107	28.847748687825604	24.4010114392687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	766.0
1	2170.5
2	3575.0
3	11737.0
4	19899.0
5	11741.5
6	3584.0
7	4052.0
8	4520.0
9	6085.0
10	7650.0
11	8964.0
12	10278.0
13	10603.5
14	10306.5
15	9684.0
16	8642.5
17	7601.0
18	6847.5
19	6094.0
20	6244.5
21	6395.0
22	6083.5
23	5772.0
24	5872.5
25	5973.0
26	7109.0
27	8245.0
28	9130.5
29	10016.0
30	11832.5
31	13649.0
32	14231.5
33	14814.0
34	18729.0
35	22644.0
36	23382.0
37	24120.0
38	25822.0
39	32545.0
40	37566.0
41	44449.5
42	51333.0
43	63303.0
44	75273.0
45	87292.5
46	99312.0
47	105771.0
48	112230.0
49	123846.5
50	135463.0
51	113575.5
52	91688.0
53	87291.0
54	82894.0
55	78775.0
56	74656.0
57	74071.5
58	73487.0
59	82702.0
60	91917.0
61	67360.5
62	42804.0
63	35195.0
64	22904.5
65	18223.0
66	16063.0
67	13903.0
68	11233.0
69	8563.0
70	7321.0
71	6079.0
72	5517.0
73	4955.0
74	4075.5
75	3196.0
76	2754.0
77	2312.0
78	1765.0
79	1218.0
80	837.5
81	457.0
82	354.5
83	252.0
84	204.5
85	157.0
86	112.5
87	68.0
88	45.5
89	15.0
90	7.0
91	8.0
92	9.0
93	5.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1279365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.31755738990689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32271594007891	14.287858169266734
2	11.261300771097188	4.576042494035397
3	4.798562541391604	2.9248520947134415
4	2.5570803855578186	2.078145099367049
5	1.5382099094148192	1.5626334056129538
6	1.046715886673898	1.276002605905452
7	0.7161415606607607	1.0185173080615748
8	0.5842145702067083	0.9495850446555669
9	0.4559212491965536	0.8336885531246119
>10	4.499561874761336	20.98883387990224
>50	1.5758057389809172	23.317340354637484
>100	0.6267672543522271	16.32363758233451
>500	0.006955493574808898	0.9174720142516501
>1k	0.008887575123366924	3.552358054838646
>5k	0.0	0.0
>10k+	0.0011592489291348163	5.393033339292688
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	34169	2.6707780813137765	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	18296	1.430084455960574	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16227	1.2683636022558067	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	4346	0.3396997729342291	No Hit
GGGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	4125	0.32242557831424185	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCG	2956	0.23105212351439974	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	2735	0.21377792889441244	TruSeq Adapter, Index 13 (95% over 23bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCATGTGCTTT	2531	0.19783251847596267	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2524	0.1972853720400355	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2476	0.19353351076510614	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2150	0.1680521196062109	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2091	0.16344045678911023	TruSeq Adapter, Index 19 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	1818	0.14210174578794948	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	1743	0.13623946254587238	No Hit
AGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1520	0.11880894037276306	No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCATGT	1517	0.11857444904307995	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1416	0.1106799076104161	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1415	0.11060174383385507	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1411	0.11028908872761096	No Hit
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1398	0.10927295963231759	TruSeq Adapter, Index 13 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCA	1303	0.1018474008590199	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2190930657005624	0.0
2	0.0	0.0	0.0	0.6570447057720041	0.0
3	0.0	0.0	0.0	1.0205062667807858	0.0
4	0.0	0.0	0.0	1.4566601399913237	0.0
5	0.0	0.0	0.0	2.8685324360131785	0.0
6	0.0	0.0	0.0	4.22920745838756	0.0
7	0.0	0.0	0.0	5.435743513383593	0.0
8	0.0	0.0	0.0	7.450102199137854	0.0
9	0.0	0.0	0.0	8.631000535421869	0.0
10	0.0	0.0	0.0	9.725449734829388	0.0
11	0.0	0.0	0.0	11.27004412345187	0.0
12	0.0	0.0	0.0	12.198551625220324	0.0
13	7.816377656102832E-5	0.0	0.0	12.64893130576497	0.0
14	7.816377656102832E-5	0.0	0.0	12.989725371571053	0.0
15	7.816377656102832E-5	0.0	0.0	13.27760256064532	0.0
16	7.816377656102832E-5	0.0	0.0	13.703829634232608	0.0
17	7.816377656102832E-5	0.0	0.0	14.164370605730186	0.0
18	7.816377656102832E-5	0.0	0.0	14.810316055230524	0.0
19	1.5632755312205664E-4	0.0	0.0	15.175809874429893	0.0
20	1.5632755312205664E-4	0.0	0.0	15.766102715018778	0.0
21	2.3449132968308498E-4	0.0	0.0	16.217029541999352	0.0
22	2.3449132968308498E-4	0.0	0.0	16.7025047582199	0.0
23	2.3449132968308498E-4	0.0	0.0	17.100592872245215	0.0
24	6.253102124882266E-4	0.0	0.0	17.418172296412674	0.0
25	6.253102124882266E-4	0.0	0.0	17.827359666709658	0.0
26	6.253102124882266E-4	0.0	0.0	18.148847279705166	0.0
27	6.253102124882266E-4	0.0	0.0	18.63486964236164	0.0
28	6.253102124882266E-4	0.0	0.0	19.000676116667254	0.0
29	6.253102124882266E-4	0.0	0.0	19.493576891661096	0.0
30	6.253102124882266E-4	0.0	0.0	21.27391323039164	0.0
31	7.034739890492549E-4	0.0	0.0	21.867410785819526	0.0
32	7.034739890492549E-4	0.0	0.0	22.23071601927519	0.0
33	7.034739890492549E-4	0.0	0.0	22.688990241252498	0.0
34	7.816377656102832E-4	0.0	0.0	23.19689846134606	0.0
35	7.816377656102832E-4	0.0	0.0	23.67901263517448	0.0
36	7.816377656102832E-4	0.0	0.0	23.995497766470084	0.0
37	7.816377656102832E-4	0.0	0.0	24.390928312092328	0.0
38	7.816377656102832E-4	0.0	0.0	24.95230055535363	0.0
39	7.816377656102832E-4	0.0	0.0	27.01261954172578	0.0
40	7.816377656102832E-4	0.0	0.0	27.43900294286619	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCCG	25	3.417374E-5	46.0	17
GATCGTA	40	5.6115823E-9	46.0	9
CAACGGT	20	6.311724E-4	46.0	14
ACGCATA	20	6.311724E-4	46.0	19
CTATCGA	20	6.311724E-4	46.0	20
TTTCGCG	20	6.311724E-4	46.0	1
TTAGACG	30	1.8615701E-6	46.0	1
GACCGTA	20	6.311724E-4	46.0	18
CGCATAT	30	1.8615701E-6	46.0	30
ATTACGT	20	6.311724E-4	46.0	38
CCAATCG	30	1.8615701E-6	46.0	43
CTAAGCG	25	3.417374E-5	46.0	1
TCGATTG	20	6.311724E-4	46.0	23
CCGCTAG	30	1.8615701E-6	46.0	1
ATCGTAC	30	1.8615701E-6	46.0	10
ATGTACG	20	6.311724E-4	46.0	39
CGTTAGT	25	3.417374E-5	46.0	16
TATTGCG	65	0.0	46.0	1
ACGATTT	25	3.417374E-5	46.0	15
GTTACCG	30	1.8615701E-6	46.0	38
>>END_MODULE