##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933104.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1193318
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.597587566767615	33.0	31.0	34.0	31.0	34.0
2	32.942640603761944	34.0	31.0	34.0	31.0	34.0
3	33.006353712924806	34.0	33.0	34.0	31.0	34.0
4	36.411295228933106	37.0	37.0	37.0	35.0	37.0
5	36.38412476808361	37.0	37.0	37.0	35.0	37.0
6	36.540182918551466	37.0	37.0	37.0	35.0	37.0
7	36.613595035020005	37.0	37.0	37.0	35.0	37.0
8	36.477407530934755	37.0	37.0	37.0	35.0	37.0
9	38.11695960339155	39.0	39.0	39.0	35.0	39.0
10	37.891406146559426	39.0	38.0	39.0	35.0	39.0
11	37.27440045319018	39.0	35.0	39.0	35.0	39.0
12	37.03903066910916	39.0	35.0	39.0	34.0	39.0
13	37.091403129760884	39.0	35.0	39.0	35.0	39.0
14	38.44373167923387	40.0	35.0	41.0	35.0	41.0
15	38.518623702986126	40.0	35.0	41.0	35.0	41.0
16	38.515994060258876	40.0	35.0	41.0	35.0	41.0
17	38.5327900861296	40.0	36.0	41.0	35.0	41.0
18	38.47872402829757	40.0	36.0	41.0	35.0	41.0
19	38.378511846800265	40.0	35.0	41.0	35.0	41.0
20	38.27453369512569	40.0	35.0	41.0	35.0	41.0
21	38.218003918486104	40.0	35.0	41.0	35.0	41.0
22	38.183857949012754	40.0	35.0	41.0	35.0	41.0
23	38.15577658260413	40.0	35.0	41.0	35.0	41.0
24	38.12197503096409	40.0	35.0	41.0	35.0	41.0
25	38.03928123098788	40.0	35.0	41.0	35.0	41.0
26	38.012740107833785	40.0	35.0	41.0	35.0	41.0
27	37.9932867852492	40.0	35.0	41.0	35.0	41.0
28	38.0273648767554	40.0	35.0	41.0	35.0	41.0
29	38.012051272167184	40.0	35.0	41.0	35.0	41.0
30	37.91615059858311	40.0	35.0	41.0	35.0	41.0
31	37.80514498230983	40.0	35.0	41.0	34.0	41.0
32	37.73667203545074	40.0	35.0	41.0	34.0	41.0
33	37.64135460958437	40.0	35.0	41.0	34.0	41.0
34	37.496312801784605	40.0	35.0	41.0	34.0	41.0
35	37.39847551113785	40.0	35.0	41.0	34.0	41.0
36	37.33546715963389	40.0	35.0	41.0	34.0	41.0
37	37.25504014856057	40.0	35.0	41.0	33.0	41.0
38	37.27554180863776	40.0	35.0	41.0	33.0	41.0
39	37.17728803219259	39.0	35.0	41.0	33.0	41.0
40	37.199945027226605	39.0	35.0	41.0	33.0	41.0
41	37.125513065251674	39.0	35.0	41.0	33.0	41.0
42	37.045380192035985	39.0	35.0	41.0	33.0	41.0
43	36.92874489448747	39.0	35.0	41.0	33.0	41.0
44	36.87921995645754	39.0	35.0	41.0	33.0	41.0
45	36.82776761936047	39.0	35.0	41.0	33.0	41.0
46	36.73690835133636	39.0	35.0	41.0	33.0	41.0
47	36.682945367454444	39.0	35.0	41.0	33.0	41.0
48	36.652350840262194	38.0	35.0	41.0	33.0	41.0
49	36.54142986194795	38.0	35.0	41.0	33.0	41.0
50	36.449395718492475	38.0	35.0	41.0	32.0	41.0
51	36.35124752999619	38.0	35.0	41.0	32.0	41.0
52	36.14113253969185	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	5.0
15	6.0
16	22.0
17	60.0
18	129.0
19	278.0
20	447.0
21	821.0
22	1276.0
23	2199.0
24	3611.0
25	5869.0
26	7962.0
27	9283.0
28	9559.0
29	10637.0
30	12365.0
31	15554.0
32	19585.0
33	27625.0
34	70676.0
35	210113.0
36	54788.0
37	71907.0
38	128438.0
39	525816.0
40	4285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.406785115116	22.381963567129635	25.017807491381173	20.19344382637319
2	25.49077446246516	25.19445780588242	28.069466814378064	21.245300917274356
3	25.196636604827884	24.94666132581592	27.720942783063695	22.135759286292505
4	24.500845541590756	29.55473729550715	24.862609966496777	21.081807196405318
5	22.644173640219957	36.54834671060019	21.758659468808816	19.04882018037103
6	89.62347002224051	2.5217921794525853	5.835242575742593	2.0194952225643124
7	91.96475708905757	1.5500478497768408	4.22770795378935	2.2574871073762397
8	85.466070234422	3.8722285258413938	7.181488924159361	3.4802123155772393
9	48.610093872714565	29.972061093522427	11.268245346169252	10.149599687593751
10	37.338747928046004	20.65903640102638	28.05069562346332	13.951520047464298
11	35.81652166480352	21.653406719751146	26.007066012580047	16.523005602865286
12	19.48927276719198	38.29825746364339	26.60380552375813	15.608664245406507
13	15.03664572226347	40.517615589474055	28.267318518617834	16.17842016964464
14	14.344877057079506	26.34762904774754	43.062285157853985	16.245208737318972
15	16.238672340482587	21.59206514944047	43.15899031104869	19.010272199028254
16	16.593313768836136	22.084557511074166	28.191647155242777	33.130481564846924
17	20.322914763709253	24.983952307767083	32.716509765209274	21.976623163314386
18	24.887498554450698	27.292724990321105	27.86767651204457	19.952099943183626
19	27.291803190767254	25.259151374570738	26.17190053280014	21.277144901861867
20	20.044028498690206	28.864812229430882	29.42392555882003	21.667233713058884
21	20.62635441684446	24.58154490253227	28.22047434129042	26.571626339332855
22	17.15904729502111	29.60384407173947	26.41684781424566	26.820260818993763
23	17.7328256173124	25.26568777140712	38.3588448343191	18.64264177696138
24	17.457207550711544	25.449125882623076	34.95740448061623	22.13626208604915
25	15.799560553012693	35.773867485448136	27.469626704700673	20.956945256838498
26	17.05488394543617	32.176083826775425	30.01706167174215	20.751970556046253
27	17.087230729780327	28.369219269297872	36.71309743086084	17.830452570060956
28	15.62852483579398	24.79188280072872	35.86805863985962	23.711533723617677
29	19.754583438781616	28.727128896069615	31.5414667339301	19.97682093121867
30	28.620786747539213	25.983015424220536	25.628457795826424	19.767740032413826
31	26.531737558639023	27.123113872412887	27.79736834607372	18.547780222874373
32	25.005740297221696	27.540856670225374	27.292138390605018	20.161264641947913
33	19.61941410420357	26.271706284494158	30.17929839321958	23.929581218082692
34	20.564510046777137	24.50411374000895	31.856889781265345	23.074486431948568
35	26.860400999565915	22.82006975508624	31.069505362359408	19.25002388298844
36	20.173667035945154	28.538076187571125	30.673718153920415	20.614538622563305
37	20.118526662633094	29.2206268572166	29.50889871769302	21.151947762457283
38	19.777628427627842	28.195753353255377	30.252288157892533	21.77433006122425
39	23.27493593493101	25.155993624499086	28.084550807077406	23.4845196334925
40	18.96887501906449	22.637888643261896	31.104366145486782	27.288870192186828
41	23.292952926210784	25.391387710568345	25.372281319815844	25.943378043405023
42	22.71464940610969	25.064651668708592	28.23589353382753	23.984805391354193
43	20.869458099182282	28.402823053033643	29.876193939922135	20.851524907861947
44	18.697530750395117	32.852014299625075	25.805024310368236	22.645430639611572
45	22.51947930057202	28.694195512009372	27.778513355199536	21.007811832219073
46	22.695878215194945	26.057010788406775	28.314665495701902	22.932445500696378
47	22.185033662443708	22.669397428011646	32.8869588827119	22.25861002683275
48	20.072436684940644	22.077685914400018	32.46385288749521	25.386024513164134
49	20.006234716982398	28.466427222249223	30.860675863432885	20.666662197335498
50	18.66744656495586	28.81143165526708	29.556413294695965	22.964708485081093
51	21.421867431816164	22.978870678226592	31.075455159479702	24.523806730477542
52	23.608878773302674	23.078341230082845	30.521537427575886	22.791242569038598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1868.0
1	1585.5
2	1303.0
3	4008.5
4	6714.0
5	4404.5
6	2095.0
7	2320.5
8	2546.0
9	2923.5
10	3301.0
11	3553.5
12	3806.0
13	3802.0
14	3716.0
15	3634.0
16	3534.0
17	3434.0
18	3521.5
19	3609.0
20	3395.5
21	3182.0
22	3713.0
23	4244.0
24	4180.0
25	4116.0
26	5831.5
27	7547.0
28	9094.0
29	10641.0
30	12771.0
31	14901.0
32	16421.5
33	17942.0
34	22351.5
35	26761.0
36	29379.5
37	31998.0
38	35649.0
39	42812.5
40	46325.0
41	63622.5
42	80920.0
43	100710.0
44	120500.0
45	127757.5
46	135015.0
47	134627.0
48	134239.0
49	129903.0
50	125567.0
51	111987.5
52	98408.0
53	87188.0
54	75968.0
55	65753.0
56	55538.0
57	47531.0
58	39524.0
59	35880.5
60	32237.0
61	26389.5
62	20542.0
63	17776.5
64	10950.5
65	6890.0
66	5599.0
67	4308.0
68	3387.0
69	2466.0
70	1970.0
71	1474.0
72	1069.0
73	664.0
74	524.0
75	384.0
76	368.5
77	353.0
78	231.5
79	110.0
80	110.5
81	111.0
82	61.0
83	11.0
84	8.5
85	6.0
86	6.0
87	6.0
88	3.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1193318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.906401164380625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11311354891636	13.956997651884995
2	12.456483176025381	4.959275023986385
3	5.134204767410142	3.0661061928042543
4	2.5003040518583126	1.9908822195687161
5	1.4825288124514158	1.4755906639205343
6	0.9525701662529165	1.137734631999078
7	0.6166330998279191	0.8592462099487087
8	0.4777317583005605	0.7607936023756704
9	0.35153170915203574	0.6297958101942714
>10	3.467354596126763	16.064913320257833
>50	1.2949143373408551	18.66909740394352
>100	1.1420747567738252	33.867239367459426
>500	0.007177549303199641	1.022930852201343
>1k	0.003377670260329243	1.5393970494552456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4803	0.4024912051942567	No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCTT	3202	0.2683274701295044	TruSeq Adapter, Index 10 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	3008	0.2520702779979855	RNA PCR Primer, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	2266	0.18989070809289726	RNA PCR Primer, Index 35 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1678	0.14061633194169534	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2786348651407253	0.0
2	0.0	0.0	0.0	0.8669943803747199	0.0
3	0.0	0.0	0.0	1.4577002944730575	0.0
4	0.0	0.0	0.0	1.8054701261524588	0.0
5	0.0	0.0	0.0	3.4638713234862792	0.0
6	0.0	0.0	0.0	3.7701601752424754	0.0
7	0.0	0.0	0.0	4.683495933187968	0.0
8	8.379995944081963E-5	0.0	0.0	5.944852922691186	0.0
9	8.379995944081963E-5	0.0	0.0	6.81335570233584	0.0
10	8.379995944081963E-5	0.0	0.0	7.576186733125621	0.0
11	8.379995944081963E-5	0.0	0.0	8.370023748908505	0.0
12	8.379995944081963E-5	0.0	0.0	8.982266252583134	0.0
13	8.379995944081963E-5	0.0	0.0	9.213386540720915	0.0
14	8.379995944081963E-5	0.0	0.0	9.376712661671071	0.0
15	8.379995944081963E-5	0.0	0.0	9.958954779865886	0.0
16	8.379995944081963E-5	0.0	0.0	10.769886987374699	0.0
17	8.379995944081963E-5	0.0	0.0	11.732999921228037	0.0
18	8.379995944081963E-5	0.0	0.0	12.214179288337224	0.0
19	8.379995944081963E-5	0.0	0.0	12.689827858123317	0.0
20	8.379995944081963E-5	0.0	0.0	13.276259974290172	0.0
21	8.379995944081963E-5	0.0	0.0	13.869396087212294	0.0
22	8.379995944081963E-5	0.0	0.0	14.52739336874161	0.0
23	8.379995944081963E-5	0.0	0.0	15.000360339825596	0.0
24	8.379995944081963E-5	0.0	0.0	15.387097152644978	0.0
25	8.379995944081963E-5	0.0	0.0	15.772493166113307	0.0
26	8.379995944081963E-5	0.0	0.0	16.12462059568363	0.0
27	8.379995944081963E-5	0.0	0.0	16.53565939674085	0.0
28	8.379995944081963E-5	0.0	0.0	16.92599960781619	0.0
29	8.379995944081963E-5	0.0	0.0	17.418240569571566	0.0
30	8.379995944081963E-5	0.0	0.0	17.87369334913242	0.0
31	8.379995944081963E-5	0.0	0.0	18.267972158301475	0.0
32	8.379995944081963E-5	0.0	0.0	18.655211770877504	0.0
33	8.379995944081963E-5	0.0	0.0	19.054183377775246	0.0
34	1.6759991888163925E-4	0.0	0.0	19.556396534704078	0.0
35	1.6759991888163925E-4	0.0	0.0	19.969027534990673	0.0
36	1.6759991888163925E-4	0.0	0.0	20.384507733898257	0.0
37	1.6759991888163925E-4	0.0	0.0	20.788507338362447	0.0
38	1.6759991888163925E-4	0.0	0.0	21.177925749883936	0.0
39	1.6759991888163925E-4	0.0	0.0	21.61686993743495	0.0
40	1.6759991888163925E-4	0.0	0.0	22.036875334152338	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAG	35	1.0196163E-7	46.000004	43
CTAAGCG	55	1.8189894E-12	46.000004	29
ACGATAT	70	0.0	46.000004	22
GTTCGAC	35	1.0196163E-7	46.000004	20
TTCGCGG	55	1.8189894E-12	46.000004	1
TCGCCGT	20	6.311552E-4	46.0	24
TCGCCCA	20	6.311552E-4	46.0	37
AACCGTA	25	3.417235E-5	46.0	9
TCGTTTG	20	6.311552E-4	46.0	10
CGGAAAG	30	1.8614646E-6	46.0	28
GTACCGT	25	3.417235E-5	46.0	40
AGTCCGA	30	1.8614646E-6	46.0	10
GTACCGC	20	6.311552E-4	46.0	27
ACCGGAT	65	0.0	46.0	17
CTCGTTG	25	3.417235E-5	46.0	15
GTATTAC	25	3.417235E-5	46.0	40
GTCGAAC	25	3.417235E-5	46.0	14
GGTCGCA	30	1.8614646E-6	46.0	43
ATAGGCG	20	6.311552E-4	46.0	2
CGCGGCC	20	6.311552E-4	46.0	35
>>END_MODULE