FastQCFastQC Report
Sat 14 Jan 2017
SRR2933103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242688
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290472.337433048359685No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC103050.8292507853942421No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC72320.581964258124324No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC60150.4840313900190555No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31300.2518733583972807No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC27390.22040930627800379No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT26920.21662718236596795RNA PCR Primer, Index 34 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25340.2039128083638049No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC24030.19337114384302417RNA PCR Primer, Index 34 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT23500.18910619560179226RNA PCR Primer, Index 34 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC22440.1805762991193284RNA PCR Primer, Index 34 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT21530.17325346345985476RNA PCR Primer, Index 34 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT20940.1685056908894268TruSeq Adapter, Index 3 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG18330.14750283256939795No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT17780.1430769428851007TruSeq Adapter, Index 18 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13800.11104959571509503No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA206.311654E-446.041
GCGACTA206.311654E-446.08
CCTAGCG206.311654E-446.032
TTTCGAT206.311654E-446.038
CGTTGGC253.417318E-546.041
AGCTTCG206.311654E-446.026
TTATCGT253.417318E-546.044
GCGTTAG206.311654E-446.011
CGCCTAT301.8615265E-646.036
CACTCGA301.8615265E-646.037
CCGTAAG206.311654E-446.028
TGTTCGG253.417318E-546.01
CTACCCG206.311654E-446.033
TTACGAG253.417318E-546.040
CGGTCGA206.311654E-446.010
CGCGTTC206.311654E-446.028
ATCGGAC253.417318E-546.016
TTCGCGA253.417318E-546.014
CGTCCCA206.311654E-446.013
TCGACGA206.311654E-446.029
Produced by FastQC (version 0.11.5)