##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933103.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242688
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.506356382293866	33.0	31.0	34.0	31.0	34.0
2	32.85503119045167	34.0	31.0	34.0	31.0	34.0
3	32.895169986352165	34.0	31.0	34.0	31.0	34.0
4	36.35148725987537	37.0	37.0	37.0	35.0	37.0
5	36.3386626409847	37.0	37.0	37.0	35.0	37.0
6	36.48148368697533	37.0	37.0	37.0	35.0	37.0
7	36.545839341813874	37.0	37.0	37.0	35.0	37.0
8	36.39211934129886	37.0	37.0	37.0	35.0	37.0
9	38.028472955399906	39.0	39.0	39.0	35.0	39.0
10	37.9063441507442	39.0	38.0	39.0	35.0	39.0
11	37.53545700932173	39.0	37.0	39.0	35.0	39.0
12	37.388690483854354	39.0	37.0	39.0	35.0	39.0
13	37.248506463408354	39.0	37.0	39.0	34.0	39.0
14	38.74375305788742	41.0	38.0	41.0	35.0	41.0
15	38.85161923185868	41.0	38.0	41.0	35.0	41.0
16	38.872836142297984	41.0	38.0	41.0	35.0	41.0
17	38.886075990111756	41.0	38.0	41.0	35.0	41.0
18	38.77738096770871	40.0	38.0	41.0	35.0	41.0
19	38.62458959932018	40.0	37.0	41.0	35.0	41.0
20	38.49850968223721	40.0	35.0	41.0	35.0	41.0
21	38.465761317402276	40.0	35.0	41.0	35.0	41.0
22	38.433676836019984	40.0	35.0	41.0	35.0	41.0
23	38.3582307063398	40.0	35.0	41.0	35.0	41.0
24	38.26059397048978	40.0	35.0	41.0	35.0	41.0
25	38.18382570685482	40.0	35.0	41.0	35.0	41.0
26	38.0873236081784	40.0	35.0	41.0	35.0	41.0
27	38.0110574818458	40.0	35.0	41.0	35.0	41.0
28	37.944257931194315	40.0	35.0	41.0	34.0	41.0
29	37.89172825359221	40.0	35.0	41.0	34.0	41.0
30	37.74108303934696	40.0	35.0	41.0	34.0	41.0
31	37.65049795282484	40.0	35.0	41.0	34.0	41.0
32	37.52862182623474	40.0	35.0	41.0	34.0	41.0
33	37.34689962404079	40.0	35.0	41.0	33.0	41.0
34	37.20206198176855	40.0	35.0	41.0	33.0	41.0
35	37.06623142735747	40.0	35.0	41.0	33.0	41.0
36	36.99042237472318	39.0	35.0	41.0	33.0	41.0
37	36.913583296853275	39.0	35.0	41.0	33.0	41.0
38	36.90317601843745	39.0	35.0	41.0	33.0	41.0
39	36.81445463382603	39.0	35.0	41.0	33.0	41.0
40	36.75542292192409	39.0	35.0	41.0	33.0	41.0
41	36.724660574496575	39.0	35.0	41.0	33.0	41.0
42	36.691413291188134	39.0	35.0	41.0	33.0	41.0
43	36.60388206854818	39.0	35.0	41.0	33.0	41.0
44	36.51004194134006	39.0	35.0	41.0	32.0	41.0
45	36.45368749034351	38.0	35.0	41.0	32.0	41.0
46	36.36189775712005	38.0	35.0	41.0	32.0	41.0
47	36.265839052119276	38.0	35.0	41.0	32.0	41.0
48	36.20585778570325	38.0	35.0	41.0	31.0	41.0
49	36.1285230081887	38.0	35.0	41.0	31.0	41.0
50	36.03079292630169	37.0	35.0	41.0	31.0	41.0
51	35.91878975253644	37.0	35.0	40.0	31.0	41.0
52	35.70697954756142	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	5.0
14	4.0
15	20.0
16	36.0
17	80.0
18	216.0
19	358.0
20	687.0
21	1102.0
22	1745.0
23	2858.0
24	4727.0
25	7754.0
26	10729.0
27	11950.0
28	12138.0
29	13068.0
30	14688.0
31	17827.0
32	22649.0
33	31314.0
34	73969.0
35	142766.0
36	105280.0
37	101362.0
38	142293.0
39	515902.0
40	7157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.393981433795126	21.822291677396098	24.03459275377247	20.74913413503631
2	30.862533475820157	23.351798681567697	25.342161507956945	20.443506334655197
3	26.649488849976827	23.526339676572075	29.176108564659835	20.648062908791264
4	22.79558505433383	27.35658508008446	27.293496034402843	22.554333831178862
5	21.60300896121955	36.9109543183808	24.041432765102744	17.444603955296905
6	84.85991656795592	2.6678458309728588	10.12273394448164	2.3495036565895866
7	86.53459275377247	1.9978466034917857	8.230947751969923	3.2366128907658234
8	79.02844479064737	4.330049054951846	12.138525518875213	4.502980635525571
9	38.967705490034504	28.62238952979348	17.594279497347685	14.815625482824329
10	30.444809960344028	22.7297600041201	28.233957356955248	18.591472678580626
11	29.85254544986352	21.012031982283567	30.935198537364165	18.200224030488744
12	20.936148091878252	28.60822668280373	32.75230790029356	17.70331732502446
13	15.917350131328217	34.2602487510944	32.15304243703971	17.669358680537673
14	14.174998068702681	30.880961271051138	37.51834732450945	17.425693335736725
15	17.19804166452078	23.87783578822681	39.94936782201164	18.974754725240768
16	17.15321947262708	26.80503875469949	29.189708116598855	26.852033656074575
17	19.04001648040377	30.11415576556626	31.54774166967091	19.298086084359067
18	21.501615852088378	34.19627452747592	26.97394679919658	17.328162821239122
19	20.56381006334655	29.60364950816295	27.032368543029307	22.800171885461193
20	19.441082556522634	30.94453314106196	29.744714683009732	19.869669619405673
21	16.696065303599937	28.09924936910954	29.379779960859043	25.824905366431476
22	14.561257532059535	33.4224680692177	28.498062265025492	23.518212133697276
23	14.872598753669465	33.61471262295927	33.08400821445125	18.428680408920016
24	20.896315084719575	30.558997914198898	28.905324586702374	19.639362414379153
25	16.09301771643405	32.55282098161405	26.302177215841787	25.051984086110107
26	14.887646778596075	31.727754673739504	31.19737214811763	22.187226399546788
27	17.53038574445074	29.351615208322603	33.630806767265796	19.48719227996086
28	16.35140920327548	32.51234420868311	28.7056767265798	22.430569861461606
29	21.023458824741205	30.067241334912705	29.95772068290673	18.951579157439358
30	24.845979038986453	29.515373126641602	26.675159010145748	18.963488824226193
31	24.84799080702477	31.384547046402638	24.5175780244116	19.249884122160992
32	23.715284930730803	30.206375212442705	23.499864809187827	22.578475047638666
33	18.654964077869906	28.091363238399342	27.77366482978833	25.48000785394242
34	19.56468558479683	30.572436524694858	26.45853182777978	23.404346062728536
35	26.236271694906527	31.3970200082402	23.81627568625431	18.55043261059896
36	19.340976978935984	33.29427820981614	24.70193644744296	22.662808363804913
37	18.837310732862957	34.97748429211516	26.402685147036102	19.782519827985787
38	16.926452979347996	32.73500669516403	24.72688237111809	25.611657954369882
39	21.59552518411701	29.48487472318072	28.56139272287171	20.35820736983056
40	16.37152688365865	28.32191185559046	31.172345753720965	24.13421550702992
41	18.269267909563787	33.02341376113715	25.213166941340067	23.494151387959008
42	19.060938868002268	28.64701357058248	28.459355847968276	23.83269171344698
43	20.950632821754134	29.32683022609054	30.020165962816087	19.702370989339236
44	19.72884585672349	32.57543325436473	24.722697893598394	22.973022995313386
45	21.18287132409744	30.820044934850905	26.395201369933563	21.60188237111809
46	21.971725807282276	29.96512398928774	27.391750785394244	20.67139941803574
47	18.13383568522429	32.129705927795236	28.72724287995056	21.00921550702992
48	18.96815612607509	29.98073530926508	27.24770819385075	23.803400370809086
49	17.75900306432508	29.5367783385693	31.203326981511047	21.500891615594583
50	17.272477081938508	29.85335015707885	30.81360727712829	22.060565483854354
51	17.37387019106968	27.96993291960653	29.290698872122366	25.36549801720142
52	18.37363843539167	28.03447043827574	28.85897345109955	24.732917675233043
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2783.0
1	2453.0
2	2123.0
3	17289.5
4	32456.0
5	18458.5
6	4461.0
7	5636.5
8	6812.0
9	9160.0
10	11508.0
11	14070.0
12	16632.0
13	17342.0
14	16721.0
15	15390.0
16	13222.0
17	11054.0
18	10210.0
19	9366.0
20	8645.5
21	7925.0
22	7302.0
23	6679.0
24	6963.0
25	7247.0
26	8127.5
27	9008.0
28	10558.0
29	12108.0
30	13372.0
31	14636.0
32	16439.0
33	18242.0
34	22170.0
35	26098.0
36	29993.5
37	33889.0
38	35816.5
39	38673.5
40	39603.0
41	45911.5
42	52220.0
43	62725.0
44	73230.0
45	77592.5
46	81955.0
47	90120.5
48	98286.0
49	117293.5
50	136301.0
51	131673.0
52	127045.0
53	109850.0
54	92655.0
55	79782.5
56	66910.0
57	59723.0
58	52536.0
59	45144.5
60	37753.0
61	32455.5
62	27158.0
63	23392.5
64	15903.0
65	12179.0
66	9581.0
67	6983.0
68	6015.5
69	5048.0
70	4180.5
71	3313.0
72	3004.5
73	2696.0
74	2067.5
75	1439.0
76	1061.0
77	683.0
78	581.0
79	479.0
80	372.5
81	266.0
82	158.0
83	50.0
84	46.0
85	42.0
86	25.0
87	8.0
88	6.5
89	3.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1242688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.319682589862573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50929871584403	18.359124282987008
2	11.058947389229964	5.6001807534658425
3	4.4401356619827475	3.372684768519975
4	2.3729154339276652	2.4032586239853804
5	1.4410957911944007	1.8244044007314548
6	0.9678895121478943	1.470399313778497
7	0.6759066803910849	1.1979619825508971
8	0.537065010939132	1.0878652485679914
9	0.42723865419489226	0.9735792402901258
>10	4.059209594600611	22.995636864838858
>50	1.149091022585346	20.982166510459553
>100	0.35034798113138244	12.087763634469445
>500	0.004151799229430073	0.737553799132797
>1k	0.005429275915408558	2.6540939365351015
>5k	6.387383429892421E-4	1.071195612006596
>10k+	6.387383429892421E-4	3.182131027680498
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29047	2.337433048359685	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10305	0.8292507853942421	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7232	0.581964258124324	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6015	0.4840313900190555	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3130	0.2518733583972807	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2739	0.22040930627800379	No Hit
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT	2692	0.21662718236596795	RNA PCR Primer, Index 34 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2534	0.2039128083638049	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	2403	0.19337114384302417	RNA PCR Primer, Index 34 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	2350	0.18910619560179226	RNA PCR Primer, Index 34 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2244	0.1805762991193284	RNA PCR Primer, Index 34 (96% over 25bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTT	2153	0.17325346345985476	RNA PCR Primer, Index 34 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	2094	0.1685056908894268	TruSeq Adapter, Index 3 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	1833	0.14750283256939795	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	1778	0.1430769428851007	TruSeq Adapter, Index 18 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1380	0.11104959571509503	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22419143019003965	0.0
2	0.0	0.0	0.0	0.6666999278982335	0.0
3	0.0	0.0	0.0	1.2696670443425864	0.0
4	0.0	0.0	0.0	1.6754004223103467	0.0
5	0.0	0.0	0.0	3.736175130040686	0.0
6	0.0	0.0	0.0	4.556332723901735	0.0
7	0.0	0.0	0.0	6.142008291703147	0.0
8	0.0	0.0	0.0	7.957991064531081	0.0
9	0.0	0.0	0.0	9.38280565998867	0.0
10	0.0	0.0	0.0	10.40285252613689	0.0
11	0.0	0.0	0.0	11.37775531750528	0.0
12	0.0	0.0	0.0	12.02095779471597	0.0
13	8.047072153267755E-5	0.0	0.0	12.326746536540146	0.0
14	8.047072153267755E-5	0.0	0.0	12.516013673585002	0.0
15	8.047072153267755E-5	0.0	0.0	13.017748622341248	0.0
16	8.047072153267755E-5	0.0	0.0	13.691208090848226	0.0
17	8.047072153267755E-5	0.0	0.0	14.48923623628779	0.0
18	8.047072153267755E-5	0.0	0.0	14.883381830354844	0.0
19	8.047072153267755E-5	0.0	0.0	15.258214451254055	0.0
20	8.047072153267755E-5	0.0	0.0	15.71577097388886	0.0
21	8.047072153267755E-5	0.0	0.0	16.190467760210126	0.0
22	8.047072153267755E-5	0.0	0.0	16.69461683061235	0.0
23	8.047072153267755E-5	0.0	0.0	17.058505433383118	0.0
24	8.047072153267755E-5	0.0	0.0	17.346349204305504	0.0
25	1.609414430653551E-4	0.0	0.0	17.621317659782665	0.0
26	1.609414430653551E-4	0.0	0.0	17.947626435597673	0.0
27	1.609414430653551E-4	0.0	0.0	18.291477828706803	0.0
28	1.609414430653551E-4	0.0	0.0	18.651986661173197	0.0
29	1.609414430653551E-4	0.0	0.0	18.9871472163568	0.0
30	1.609414430653551E-4	0.0	0.0	19.30782304166452	0.0
31	1.609414430653551E-4	0.0	0.0	19.572652186228563	0.0
32	1.609414430653551E-4	0.0	0.0	19.907490858526035	0.0
33	1.609414430653551E-4	0.0	0.0	20.25842367513004	0.0
34	1.609414430653551E-4	0.0	0.0	20.714692666220323	0.0
35	1.609414430653551E-4	0.0	0.0	21.01331951382809	0.0
36	1.609414430653551E-4	0.0	0.0	21.31419954163877	0.0
37	1.609414430653551E-4	0.0	0.0	21.692653345006953	0.0
38	1.609414430653551E-4	0.0	0.0	22.435719987639697	0.0
39	1.609414430653551E-4	0.0	0.0	22.82680769428851	0.0
40	1.609414430653551E-4	0.0	0.0	23.189811119122417	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	6.311654E-4	46.0	41
GCGACTA	20	6.311654E-4	46.0	8
CCTAGCG	20	6.311654E-4	46.0	32
TTTCGAT	20	6.311654E-4	46.0	38
CGTTGGC	25	3.417318E-5	46.0	41
AGCTTCG	20	6.311654E-4	46.0	26
TTATCGT	25	3.417318E-5	46.0	44
GCGTTAG	20	6.311654E-4	46.0	11
CGCCTAT	30	1.8615265E-6	46.0	36
CACTCGA	30	1.8615265E-6	46.0	37
CCGTAAG	20	6.311654E-4	46.0	28
TGTTCGG	25	3.417318E-5	46.0	1
CTACCCG	20	6.311654E-4	46.0	33
TTACGAG	25	3.417318E-5	46.0	40
CGGTCGA	20	6.311654E-4	46.0	10
CGCGTTC	20	6.311654E-4	46.0	28
ATCGGAC	25	3.417318E-5	46.0	16
TTCGCGA	25	3.417318E-5	46.0	14
CGTCCCA	20	6.311654E-4	46.0	13
TCGACGA	20	6.311654E-4	46.0	29
>>END_MODULE