FastQCFastQC Report
Sat 14 Jan 2017
SRR2933102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2909804
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379321.3035929567764701No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC154800.5319945948249435No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC110300.3790633321007188No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC101680.34943934368088025No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC50560.17375740771543377No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC40940.14069676170628675No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA29530.10148449861227768No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG206.313056E-446.016
CGTTTTT144100.043.2546841
CCCGCAC67550.041.74389332
CCGCACT66700.041.65517433
CGCACTT66750.041.5895134
ATAGCGG4450.041.3483161
CTAGCGG6900.041.3333321
TCACGAC4300.041.18604724
CGGTCTA4250.041.12941430
ACCCGCA68350.041.05340231
TACGCGG3700.041.0270271
CACTTAC68000.040.99411836
ATCAACG73650.040.5974214
TATGCGG5100.040.588231
CGCAAGC73250.040.47371719
AACGCAA73350.040.44989417
GCACTTA68750.040.4130935
TCAACGC73650.040.41004615
ACGCAAG73700.040.3514218
CAACGCA73750.040.3240716
Produced by FastQC (version 0.11.5)