##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933102.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2909804
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55576183138108	33.0	31.0	34.0	31.0	34.0
2	32.92431139691883	34.0	31.0	34.0	31.0	34.0
3	32.99420064031804	34.0	33.0	34.0	31.0	34.0
4	36.429748532890876	37.0	37.0	37.0	35.0	37.0
5	36.40900143102422	37.0	37.0	37.0	35.0	37.0
6	36.52521716239307	37.0	37.0	37.0	35.0	37.0
7	36.55438785567688	37.0	37.0	37.0	35.0	37.0
8	36.41993309515005	37.0	37.0	37.0	35.0	37.0
9	38.08679038175767	39.0	39.0	39.0	35.0	39.0
10	38.01407654948581	39.0	39.0	39.0	35.0	39.0
11	37.699699704859846	39.0	37.0	39.0	35.0	39.0
12	37.5770825113994	39.0	37.0	39.0	35.0	39.0
13	37.384113156762446	39.0	37.0	39.0	35.0	39.0
14	38.917414368802845	41.0	38.0	41.0	35.0	41.0
15	39.00906143506573	41.0	38.0	41.0	35.0	41.0
16	38.99833287740343	41.0	38.0	41.0	35.0	41.0
17	38.998404359881285	41.0	38.0	41.0	35.0	41.0
18	38.88218965950971	40.0	38.0	41.0	35.0	41.0
19	38.736331725435804	40.0	37.0	41.0	35.0	41.0
20	38.59386714706557	40.0	37.0	41.0	35.0	41.0
21	38.57086078649971	40.0	36.0	41.0	35.0	41.0
22	38.521749918551215	40.0	36.0	41.0	35.0	41.0
23	38.459571503785135	40.0	36.0	41.0	35.0	41.0
24	38.39379078453394	40.0	36.0	41.0	35.0	41.0
25	38.33185224846759	40.0	35.0	41.0	35.0	41.0
26	38.23017426603304	40.0	35.0	41.0	35.0	41.0
27	38.153127839538335	40.0	35.0	41.0	34.0	41.0
28	38.13118443716484	40.0	35.0	41.0	34.0	41.0
29	38.07163815844641	40.0	35.0	41.0	34.0	41.0
30	37.97144240643012	40.0	35.0	41.0	34.0	41.0
31	37.883737873753695	40.0	35.0	41.0	34.0	41.0
32	37.72570351817511	40.0	35.0	41.0	34.0	41.0
33	37.47238439427535	40.0	35.0	41.0	33.0	41.0
34	37.296092795253564	40.0	35.0	41.0	33.0	41.0
35	37.144712152433634	40.0	35.0	41.0	33.0	41.0
36	37.04087904202483	40.0	35.0	41.0	33.0	41.0
37	36.90996197682043	40.0	35.0	41.0	32.0	41.0
38	36.847611385509126	40.0	35.0	41.0	32.0	41.0
39	36.745385599854835	40.0	35.0	41.0	32.0	41.0
40	36.65770031246091	39.0	35.0	41.0	32.0	41.0
41	36.62280689695938	39.0	35.0	41.0	32.0	41.0
42	36.58203954630621	39.0	35.0	41.0	32.0	41.0
43	36.500113066034686	39.0	35.0	41.0	31.0	41.0
44	36.396600595778956	39.0	35.0	41.0	31.0	41.0
45	36.33815645314942	39.0	35.0	41.0	31.0	41.0
46	36.24924084233852	39.0	35.0	41.0	31.0	41.0
47	36.1617596924054	38.0	35.0	41.0	31.0	41.0
48	36.05431637319902	38.0	35.0	41.0	31.0	41.0
49	35.982408780797606	38.0	35.0	41.0	31.0	41.0
50	35.87773472027669	38.0	35.0	41.0	31.0	41.0
51	35.76891227037972	37.0	35.0	40.0	30.0	41.0
52	35.51911846983508	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	7.0
13	8.0
14	29.0
15	50.0
16	129.0
17	278.0
18	555.0
19	1058.0
20	1756.0
21	2887.0
22	4507.0
23	7434.0
24	12739.0
25	21650.0
26	30214.0
27	33938.0
28	34359.0
29	33756.0
30	35856.0
31	41396.0
32	51156.0
33	69261.0
34	171892.0
35	246000.0
36	184662.0
37	244143.0
38	411536.0
39	1256490.0
40	12052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.10013045552209	20.632420602899714	23.111178622340198	21.156270319237997
2	31.40208068997087	22.97824870678575	24.92009771104858	20.6995728921948
3	28.353387375919475	23.76826755341597	27.213138754362838	20.665206316301717
4	24.06258290936434	26.825208845681704	25.36280794170329	23.749400303250663
5	23.157023634581574	37.04276989103047	23.255758807122405	16.544447667265562
6	86.89107582503839	2.6007937304368265	8.557724162864577	1.9504062816602081
7	88.01022336899668	2.087528919473614	7.6416487158585245	2.2605989956711863
8	81.55126599592275	4.503774137364578	10.357948507872008	3.5870113588406647
9	37.23154549241117	31.968854259599617	16.365397806862592	14.43420244112662
10	31.59852003777574	23.08846231567487	26.725958174502473	18.587059472046917
11	29.01872428520959	20.892025717196073	31.065528812249898	19.023721185344442
12	24.90494205107973	23.163175251666434	33.39300516460902	18.53887753264481
13	19.8801362566001	26.49638944753667	34.28852939923102	19.334944896632216
14	19.21569287828321	25.28115295738132	36.21508527722142	19.288068887114047
15	24.46694004132237	22.145649672624	33.55274100936008	19.83466927669355
16	23.85174396626027	22.821330921257925	30.332867780785232	22.994057331696567
17	24.65248518456913	24.333082228218807	30.678973566604483	20.335459020607573
18	25.178156329429747	28.340774842566717	27.540927155231078	18.940141672772462
19	24.138223742905023	26.517696724590383	27.71715895641081	21.626920576093784
20	24.973984502048935	25.53226952743209	30.110412935029302	19.383333035489674
21	21.618260198968727	22.32160654119659	30.715711436234194	25.344421823600488
22	20.905222482339017	27.42439009637763	30.02212520155997	21.648262219723392
23	20.386287186353442	28.955214852959166	29.867647442920553	20.79085051776683
24	25.67248515707587	25.39311926164099	28.089383339908803	20.845012241374334
25	20.64183704469442	25.77627221627299	28.609762032081886	24.97212870695071
26	19.309994762533833	24.18994543962411	32.64941556201036	23.85064423583169
27	20.371818857902458	25.031445416942173	31.508513975511754	23.08822174964362
28	22.047120699538524	27.542473651146267	28.155160966168168	22.255244683147044
29	23.86232887163534	23.98979450162279	30.35365955920055	21.794217067541318
30	24.994501347857106	25.415354436243813	27.61172230157083	21.97842191432825
31	25.766099709808632	26.390231094602935	26.056737842136446	21.786931353451987
32	24.189326841258037	25.857102402773517	25.50123650940063	24.452334246567812
33	19.794047983987927	27.638906263102257	28.838299761770898	23.728745991138922
34	20.163591774566257	30.783963455957856	26.184237838699786	22.868206930776093
35	25.96013339730099	29.759908227495735	24.08540231575735	20.19455605944593
36	21.877934046416872	30.480197291638888	24.554162410939018	23.087706251005223
37	23.335111230859535	29.387133978783453	26.188086895199813	21.0896678951572
38	22.30456071955362	26.811187282717324	25.727128012745876	25.157123984983183
39	24.68100944256039	25.39995821024371	29.217741126206437	20.701291220989454
40	20.800266959561537	24.908447441820826	30.263894062967818	24.027391535649826
41	21.3386193709267	27.867340893063584	28.323454088316602	22.47058564769311
42	21.250537836912727	24.810743266556784	29.017590188205116	24.921128708325373
43	23.21671837690786	25.976938652912708	29.984184501774003	20.822158468405433
44	22.722252082958164	26.306342282847915	27.353938615796803	23.617467018397114
45	23.18874398413089	26.079797814560706	27.768227688187935	22.96323051312047
46	23.058700860951458	26.665438634354754	29.17089948326416	21.10496102142962
47	22.653725130627357	28.91541835807498	26.982023531481847	21.448832979815823
48	22.56017931104638	27.443532279150073	27.075363151607462	22.920925258196085
49	20.830200247164413	26.666710197662795	28.961057170860993	23.542032384311796
50	21.391131498891333	26.56457273410855	29.59171820507498	22.452577561925132
51	21.003407789665555	25.73998111212989	29.027797061245366	24.22881403695919
52	20.84721170223149	24.88659029955282	28.30475867103076	25.961439327184927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2067.0
1	3597.5
2	5128.0
3	28521.5
4	51915.0
5	31981.0
6	12047.0
7	12403.5
8	12760.0
9	14358.0
10	15956.0
11	16876.5
12	17797.0
13	17907.0
14	16666.0
15	15315.0
16	14018.0
17	12721.0
18	12298.0
19	11875.0
20	11460.5
21	11046.0
22	11737.0
23	12428.0
24	13921.0
25	15414.0
26	17665.0
27	19916.0
28	22805.5
29	25695.0
30	29352.5
31	33010.0
32	38478.0
33	43946.0
34	51341.0
35	58736.0
36	65719.5
37	72703.0
38	78512.0
39	87577.5
40	90834.0
41	106523.5
42	122213.0
43	139303.5
44	156394.0
45	177298.5
46	198203.0
47	209325.5
48	220448.0
49	240786.0
50	261124.0
51	243562.5
52	226001.0
53	211273.0
54	196545.0
55	183626.5
56	170708.0
57	163385.5
58	156063.0
59	148176.5
60	140290.0
61	125545.5
62	110801.0
63	99818.0
64	78092.5
65	67350.0
66	58830.5
67	50311.0
68	42729.0
69	35147.0
70	29310.0
71	23473.0
72	20523.0
73	17573.0
74	14128.0
75	10683.0
76	9527.5
77	8372.0
78	5633.0
79	2894.0
80	2133.5
81	1373.0
82	1114.5
83	856.0
84	554.0
85	252.0
86	203.0
87	154.0
88	102.5
89	30.5
90	10.0
91	18.5
92	27.0
93	15.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2909804.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.417574196000118
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41995444150452	15.194749414723674
2	8.6357392001317	3.5264169171199145
3	3.2419832032651095	1.9858029778455448
4	1.7762087651781129	1.450634970024395
5	1.148953748618465	1.172942420509499
6	0.8443411698668157	1.034363908949595
7	0.6755649455661022	0.9655378180218872
8	0.5399624179818107	0.8819778185756199
9	0.43757730615058	0.8040840403333794
>10	5.987817043537583	29.63043202081223
>50	1.8365150349218973	26.426807260384617
>100	0.44451284870844615	11.910486199117226
>500	0.006623829815446233	0.9406291816642826
>1k	0.0033968358027929397	1.3125114800667808
>5k	1.69841790139647E-4	0.1753298117048705
>10k+	6.79367160558588E-4	2.5872937601464376
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37932	1.3035929567764701	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	15480	0.5319945948249435	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	11030	0.3790633321007188	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10168	0.34943934368088025	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5056	0.17375740771543377	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4094	0.14069676170628675	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2953	0.10148449861227768	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0581138798352054	0.0
2	0.0	0.0	0.0	0.16107614121088568	0.0
3	0.0	0.0	0.0	0.3207432528101549	0.0
4	0.0	0.0	0.0	0.4620242463066241	0.0
5	0.0	0.0	0.0	1.255411017374366	0.0
6	0.0	0.0	0.0	1.7085343205246815	0.0
7	0.0	0.0	0.0	2.657670413539881	0.0
8	0.0	0.0	0.0	3.7189102771183213	0.0
9	0.0	0.0	0.0	5.123712799899924	0.0
10	0.0	0.0	0.0	5.861838116931587	0.0
11	3.4366575893084206E-5	0.0	0.0	6.329292282229319	0.0
12	3.4366575893084206E-5	0.0	0.0	6.660379874383292	0.0
13	3.4366575893084206E-5	0.0	0.0	6.933903451916349	0.0
14	3.4366575893084206E-5	0.0	0.0	7.0452511578099415	0.0
15	6.873315178616841E-5	0.0	0.0	7.237016651293351	0.0
16	1.0309972767925263E-4	0.0	0.0	7.485177695817312	0.0
17	1.3746630357233683E-4	0.0	0.0	7.805474183140857	0.0
18	1.7183287946542105E-4	0.0	0.0	7.971808410463385	0.0
19	1.7183287946542105E-4	0.0	0.0	8.154535494486915	0.0
20	1.7183287946542105E-4	0.0	0.0	8.38214532662681	0.0
21	2.0619945535850525E-4	0.0	0.0	8.635701923565986	0.0
22	2.0619945535850525E-4	0.0	0.0	8.881354208049752	0.0
23	2.0619945535850525E-4	0.0	0.0	9.074838030327816	0.0
24	2.0619945535850525E-4	0.0	0.0	9.236429670177097	0.0
25	2.0619945535850525E-4	0.0	0.0	9.408193816490733	0.0
26	2.0619945535850525E-4	0.0	0.0	9.62322548185376	0.0
27	2.4056603125158945E-4	0.0	0.0	9.828084640752435	0.0
28	2.4056603125158945E-4	0.0	0.0	10.065454580445968	0.0
29	2.4056603125158945E-4	0.0	0.0	10.27763382000987	0.0
30	2.4056603125158945E-4	0.0	0.0	10.51074230429266	0.0
31	2.4056603125158945E-4	0.0	0.0	10.70975914528951	0.0
32	2.4056603125158945E-4	0.0	0.0	10.957026658840253	0.0
33	2.4056603125158945E-4	0.0	0.0	11.218934333721446	0.0
34	2.7493260714467365E-4	0.0	0.0	11.543182977272696	0.0
35	2.7493260714467365E-4	0.0	0.0	11.77220183902421	0.0
36	2.7493260714467365E-4	0.0	0.0	12.027889163668755	0.0
37	2.7493260714467365E-4	0.0	0.0	12.401866242537299	0.0
38	2.7493260714467365E-4	0.0	0.0	13.25910611161439	0.0
39	2.7493260714467365E-4	0.0	0.0	13.637344645893675	0.0
40	2.7493260714467365E-4	0.0	0.0	14.022868894262293	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAACG	20	6.313056E-4	46.0	16
CGTTTTT	14410	0.0	43.254684	1
CCCGCAC	6755	0.0	41.743893	32
CCGCACT	6670	0.0	41.655174	33
CGCACTT	6675	0.0	41.58951	34
ATAGCGG	445	0.0	41.348316	1
CTAGCGG	690	0.0	41.333332	1
TCACGAC	430	0.0	41.186047	24
CGGTCTA	425	0.0	41.129414	30
ACCCGCA	6835	0.0	41.053402	31
TACGCGG	370	0.0	41.027027	1
CACTTAC	6800	0.0	40.994118	36
ATCAACG	7365	0.0	40.59742	14
TATGCGG	510	0.0	40.58823	1
CGCAAGC	7325	0.0	40.473717	19
AACGCAA	7335	0.0	40.449894	17
GCACTTA	6875	0.0	40.41309	35
TCAACGC	7365	0.0	40.410046	15
ACGCAAG	7370	0.0	40.35142	18
CAACGCA	7375	0.0	40.32407	16
>>END_MODULE