FastQCFastQC Report
Sat 14 Jan 2017
SRR2933101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368302
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC83260.6084914002902868No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.40568529462063196No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC44260.3234666031329341No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC42800.31279644405986395No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT23500.1717457111076356TruSeq Adapter, Index 15 (95% over 23bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23280.17013787891854282No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC20440.14938222702298176No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT17990.13147682309899422TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17960.13125757325502702No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT16490.12051433090063451TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG351.01976184E-746.00000420
TCGCATA206.3118787E-446.039
TTTACGG800.046.01
CGTGCTA206.3118787E-446.032
GAATCGC206.3118787E-446.024
CCGTGCT206.3118787E-446.031
TAACGTT206.3118787E-446.023
CGCGACA206.3118787E-446.03
CGTTAGT206.3118787E-446.044
CGTAGCA301.8616647E-646.032
CGAGGTA301.8616647E-646.044
TATTCGA253.4175006E-546.039
CCGAGTA206.3118787E-446.020
CGTACAG301.8616647E-646.029
ACGTCGG206.3118787E-446.01
CGTAAGA206.3118787E-446.028
GTATACG206.3118787E-446.015
TATAGCG253.4175006E-546.019
ACCGTAG206.3118787E-446.038
CGCACGA253.4175006E-546.038
Produced by FastQC (version 0.11.5)