##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368302
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.558230566059244	33.0	31.0	34.0	31.0	34.0
2	32.92945197770668	34.0	31.0	34.0	31.0	34.0
3	33.01197542647749	34.0	33.0	34.0	31.0	34.0
4	36.40629846335093	37.0	37.0	37.0	35.0	37.0
5	36.38414692078211	37.0	37.0	37.0	35.0	37.0
6	36.52425487940528	37.0	37.0	37.0	35.0	37.0
7	36.576924538588706	37.0	37.0	37.0	35.0	37.0
8	36.4397713370294	37.0	37.0	37.0	35.0	37.0
9	38.082426978839464	39.0	39.0	39.0	35.0	39.0
10	37.93537610849067	39.0	38.0	39.0	35.0	39.0
11	37.385028305154854	39.0	35.0	39.0	35.0	39.0
12	37.2045162544526	39.0	35.0	39.0	35.0	39.0
13	37.19922283238642	39.0	35.0	39.0	35.0	39.0
14	38.56750044946218	40.0	36.0	41.0	35.0	41.0
15	38.64663283397963	41.0	36.0	41.0	35.0	41.0
16	38.63429929942367	40.0	36.0	41.0	35.0	41.0
17	38.648000222173174	40.0	36.0	41.0	35.0	41.0
18	38.594184617138616	40.0	36.0	41.0	35.0	41.0
19	38.483043217067575	40.0	36.0	41.0	35.0	41.0
20	38.387621299976175	40.0	35.0	41.0	35.0	41.0
21	38.33669321538666	40.0	35.0	41.0	35.0	41.0
22	38.32250555798355	40.0	35.0	41.0	35.0	41.0
23	38.29454316371678	40.0	35.0	41.0	35.0	41.0
24	38.2379577023201	40.0	35.0	41.0	35.0	41.0
25	38.186885643666386	40.0	35.0	41.0	35.0	41.0
26	38.117094033334745	40.0	35.0	41.0	35.0	41.0
27	38.093043787117175	40.0	35.0	41.0	35.0	41.0
28	38.09734619988862	40.0	35.0	41.0	35.0	41.0
29	38.08692160064079	40.0	36.0	41.0	35.0	41.0
30	38.00287144212316	40.0	35.0	41.0	35.0	41.0
31	37.92224450450266	40.0	35.0	41.0	34.0	41.0
32	37.843220283241564	40.0	35.0	41.0	34.0	41.0
33	37.709470570093444	40.0	35.0	41.0	34.0	41.0
34	37.60150975442556	40.0	35.0	41.0	34.0	41.0
35	37.483086336203556	40.0	35.0	41.0	34.0	41.0
36	37.429673420049085	40.0	35.0	41.0	34.0	41.0
37	37.31670201461373	40.0	35.0	41.0	33.0	41.0
38	37.34180246758391	40.0	35.0	41.0	34.0	41.0
39	37.225127201451144	40.0	35.0	41.0	33.0	41.0
40	37.22235149842652	39.0	35.0	41.0	33.0	41.0
41	37.17566151332089	39.0	35.0	41.0	33.0	41.0
42	37.12979810012702	39.0	35.0	41.0	33.0	41.0
43	37.04052248699483	39.0	35.0	41.0	33.0	41.0
44	36.95920710486428	39.0	35.0	41.0	33.0	41.0
45	36.89132954567047	39.0	35.0	41.0	33.0	41.0
46	36.82247705550383	39.0	35.0	41.0	33.0	41.0
47	36.75146495437411	38.0	35.0	41.0	33.0	41.0
48	36.71047838854288	38.0	35.0	41.0	33.0	41.0
49	36.6176165787962	38.0	35.0	41.0	33.0	41.0
50	36.51999631660262	38.0	35.0	41.0	33.0	41.0
51	36.39546752105895	38.0	35.0	41.0	32.0	41.0
52	36.16204536717771	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	10.0
13	7.0
14	7.0
15	14.0
16	39.0
17	90.0
18	165.0
19	314.0
20	575.0
21	927.0
22	1571.0
23	2370.0
24	4014.0
25	6329.0
26	8558.0
27	10186.0
28	10715.0
29	11699.0
30	14221.0
31	17338.0
32	22859.0
33	31526.0
34	81254.0
35	208336.0
36	69126.0
37	98773.0
38	163455.0
39	596814.0
40	7008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.7515416918195	22.47179350757362	25.530986580447884	19.245678220159
2	27.694251707590865	24.369035490703077	27.349883285999727	20.586829515706327
3	28.139694307250885	24.874917964016717	25.318021898674413	21.66736583005798
4	24.671015609127224	27.468132035179366	24.102354597157646	23.758497758535764
5	21.743007026226664	38.03582834783549	21.591505384045334	18.629659241892508
6	88.95426594421407	3.092445965875954	5.87713823410329	2.076149855806686
7	91.22247866333602	2.1042869191158093	4.4316240128275775	2.2416104047205954
8	84.85648636046722	3.9479588570359465	7.5546918735776165	3.64086290891923
9	46.42556979380283	25.016261030094235	13.535389117314745	15.022780058788191
10	37.32450877072459	22.723930828135895	22.17982579869064	17.771734602448873
11	34.12411879833546	20.20833120173763	27.65105948832933	18.016490511597585
12	21.97387711192412	32.57950364758657	27.052580497580216	18.394038742909093
13	17.466319569802575	36.341246303813044	28.44372075755206	17.74871336883232
14	16.50900166776048	26.053751291746995	38.86481200787546	18.57243503261707
15	18.112083443567283	20.7701223852629	39.922619421735845	21.19517474943397
16	18.284998487176075	21.72290912386301	28.08751284438669	31.904579544574226
17	21.31466591439609	24.401630634172868	31.71909417657798	22.56460927485307
18	25.412006998455016	28.344108245109634	26.07648019223826	20.167404564197085
19	27.089926054335955	23.393519851611703	25.7271421075172	23.789411986535136
20	22.935799260689528	27.6878203788345	28.63066779117476	20.745712569301222
21	20.930540187765565	24.117921336079316	26.540339778791523	28.411198697363595
22	17.907888755552502	29.690886953318785	25.151611267103313	27.2496130240254
23	18.650487977069393	26.423918111644944	34.58965930035913	20.335934610926536
24	21.43291466357573	24.127860662339163	31.617873831946458	22.821350842138653
25	17.413991940375737	31.12178451833002	26.856936553480153	24.607286987814096
26	17.088479005365777	29.864094330052872	29.326128296238696	23.721298368342662
27	18.90854504341878	26.690525921909053	34.37508678639657	20.025842248275598
28	18.00421252033542	26.462944583871106	30.970575209273974	24.562267686519494
29	20.160023152783523	28.466668907887293	30.160008536127258	21.213299403201923
30	27.018669855046618	26.52396912377531	25.615543936937897	20.841817084240173
31	26.644410371394617	27.686870296177307	24.938573502048524	20.730145830379552
32	25.17185533602962	27.0368675920959	23.912776565407345	23.87850050646714
33	19.977241866196206	26.296607035581328	27.808261626453813	25.917889471768657
34	21.17039951706568	25.33680430197427	28.26152413721532	25.231272043744728
35	27.388178925412664	24.859278141813725	26.99499087189816	20.75755206087545
36	22.778889455690337	28.759440532864822	26.9441979913791	21.517472020065746
37	22.1676208907098	28.874839034072885	27.4033071646464	21.55423291057091
38	21.116098639043134	26.877180622406456	26.695057085351042	25.311663653199368
39	23.777133995272973	23.87908517271772	30.155404289403947	22.188376542605358
40	19.451334573800228	23.560807482558673	31.042123741688606	25.94573420195249
41	22.001721842107955	27.647039907856595	24.909413272800887	25.441824977234557
42	21.567460984490268	23.587409796960028	28.17652828103737	26.66860093751233
43	21.665173331618313	25.377438606389525	30.403814362618782	22.553573699373384
44	20.84561741486894	30.28929286078658	25.43670914754199	23.42838057680249
45	23.549406490672382	27.618829761266156	27.309175898303153	21.522587849758313
46	24.403165383080637	26.197798439233445	26.39885054615136	23.00018563153456
47	21.673212492563778	25.593692035822503	30.520674529453295	22.21242094216043
48	22.012099668055736	23.946541041378293	29.137500347145583	24.903858943420385
49	21.07202942040573	26.6772978479897	29.870964158497177	22.379708573107397
50	20.210304450333332	27.09635738309233	29.394972747244395	23.298365419329944
51	20.965620162800317	22.831144001835852	30.72574621684394	25.47748961851989
52	23.14284419667588	23.299023168861847	29.45512028777273	24.10301234668955
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1947.0
1	1852.5
2	1758.0
3	4906.5
4	8055.0
5	5232.0
6	2409.0
7	2622.0
8	2835.0
9	3132.0
10	3429.0
11	3523.5
12	3618.0
13	3741.5
14	3833.5
15	3802.0
16	3590.5
17	3379.0
18	3350.0
19	3321.0
20	3453.5
21	3586.0
22	3907.0
23	4228.0
24	4335.0
25	4442.0
26	5952.0
27	7462.0
28	8702.0
29	9942.0
30	11902.0
31	13862.0
32	15336.0
33	16810.0
34	20902.5
35	24995.0
36	31877.0
37	38759.0
38	39848.0
39	44451.5
40	47966.0
41	58776.5
42	69587.0
43	87818.0
44	106049.0
45	116156.0
46	126263.0
47	132739.0
48	139215.0
49	143003.0
50	146791.0
51	130979.0
52	115167.0
53	101545.5
54	87924.0
55	81046.0
56	74168.0
57	67707.5
58	61247.0
59	56165.5
60	51084.0
61	46791.5
62	42499.0
63	36977.0
64	26856.0
65	22257.0
66	18057.5
67	13858.0
68	11684.0
69	9510.0
70	8129.0
71	6748.0
72	6655.0
73	6562.0
74	4879.5
75	3197.0
76	2282.0
77	1367.0
78	1054.5
79	742.0
80	689.0
81	636.0
82	445.0
83	254.0
84	237.5
85	221.0
86	148.0
87	75.0
88	42.0
89	5.5
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1368302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.48336223047856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.39661305573699	13.910460741942382
2	11.95934834205028	4.660166315772775
3	4.599250847885672	2.6882661077457657
4	2.36393514615958	1.842296189679455
5	1.3306340398094494	1.2962612496906263
6	0.826898914020644	0.966646264191213
7	0.5840689059292801	0.7965738243245628
8	0.4761245640015973	0.7421205877817434
9	0.3455160409017696	0.605863276319702
>10	3.5676457900211322	15.903611840346791
>50	1.3364680145979797	19.00978631724795
>100	1.202557142793725	34.06552665186837
>500	0.006789845850152757	0.8975075487796851
>1k	0.0033949229250763787	1.5950381863057244
>5k	7.544273166836398E-4	1.01987489800325
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	8326	0.6084914002902868	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5551	0.40568529462063196	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4426	0.3234666031329341	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4280	0.31279644405986395	No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT	2350	0.1717457111076356	TruSeq Adapter, Index 15 (95% over 23bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2328	0.17013787891854282	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2044	0.14938222702298176	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1799	0.13147682309899422	TruSeq Adapter, Index 14 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1796	0.13125757325502702	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1649	0.12051433090063451	TruSeq Adapter, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17788470673871704	0.0
2	0.0	0.0	0.0	0.5325578709963151	0.0
3	0.0	0.0	0.0	0.9349544179574392	0.0
4	0.0	0.0	0.0	1.2060933916635364	0.0
5	0.0	0.0	0.0	2.6603776066979368	0.0
6	0.0	0.0	0.0	2.9253045014916297	0.0
7	0.0	0.0	0.0	3.7208891019672556	0.0
8	0.0	0.0	0.0	4.728122885152547	0.0
9	7.308328132239812E-5	0.0	0.0	5.650433895441211	0.0
10	7.308328132239812E-5	0.0	0.0	6.3454559008172176	0.0
11	7.308328132239812E-5	0.0	0.0	7.080673710920542	0.0
12	7.308328132239812E-5	0.0	0.0	7.631210069122168	0.0
13	1.4616656264479624E-4	0.0	0.0	7.852506244966389	0.0
14	1.4616656264479624E-4	0.0	0.0	8.000719139488213	0.0
15	1.4616656264479624E-4	0.0	0.0	8.518514187657404	0.0
16	1.4616656264479624E-4	0.0	0.0	9.182548881752712	0.0
17	1.4616656264479624E-4	0.0	0.0	10.05282459573983	0.0
18	1.4616656264479624E-4	0.0	0.0	10.443820150814659	0.0
19	1.4616656264479624E-4	0.0	0.0	10.904756406115025	0.0
20	1.4616656264479624E-4	0.0	0.0	11.416047042246522	0.0
21	2.192498439671944E-4	0.0	0.0	11.915644353366435	0.0
22	2.192498439671944E-4	0.0	0.0	12.48291678299089	0.0
23	2.192498439671944E-4	0.0	0.0	12.909650062632371	0.0
24	3.6541640661199063E-4	0.0	0.0	13.24970657062549	0.0
25	3.6541640661199063E-4	0.0	0.0	13.579969918921408	0.0
26	3.6541640661199063E-4	0.0	0.0	13.915349096909893	0.0
27	3.6541640661199063E-4	0.0	0.0	14.300571072760253	0.0
28	5.115829692567869E-4	0.0	0.0	14.694709208931947	0.0
29	5.115829692567869E-4	0.0	0.0	15.106094999495726	0.0
30	5.115829692567869E-4	0.0	0.0	15.491901641596666	0.0
31	5.84666250579185E-4	0.0	0.0	15.817999242857205	0.0
32	5.84666250579185E-4	0.0	0.0	16.186923646972673	0.0
33	5.84666250579185E-4	0.0	0.0	16.53925814622795	0.0
34	5.84666250579185E-4	0.0	0.0	17.007941229348493	0.0
35	5.84666250579185E-4	0.0	0.0	17.353990566410047	0.0
36	5.84666250579185E-4	0.0	0.0	17.72371888662006	0.0
37	5.84666250579185E-4	0.0	0.0	18.101778700900823	0.0
38	5.84666250579185E-4	0.0	0.0	18.517476405062624	0.0
39	5.84666250579185E-4	0.0	0.0	18.937705272666413	0.0
40	5.84666250579185E-4	0.0	0.0	19.325192830237768	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	35	1.01976184E-7	46.000004	20
TCGCATA	20	6.3118787E-4	46.0	39
TTTACGG	80	0.0	46.0	1
CGTGCTA	20	6.3118787E-4	46.0	32
GAATCGC	20	6.3118787E-4	46.0	24
CCGTGCT	20	6.3118787E-4	46.0	31
TAACGTT	20	6.3118787E-4	46.0	23
CGCGACA	20	6.3118787E-4	46.0	3
CGTTAGT	20	6.3118787E-4	46.0	44
CGTAGCA	30	1.8616647E-6	46.0	32
CGAGGTA	30	1.8616647E-6	46.0	44
TATTCGA	25	3.4175006E-5	46.0	39
CCGAGTA	20	6.3118787E-4	46.0	20
CGTACAG	30	1.8616647E-6	46.0	29
ACGTCGG	20	6.3118787E-4	46.0	1
CGTAAGA	20	6.3118787E-4	46.0	28
GTATACG	20	6.3118787E-4	46.0	15
TATAGCG	25	3.4175006E-5	46.0	19
ACCGTAG	20	6.3118787E-4	46.0	38
CGCACGA	25	3.4175006E-5	46.0	38
>>END_MODULE