##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933100.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1484810
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.511050572127075	33.0	31.0	34.0	31.0	34.0
2	32.86866804506974	34.0	31.0	34.0	31.0	34.0
3	32.962127140846306	34.0	31.0	34.0	31.0	34.0
4	36.38457917174588	37.0	37.0	37.0	35.0	37.0
5	36.360773432291	37.0	37.0	37.0	35.0	37.0
6	36.49115105636412	37.0	37.0	37.0	35.0	37.0
7	36.525678032879625	37.0	37.0	37.0	35.0	37.0
8	36.37507425192449	37.0	37.0	37.0	35.0	37.0
9	38.015296906674926	39.0	38.0	39.0	35.0	39.0
10	37.918015773061875	39.0	38.0	39.0	35.0	39.0
11	37.48726099635643	39.0	37.0	39.0	35.0	39.0
12	37.307686505344115	39.0	37.0	39.0	35.0	39.0
13	37.07319791757868	39.0	37.0	39.0	33.0	39.0
14	38.58617466207798	41.0	38.0	41.0	34.0	41.0
15	38.71671729042773	41.0	38.0	41.0	35.0	41.0
16	38.736028852176375	41.0	37.0	41.0	35.0	41.0
17	38.74729022568544	41.0	37.0	41.0	35.0	41.0
18	38.6728833992228	40.0	37.0	41.0	35.0	41.0
19	38.54598029377496	40.0	37.0	41.0	35.0	41.0
20	38.458329348536175	40.0	36.0	41.0	35.0	41.0
21	38.45729352577097	40.0	36.0	41.0	35.0	41.0
22	38.40848054633253	40.0	35.0	41.0	35.0	41.0
23	38.361114890120625	40.0	35.0	41.0	35.0	41.0
24	38.2961995137425	40.0	35.0	41.0	35.0	41.0
25	38.220502959974674	40.0	35.0	41.0	34.0	41.0
26	38.12996208269072	40.0	35.0	41.0	34.0	41.0
27	38.062587805847215	40.0	35.0	41.0	34.0	41.0
28	38.041949475017006	40.0	35.0	41.0	34.0	41.0
29	38.022370538991524	40.0	35.0	41.0	34.0	41.0
30	37.919048901879705	40.0	35.0	41.0	34.0	41.0
31	37.85458004727877	40.0	35.0	41.0	34.0	41.0
32	37.783042948256	40.0	35.0	41.0	34.0	41.0
33	37.612303931142705	40.0	35.0	41.0	34.0	41.0
34	37.504042941521135	40.0	35.0	41.0	33.0	41.0
35	37.38696870306639	40.0	35.0	41.0	33.0	41.0
36	37.29079545531078	40.0	35.0	41.0	33.0	41.0
37	37.18494352812818	40.0	35.0	41.0	33.0	41.0
38	37.182990416282216	40.0	35.0	41.0	33.0	41.0
39	37.07048578606017	40.0	35.0	41.0	33.0	41.0
40	36.9842181827978	39.0	35.0	41.0	33.0	41.0
41	36.97412530896209	39.0	35.0	41.0	33.0	41.0
42	36.930577649665615	39.0	35.0	41.0	33.0	41.0
43	36.85806534169355	39.0	35.0	41.0	33.0	41.0
44	36.77157750823338	39.0	35.0	41.0	33.0	41.0
45	36.72815511748978	39.0	35.0	41.0	33.0	41.0
46	36.62607808406463	39.0	35.0	41.0	32.0	41.0
47	36.5290299768994	38.0	35.0	41.0	32.0	41.0
48	36.46389504381032	38.0	35.0	41.0	32.0	41.0
49	36.36741468605411	38.0	35.0	41.0	32.0	41.0
50	36.25884793340562	38.0	35.0	41.0	32.0	41.0
51	36.17050060277072	38.0	35.0	41.0	31.0	41.0
52	35.90260235316303	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	4.0
13	6.0
14	14.0
15	22.0
16	60.0
17	119.0
18	258.0
19	482.0
20	962.0
21	1473.0
22	2275.0
23	3314.0
24	5120.0
25	8237.0
26	11316.0
27	13226.0
28	13925.0
29	15058.0
30	17352.0
31	21146.0
32	27059.0
33	37988.0
34	97899.0
35	161695.0
36	87001.0
37	117992.0
38	191861.0
39	640465.0
40	8475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.5218445457668	21.63313824664435	23.16929438783413	18.675722819754718
2	28.76980893178252	24.230103514927837	26.36869363756979	20.631393915719855
3	30.00787979606818	24.123557896296496	24.8830490096376	20.985513297997723
4	23.49822536216755	26.69647968426937	23.35261750661701	26.452677446946073
5	22.100740162040935	39.35096072898216	21.57616125968979	16.972137849287115
6	87.75991541005247	3.108949966662401	6.887345855698709	2.2437887675864254
7	89.30334520915135	2.4679925377657748	5.631696984799402	2.596965268283484
8	82.50449552467992	4.5807881143041875	8.752432971221907	4.162283389793981
9	40.313642822987454	30.498717007563258	14.899280042564369	14.288360126884921
10	34.29751954795563	22.379563715222822	23.911274843245938	19.41164189357561
11	32.292751261104115	20.227167112290463	29.41029491988874	18.069786706716684
12	24.79293646998606	25.45813942524633	31.54295835830847	18.205965746459142
13	18.58628376694661	31.69685010203326	31.22837265374021	18.48849347727992
14	16.755207737016857	28.070729588297493	37.213515533974046	17.960547140711604
15	21.83100868124541	20.917289080757804	37.861611923411076	19.390090314585702
16	22.44758588640971	22.010762319758083	27.78712427852722	27.75452751530499
17	24.241081350475817	26.699443026380482	28.550791010297615	20.508684612846086
18	25.74491012318074	29.9125814077222	25.117893871943213	19.224614597153845
19	24.222829857018745	26.643139526269355	25.30539260915538	23.82863800755652
20	24.688276614516337	25.947023524895442	29.389955617217016	19.974744243371205
21	20.29094631636371	23.255298657740724	29.614630828186773	26.8391241977088
22	18.847259918777485	30.179416895090956	27.76429307453479	23.20903011159677
23	19.45770839366653	30.803739198954748	29.49030515688876	20.24824725048996
24	26.11795448575912	24.748351640950695	27.363366356638224	21.770327516651964
25	19.194644432621008	27.363433705322564	25.72255035997872	27.719371502077706
26	17.18004323785535	26.990658737481567	32.59629178144005	23.233006243223038
27	19.432115893616018	25.620853846620108	32.36858587967484	22.578444380089035
28	19.36793259743671	30.489018797017803	27.747994692923672	22.39505391262182
29	22.500252557566288	24.969524720334586	30.330547342757658	22.199675379341464
30	26.960419178211353	25.540708912251397	26.009119011860104	21.489752897677146
31	25.71500730733225	27.961759417029786	25.302227220991234	21.021006054646723
32	24.388911712609694	26.492682565446085	23.075881762649768	26.042523959294456
33	18.95656683346691	25.49316074110492	27.864373219469158	27.685899205959014
34	20.518719566813264	26.910513803112856	26.297438729534417	26.27332790053946
35	27.970514745994436	26.40775587448899	23.64652716509183	21.97520221442474
36	21.56673244388171	30.594958277490047	23.635145237437786	24.203164041190455
37	23.10827647981897	30.056168802742437	24.26317171894047	22.572382998498124
38	20.5085499154774	26.137754998956098	24.1415399950162	29.212155090550308
39	24.461715640384966	24.98083929930429	28.338103865140994	22.219341195169754
40	19.49400933452765	23.861840908937847	30.727769883015338	25.91637987351917
41	20.218613829378842	29.065267609997235	25.819667162801974	24.896451397821945
42	20.199756197762678	24.63372418019814	27.770623850863075	27.39589577117611
43	21.921457964318666	26.75998949360524	29.803409190401464	21.515143351674627
44	22.033189431644455	27.5873007320802	24.793946700251208	25.585563136024135
45	23.348643934240744	25.341289457910438	26.525818118143064	24.784248489705753
46	24.208619284622273	26.118425926549527	27.270761915665975	22.40219287316222
47	20.46679373118446	30.350280507270288	26.645362032852688	22.53756372869256
48	21.118459600891697	27.540762791198876	26.75035863174413	24.590418976165303
49	19.443161077848345	27.078144678443707	29.6569931506388	23.821701093069148
50	19.29048161044174	27.87009785763835	29.216263360295258	23.623157171624655
51	20.40894121133344	25.517002175362503	26.807605013436064	27.266451599867995
52	21.110445107454826	24.167671284541456	27.02749846781743	27.694385140186284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1550.0
1	1919.5
2	2289.0
3	8161.5
4	14034.0
5	8777.5
6	3521.0
7	3903.5
8	4286.0
9	5177.0
10	6068.0
11	6736.0
12	7404.0
13	7555.0
14	7448.5
15	7191.0
16	6708.5
17	6226.0
18	5993.5
19	5761.0
20	5512.0
21	5263.0
22	5124.5
23	4986.0
24	5573.0
25	6160.0
26	6498.5
27	6837.0
28	9791.5
29	12746.0
30	14500.0
31	16254.0
32	18168.0
33	20082.0
34	23491.5
35	26901.0
36	30585.0
37	34269.0
38	38283.5
39	45372.5
40	48447.0
41	55752.5
42	63058.0
43	73860.0
44	84662.0
45	88371.5
46	92081.0
47	100089.5
48	108098.0
49	131905.5
50	155713.0
51	150964.5
52	146216.0
53	129930.0
54	113644.0
55	104048.0
56	94452.0
57	88228.0
58	82004.0
59	76288.0
60	70572.0
61	64287.0
62	58002.0
63	51306.5
64	36620.5
65	28630.0
66	22852.0
67	17074.0
68	14799.0
69	12524.0
70	10481.0
71	8438.0
72	7460.0
73	6482.0
74	4921.5
75	3361.0
76	3093.0
77	2825.0
78	1985.5
79	1146.0
80	823.0
81	500.0
82	363.0
83	226.0
84	195.5
85	165.0
86	94.5
87	24.0
88	18.0
89	9.0
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1484810.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.367958568827465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78267605350328	15.765851650084766
2	10.18198664820159	4.351365376942521
3	3.7395903931664574	2.3972223775669823
4	1.8568581615786814	1.5870907305920963
5	1.1324760162212364	1.209935029740308
6	0.8048802914380497	1.0319189232188415
7	0.5889028212958853	0.8808555760561257
8	0.4679786607305373	0.7999798906868388
9	0.40854331585160086	0.7856762982019561
>10	4.810747235446005	24.33388945176774
>50	1.6075392490325204	24.713748252000713
>100	0.6076929379025504	17.028816728499965
>500	0.005380614554297782	0.7617959078139277
>1k	0.003481574123369153	1.363509170582084
>5k	3.1650673848810485E-4	0.6583880648587168
>10k+	9.495202154643145E-4	2.3299565713865076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13821	0.9308261663108411	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10375	0.6987426000633078	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10255	0.6906607579420936	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9735	0.6556394420834989	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4290	0.2889258558334063	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3475	0.23403667809349343	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	1779	0.11981330944699996	Illumina Single End Adapter 2 (95% over 21bp)
GGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1656	0.11152942127275543	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1244603686666981	0.0
2	0.0	0.0	0.0	0.3613256915026165	0.0
3	0.0	0.0	0.0	0.6715337315885535	0.0
4	0.0	0.0	0.0	0.9145277846997258	0.0
5	0.0	0.0	0.0	2.2216310504374297	0.0
6	0.0	0.0	0.0	2.5696217024400427	0.0
7	0.0	0.0	0.0	3.4192253554326815	0.0
8	0.0	0.0	0.0	4.539974811592056	0.0
9	0.0	0.0	0.0	5.462382392359965	0.0
10	0.0	0.0	0.0	6.154726867410645	0.0
11	0.0	0.0	0.0	6.806258039749194	0.0
12	0.0	0.0	0.0	7.28389490911295	0.0
13	0.0	0.0	0.0	7.497188192428661	0.0
14	0.0	0.0	0.0	7.615991271610509	0.0
15	0.0	0.0	0.0	7.9694371670449415	0.0
16	0.0	0.0	0.0	8.43636559559809	0.0
17	0.0	0.0	0.0	9.039473063893697	0.0
18	0.0	0.0	0.0	9.331362261838215	0.0
19	0.0	0.0	0.0	9.641031512449404	0.0
20	6.734868434345135E-5	0.0	0.0	10.045393013247486	0.0
21	6.734868434345135E-5	0.0	0.0	10.469083586452138	0.0
22	1.346973686869027E-4	0.0	0.0	10.86718165960628	0.0
23	1.346973686869027E-4	0.0	0.0	11.150248179901805	0.0
24	1.346973686869027E-4	0.0	0.0	11.376607107980146	0.0
25	2.0204605303035406E-4	0.0	0.0	11.612462200550912	0.0
26	2.693947373738054E-4	0.0	0.0	11.906843299816138	0.0
27	2.693947373738054E-4	0.0	0.0	12.20290811618995	0.0
28	2.693947373738054E-4	0.0	0.0	12.52800021551579	0.0
29	2.693947373738054E-4	0.0	0.0	12.805544143695153	0.0
30	2.693947373738054E-4	0.0	0.0	13.125450394326547	0.0
31	2.693947373738054E-4	0.0	0.0	13.381846835622067	0.0
32	2.693947373738054E-4	0.0	0.0	13.69959792835447	0.0
33	2.693947373738054E-4	0.0	0.0	14.02879829742526	0.0
34	2.693947373738054E-4	0.0	0.0	14.473232265407695	0.0
35	2.693947373738054E-4	0.0	0.0	14.769364430465851	0.0
36	3.3674342171725674E-4	0.0	0.0	15.089540075834618	0.0
37	3.3674342171725674E-4	0.0	0.0	15.548790754372613	0.0
38	3.3674342171725674E-4	0.0	0.0	16.706581986920884	0.0
39	3.3674342171725674E-4	0.0	0.0	17.116196685097755	0.0
40	3.3674342171725674E-4	0.0	0.0	17.45233396865592	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	425	0.0	46.000004	27
TGATCGA	20	6.3120533E-4	46.0	16
CGCGATA	20	6.3120533E-4	46.0	10
TCGGCGA	20	6.3120533E-4	46.0	41
ATCGTAC	20	6.3120533E-4	46.0	45
CGTAGAT	30	1.8617739E-6	46.0	29
CCGCGAT	20	6.3120533E-4	46.0	9
TCGAATC	20	6.3120533E-4	46.0	40
TCACGAC	440	0.0	45.477272	24
CACGACG	420	0.0	45.45238	25
GACGGTC	450	0.0	43.955555	28
CTAGCGG	330	0.0	43.909092	1
TAGTAGG	430	0.0	43.32558	1
CGGTCTA	445	0.0	42.898876	30
GTTACGG	120	0.0	42.166668	1
TACGGGT	165	0.0	41.81818	3
CGCACTT	5960	0.0	41.793625	34
CACTTAC	5970	0.0	41.646564	36
CCCGCAC	6035	0.0	41.617233	32
CCGCACT	5995	0.0	41.549625	33
>>END_MODULE