FastQCFastQC Report
Sat 14 Jan 2017
SRR2933099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090800
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC117301.0753575357535754No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80500.737990465713238No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG60040.5504217088375505No Hit
GGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG15510.14218921892189218No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA14780.13549688302163548No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT14500.13292995966263293Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT14490.13283828382838284Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT11600.10634396773010633Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.10203520352035204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG351.0195072E-746.0000041
TATCTCG351.0195072E-746.00000441
GATACCG206.3113135E-446.016
ACACGAT206.3113135E-446.034
ATTAGGC206.3113135E-446.033
CGAAATT206.3113135E-446.031
CAACGTA206.3113135E-446.032
CGCATTG405.6115823E-946.012
CGGTTAT206.3113135E-446.010
ATTACGA301.8613155E-646.026
CACGATT206.3113135E-446.035
CTGCGAT253.4170414E-546.033
TAACCGA301.8613155E-646.026
CGGATTA253.4170414E-546.037
TATTCGC301.8613155E-646.022
GTCGTGA253.4170414E-546.022
GCGATCA253.4170414E-546.035
GCGATAA600.046.09
CGTCGTT253.4170414E-546.044
AATTACG206.3113135E-446.01
Produced by FastQC (version 0.11.5)