##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090800
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.520027502750274	33.0	31.0	34.0	31.0	34.0
2	32.9192968463513	34.0	31.0	34.0	31.0	34.0
3	32.98566098276494	34.0	31.0	34.0	31.0	34.0
4	36.404528786211955	37.0	37.0	37.0	35.0	37.0
5	36.31338375504217	37.0	37.0	37.0	35.0	37.0
6	36.39015126512651	37.0	37.0	37.0	35.0	37.0
7	36.541729922992296	37.0	37.0	37.0	35.0	37.0
8	36.48780161349468	37.0	37.0	37.0	35.0	37.0
9	38.317015034836814	39.0	39.0	39.0	37.0	39.0
10	38.034256508984235	39.0	39.0	39.0	35.0	39.0
11	37.76667675100843	39.0	38.0	39.0	35.0	39.0
12	37.355446461312795	39.0	37.0	39.0	35.0	39.0
13	37.24212962962963	39.0	37.0	39.0	35.0	39.0
14	38.20991474147415	40.0	37.0	41.0	33.0	41.0
15	38.41018701870187	40.0	37.0	41.0	33.0	41.0
16	38.595174184085074	41.0	37.0	41.0	35.0	41.0
17	38.60595709570957	40.0	37.0	41.0	35.0	41.0
18	38.53114136413642	40.0	37.0	41.0	35.0	41.0
19	38.50024202420242	40.0	36.0	41.0	35.0	41.0
20	38.377214888155486	40.0	35.0	41.0	34.0	41.0
21	38.32786762009534	40.0	35.0	41.0	35.0	41.0
22	38.25109552621929	40.0	35.0	41.0	34.0	41.0
23	38.232641180784746	40.0	35.0	41.0	34.0	41.0
24	38.19760726072607	40.0	35.0	41.0	34.0	41.0
25	38.087495416208284	40.0	35.0	41.0	34.0	41.0
26	37.96440410707737	40.0	35.0	41.0	34.0	41.0
27	37.85730748074808	40.0	35.0	41.0	34.0	41.0
28	37.85536303630363	40.0	35.0	41.0	34.0	41.0
29	37.8231967363403	40.0	35.0	41.0	34.0	41.0
30	37.795138430509716	40.0	35.0	41.0	34.0	41.0
31	37.68984506784012	40.0	35.0	41.0	34.0	41.0
32	37.582506417308394	40.0	35.0	41.0	34.0	41.0
33	37.476000183351665	40.0	35.0	41.0	33.0	41.0
34	37.321581408140815	40.0	35.0	41.0	33.0	41.0
35	37.22215071507151	40.0	35.0	41.0	33.0	41.0
36	37.155841584158416	40.0	35.0	41.0	33.0	41.0
37	37.03395856252292	40.0	35.0	41.0	33.0	41.0
38	37.00643839383938	40.0	35.0	41.0	33.0	41.0
39	36.956963696369634	40.0	35.0	41.0	33.0	41.0
40	36.891499816648334	40.0	35.0	41.0	33.0	41.0
41	36.76971672167217	39.0	35.0	41.0	32.0	41.0
42	36.77834800146681	39.0	35.0	41.0	32.0	41.0
43	36.73739457279061	39.0	35.0	41.0	32.0	41.0
44	36.70371928859553	39.0	35.0	41.0	32.0	41.0
45	36.64073432343234	39.0	35.0	41.0	32.0	41.0
46	36.574017235056836	39.0	35.0	41.0	32.0	41.0
47	36.479867070040335	39.0	35.0	41.0	32.0	41.0
48	36.36636046938027	38.0	35.0	41.0	32.0	41.0
49	36.3216804180418	38.0	35.0	41.0	31.0	41.0
50	36.207891455812245	38.0	35.0	41.0	31.0	41.0
51	36.120980014668135	38.0	35.0	41.0	31.0	41.0
52	35.86369086908691	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	5.0
14	9.0
15	16.0
16	55.0
17	113.0
18	230.0
19	392.0
20	716.0
21	1192.0
22	1935.0
23	2993.0
24	4671.0
25	6919.0
26	9002.0
27	10012.0
28	10701.0
29	11641.0
30	13314.0
31	15952.0
32	20491.0
33	29132.0
34	77309.0
35	121067.0
36	59048.0
37	78075.0
38	130688.0
39	482056.0
40	3062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.99193252658599	22.244682801613493	26.175559222588923	12.587825449211588
2	36.60973597359736	23.554822148881556	25.834250091675838	14.001191785845252
3	33.37788778877888	23.83874220755409	29.03171983865053	13.751650165016502
4	29.215438210487715	27.108452511917857	28.708470847084712	14.967638430509716
5	26.045287862119544	32.00889255592226	26.426017601760176	15.51980198019802
6	29.237807114044738	38.80436376971031	23.248533186651997	8.70929592959296
7	88.71883021635497	1.9317015034836817	7.4159332599926655	1.9335350201686836
8	86.58489182251559	2.445086175284195	7.937660432709938	3.0323615694902823
9	80.77631096442978	4.522827282728273	10.385863586358637	4.314998166483315
10	46.07636596993033	26.579941327466077	15.357810781078108	11.985881921525486
11	33.889255592225894	22.953612027869454	25.93848551521819	17.21864686468647
12	32.77997799779978	19.18967730106344	29.830858085808583	18.1994866153282
13	30.894481114778145	21.43976897689769	30.51567656765676	17.150073340667397
14	20.93060139347268	23.11927026035937	35.00898423175651	20.941144114411443
15	17.500091675834252	27.338008800880086	36.12156215621562	19.04033736707004
16	28.42125045837917	24.615236523652364	30.248533186652	16.714979831316466
17	26.88201320132013	23.12981298129813	27.395764576457648	22.59240924092409
18	23.482398239823983	22.683626696002936	33.37752108544188	20.456453978731208
19	23.408140814081406	24.875779244591126	28.16905023835717	23.547029702970296
20	21.303355335533553	32.19444444444444	27.41070773744041	19.09149248258159
21	25.787953795379536	25.874587458745875	31.493582691602494	16.843876054272094
22	22.90942427576091	22.71140447378071	33.35973597359736	21.01943527686102
23	23.176292629262925	22.37962962962963	32.973230656398975	21.47084708470847
24	23.353868720205355	24.61853685368537	30.07874954162083	21.94884488448845
25	24.466629996332966	23.783461679501283	30.148514851485146	21.601393472680602
26	21.520352035203523	27.074990832416574	27.413274660799413	23.99138247158049
27	23.522827282728272	24.611844517785112	30.502016868353504	21.363311331133115
28	19.244591125779245	28.3506600660066	27.686010267693433	24.718738540520718
29	21.320040337367068	27.09259259259259	27.921525485881922	23.665841584158414
30	24.308030803080307	24.635863586358635	28.10276861019435	22.9533370003667
31	23.20966263292996	26.195636230289697	28.902365236523654	21.692335900256694
32	25.841309130913093	24.785295196186286	26.703337000366705	22.67005867253392
33	25.268243491015767	25.851485148514854	27.699578291162453	21.18069306930693
34	21.359644297763108	24.88879721305464	30.194902823615692	23.556655665566556
35	21.747799779977996	28.224422442244222	27.825265859919323	22.202511917858452
36	24.625595892922625	27.11175284195086	25.962229556288964	22.30042170883755
37	22.18986065273194	28.52805280528053	26.507609094242756	22.774477447744772
38	23.682526585991933	26.804455445544555	27.729281261459484	21.783736707004035
39	22.002658599193254	28.04400440044004	26.16052438577191	23.792812614594794
40	26.001100110011	24.914283094976163	28.17601760176018	20.90859919325266
41	22.252108544187752	24.77860286028603	26.95819581958196	26.01109277594426
42	20.806288962229555	26.298679867986795	29.913091309130913	22.98193986065273
43	20.776585991932524	25.87119545287862	29.601576824349102	23.75064173083975
44	22.645031169783643	24.80812247891456	27.334983498349835	25.21186285295196
45	20.95782911624496	24.943344334433444	26.52933626696003	27.56949028236157
46	23.621470480381372	24.196461312797947	27.90474880821415	24.27731939860653
47	19.905207187385404	24.666299963329667	30.68885221855519	24.73964063072974
48	20.67803447011368	26.99028236156949	28.711587825449215	23.62009534286762
49	24.096076274294095	24.515951595159517	29.2033370003667	22.184635130179682
50	23.062339567290064	24.983590025669233	28.33901723505684	23.615053171983867
51	21.608635863586358	25.213879721305467	27.0490465713238	26.12843784378438
52	21.92106710671067	26.250275027502752	28.585900256692337	23.24275760909424
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	701.0
1	2259.5
2	3818.0
3	7611.0
4	11404.0
5	7165.5
6	2927.0
7	3040.0
8	3153.0
9	3784.5
10	4416.0
11	5204.0
12	5992.0
13	5928.0
14	5628.5
15	5393.0
16	5139.5
17	4886.0
18	4709.5
19	4533.0
20	4424.0
21	4315.0
22	4737.0
23	5159.0
24	5188.5
25	5218.0
26	6213.5
27	7209.0
28	7739.0
29	8269.0
30	11024.0
31	13779.0
32	14703.0
33	15627.0
34	18583.0
35	21539.0
36	24990.5
37	28442.0
38	31314.0
39	35952.5
40	37719.0
41	43227.5
42	48736.0
43	55496.0
44	62256.0
45	71277.0
46	80298.0
47	85018.0
48	89738.0
49	90128.5
50	90519.0
51	79853.5
52	69188.0
53	67710.5
54	66233.0
55	64373.5
56	62514.0
57	62583.5
58	62653.0
59	66107.0
60	69561.0
61	58088.5
62	46616.0
63	40921.5
64	29812.0
65	24397.0
66	20626.5
67	16856.0
68	14463.0
69	12070.0
70	9284.5
71	6499.0
72	5469.0
73	4439.0
74	3940.5
75	3442.0
76	2928.0
77	2414.0
78	1888.5
79	1363.0
80	997.0
81	631.0
82	443.5
83	256.0
84	198.5
85	141.0
86	136.5
87	132.0
88	93.5
89	31.0
90	7.0
91	6.5
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1090800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.7755303306906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.02002478648765	14.44009714912966
2	10.904806749251717	4.312966732402939
3	3.977651486204211	2.3598050283104377
4	1.9354971876300413	1.5310193335577693
5	1.0924578191578709	1.0801966368878293
6	0.7655767701026711	0.908381198258251
7	0.5781993222163935	0.8003938763572527
8	0.4827629721177157	0.7637515038118582
9	0.3540232700044481	0.6300898122368909
>10	4.133763391110803	20.400267534067137
>50	1.9036431387213324	27.31257043920086
>100	0.8404307724414349	21.38640809599102
>500	0.006511361823010563	0.8499451547376089
>1k	0.0032556809115052814	0.8526124428544134
>5k	9.301945461443662E-4	1.292623006674857
>10k+	4.650972730721831E-4	1.0788720555212803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	11730	1.0753575357535754	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8050	0.737990465713238	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6004	0.5504217088375505	No Hit
GGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAG	1551	0.14218921892189218	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1478	0.13549688302163548	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	1450	0.13292995966263293	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT	1449	0.13283828382838284	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	1160	0.10634396773010633	Illumina Single End Adapter 1 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.10203520352035204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.167583425009168E-5	0.0	0.0	0.13586358635863585	0.0
2	9.167583425009168E-5	0.0	0.0	0.46076274294096076	0.0
3	9.167583425009168E-5	0.0	0.0	0.7137880454712138	0.0
4	9.167583425009168E-5	0.0	0.0	1.0070590392372571	0.0
5	9.167583425009168E-5	0.0	0.0	1.8942060872753943	0.0
6	1.8335166850018335E-4	0.0	0.0	2.6105610561056105	0.0
7	1.8335166850018335E-4	0.0	0.0	3.173725705903924	0.0
8	1.8335166850018335E-4	0.0	0.0	4.251558489182251	0.0
9	1.8335166850018335E-4	0.0	0.0	4.7757609094242754	0.0
10	1.8335166850018335E-4	0.0	0.0	5.4277594426109275	0.0
11	1.8335166850018335E-4	0.0	0.0	6.4836817015034836	0.0
12	2.75027502750275E-4	0.0	0.0	7.136780344701137	0.0
13	2.75027502750275E-4	0.0	0.0	7.421342134213421	0.0
14	2.75027502750275E-4	0.0	0.0	7.5504217088375505	0.0
15	2.75027502750275E-4	0.0	0.0	7.763568023469014	0.0
16	2.75027502750275E-4	0.0	0.0	8.15887422075541	0.0
17	2.75027502750275E-4	0.0	0.0	8.576090942427577	0.0
18	2.75027502750275E-4	0.0	0.0	9.027961129446277	0.0
19	2.75027502750275E-4	0.0	0.0	9.316923359002567	0.0
20	2.75027502750275E-4	0.0	0.0	9.72992299229923	0.0
21	3.667033370003667E-4	0.0	0.0	10.117345067840118	0.0
22	3.667033370003667E-4	0.0	0.0	10.54088742207554	0.0
23	3.667033370003667E-4	0.0	0.0	10.912266226622663	0.0
24	3.667033370003667E-4	0.0	0.0	11.208654198753209	0.0
25	4.583791712504584E-4	0.0	0.0	11.526494316098276	0.0
26	4.583791712504584E-4	0.0	0.0	11.794462779611294	0.0
27	4.583791712504584E-4	0.0	0.0	12.156032269893656	0.0
28	4.583791712504584E-4	0.0	0.0	12.442427576090942	0.0
29	4.583791712504584E-4	0.0	0.0	12.796021268793545	0.0
30	4.583791712504584E-4	0.0	0.0	13.661441144114411	0.0
31	4.583791712504584E-4	0.0	0.0	14.02988632196553	0.0
32	4.583791712504584E-4	0.0	0.0	14.330033003300331	0.0
33	4.583791712504584E-4	0.0	0.0	14.655573890722406	0.0
34	4.583791712504584E-4	0.0	0.0	14.994774477447745	0.0
35	4.583791712504584E-4	0.0	0.0	15.34030069673634	0.0
36	4.583791712504584E-4	0.0	0.0	15.615511551155116	0.0
37	5.5005500550055E-4	0.0	0.0	15.943711037770443	0.0
38	5.5005500550055E-4	0.0	0.0	16.335716905023837	0.0
39	5.5005500550055E-4	0.0	0.0	17.426017601760176	0.0
40	5.5005500550055E-4	0.0	0.0	17.773102310231025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	35	1.0195072E-7	46.000004	1
TATCTCG	35	1.0195072E-7	46.000004	41
GATACCG	20	6.3113135E-4	46.0	16
ACACGAT	20	6.3113135E-4	46.0	34
ATTAGGC	20	6.3113135E-4	46.0	33
CGAAATT	20	6.3113135E-4	46.0	31
CAACGTA	20	6.3113135E-4	46.0	32
CGCATTG	40	5.6115823E-9	46.0	12
CGGTTAT	20	6.3113135E-4	46.0	10
ATTACGA	30	1.8613155E-6	46.0	26
CACGATT	20	6.3113135E-4	46.0	35
CTGCGAT	25	3.4170414E-5	46.0	33
TAACCGA	30	1.8613155E-6	46.0	26
CGGATTA	25	3.4170414E-5	46.0	37
TATTCGC	30	1.8613155E-6	46.0	22
GTCGTGA	25	3.4170414E-5	46.0	22
GCGATCA	25	3.4170414E-5	46.0	35
GCGATAA	60	0.0	46.0	9
CGTCGTT	25	3.4170414E-5	46.0	44
AATTACG	20	6.3113135E-4	46.0	1
>>END_MODULE