FastQCFastQC Report
Sat 14 Jan 2017
SRR2933098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301958
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145131.1147056971115812No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC66610.5116140459216043No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT36540.2806542146520855Illumina Single End Adapter 1 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG36240.2783499928569124No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT33220.2551541601188364TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT26740.20538296934309708TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA22370.17181813852674202No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC17020.13072618317948814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG453.110472E-1046.01
TCGTTGC206.311765E-446.022
GTCGCAT253.417408E-546.042
GATCGCG206.311765E-446.032
CGTGCCA253.417408E-546.021
GACGTAC206.311765E-446.028
TTGTACG301.8615956E-646.01
ATGTCGT405.6115823E-946.041
AATATCG405.6115823E-946.043
ATCGTAG301.8615956E-646.01
CGTTAGC206.311765E-446.011
ACTAGTC206.311765E-446.015
ATATCGT405.6115823E-946.044
CCCGTAA206.311765E-446.030
CTTATCC206.311765E-446.039
ACGGCCG253.417408E-546.026
ATATACG253.417408E-546.01
AACGATT206.311765E-446.017
AATTCGG206.311765E-446.013
TCCCGTA206.311765E-446.029
Produced by FastQC (version 0.11.5)