##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933098.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1301958
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.445370741606105	33.0	31.0	34.0	31.0	34.0
2	32.83522970787076	34.0	31.0	34.0	31.0	34.0
3	32.89762265756653	34.0	31.0	34.0	31.0	34.0
4	36.34596891758413	37.0	37.0	37.0	35.0	37.0
5	36.279946818560965	37.0	37.0	37.0	35.0	37.0
6	36.36975616724963	37.0	37.0	37.0	35.0	37.0
7	36.51987852142696	37.0	37.0	37.0	35.0	37.0
8	36.43984598581521	37.0	37.0	37.0	35.0	37.0
9	38.28271188471518	39.0	39.0	39.0	37.0	39.0
10	37.962284497656604	39.0	38.0	39.0	35.0	39.0
11	37.689141277982856	39.0	37.0	39.0	35.0	39.0
12	37.3712201161635	39.0	37.0	39.0	35.0	39.0
13	37.27232061249288	39.0	37.0	39.0	35.0	39.0
14	38.403171991723234	40.0	38.0	41.0	33.0	41.0
15	38.570569096698975	41.0	38.0	41.0	34.0	41.0
16	38.72047485402755	41.0	38.0	41.0	35.0	41.0
17	38.72967561165567	41.0	38.0	41.0	35.0	41.0
18	38.66848546573699	40.0	37.0	41.0	35.0	41.0
19	38.63659734031359	40.0	37.0	41.0	35.0	41.0
20	38.519262526133716	40.0	36.0	41.0	35.0	41.0
21	38.454484706879946	40.0	36.0	41.0	35.0	41.0
22	38.40503610715553	40.0	36.0	41.0	35.0	41.0
23	38.36141565242504	40.0	36.0	41.0	35.0	41.0
24	38.3009505682979	40.0	35.0	41.0	34.0	41.0
25	38.19697870438217	40.0	35.0	41.0	34.0	41.0
26	38.08636223288309	40.0	35.0	41.0	34.0	41.0
27	37.95852861613047	40.0	35.0	41.0	34.0	41.0
28	37.94536766931038	40.0	35.0	41.0	34.0	41.0
29	37.91387817425754	40.0	35.0	41.0	34.0	41.0
30	37.87738774983525	40.0	35.0	41.0	34.0	41.0
31	37.78583180102584	40.0	35.0	41.0	34.0	41.0
32	37.69197700693878	40.0	35.0	41.0	34.0	41.0
33	37.588570445436794	40.0	35.0	41.0	34.0	41.0
34	37.47541548959337	40.0	35.0	41.0	33.0	41.0
35	37.40672433365746	40.0	35.0	41.0	33.0	41.0
36	37.31871995870834	40.0	35.0	41.0	33.0	41.0
37	37.21843254544309	40.0	35.0	41.0	33.0	41.0
38	37.21712989205489	40.0	35.0	41.0	33.0	41.0
39	37.189831008373545	40.0	35.0	41.0	33.0	41.0
40	37.118586006614656	40.0	35.0	41.0	33.0	41.0
41	37.021029864250615	40.0	35.0	41.0	33.0	41.0
42	37.00189944683316	40.0	35.0	41.0	33.0	41.0
43	36.93169057680816	40.0	35.0	41.0	33.0	41.0
44	36.86595036091794	39.0	35.0	41.0	32.0	41.0
45	36.80064564294701	39.0	35.0	41.0	32.0	41.0
46	36.70314480190605	39.0	35.0	41.0	32.0	41.0
47	36.611182541986764	39.0	35.0	41.0	32.0	41.0
48	36.53506564727894	39.0	35.0	41.0	31.0	41.0
49	36.48946125758281	39.0	35.0	41.0	31.0	41.0
50	36.38742570804895	39.0	35.0	41.0	31.0	41.0
51	36.31941429754262	39.0	35.0	41.0	31.0	41.0
52	36.048678221570896	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	3.0
14	11.0
15	20.0
16	48.0
17	110.0
18	199.0
19	406.0
20	744.0
21	1221.0
22	1927.0
23	3141.0
24	4989.0
25	7184.0
26	9721.0
27	11389.0
28	12604.0
29	14208.0
30	16634.0
31	19599.0
32	24856.0
33	34064.0
34	78987.0
35	142737.0
36	76507.0
37	87978.0
38	149916.0
39	598430.0
40	4322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.58647821204678	23.17609323803072	27.10571308751895	14.131715462403548
2	33.14776667142873	25.20227226991961	27.375614267126895	14.274346791524763
3	30.22079053241349	23.938329807873988	30.69261834867177	15.148261311040756
4	27.21693019283264	28.344309109817672	29.057772984996443	15.380987712353239
5	24.170288135254747	32.43292026317285	27.057094007640796	16.3396975939316
6	27.230064257065127	39.481381119821066	22.605107077186823	10.68344754592698
7	86.72115383138319	1.889077835076093	8.383296542592003	3.0064717909487095
8	83.80001505424907	2.4193560775385996	9.286013834547658	4.49461503366468
9	78.22333746557109	3.949359349533549	12.127887381927835	5.699415802967531
10	44.210565932234374	18.64706849222479	19.295322890600158	17.847042684940682
11	28.348072672083124	25.076999411655372	29.1818937323631	17.393034183898408
12	30.34445043542111	19.889965728541167	29.884681379890903	19.880902456146817
13	26.951791071601388	22.36807946185668	32.40288857244243	18.277240894099503
14	18.206654899774033	24.286497721124643	34.15716943250089	23.349677946600426
15	14.468208651891997	29.506251353730306	36.638662691116	19.386877303261702
16	22.227061087992087	27.09311667503867	32.307801019694956	18.37202121727429
17	21.086164069808703	28.575038518907675	28.688713460802884	21.650083950480735
18	18.195441020370858	26.94464798403635	34.336745117738054	20.523165877854737
19	19.562766233626583	28.559830655059532	29.80103812872612	22.076364982587766
20	17.454710520615873	32.68661508282141	29.786060687057493	20.072613709505223
21	20.424775607200846	29.785446227912114	32.02599469414528	17.76378347074176
22	19.97890868983485	25.196818945004367	31.817309006895766	23.00696335826501
23	19.490413669258146	25.79660787828793	32.30173323563433	22.41124521681959
24	18.372328446846982	27.442359891793743	32.98470457572364	21.200607085635635
25	20.590986806026002	27.425846302261668	30.10396648739821	21.879200404314116
26	19.087942929034575	29.30570725015707	28.22233896945984	23.384010851348506
27	20.912579361238993	27.627696131518835	31.50109296920484	19.958631538037324
28	18.534699276013512	28.52104292150745	29.503793517148786	23.440464285330247
29	20.106485769894267	26.496860881841044	29.804187231846193	23.5924661164185
30	23.098671385712905	26.113207952944716	29.56370328382329	21.22441737751909
31	21.729502795021038	26.58849210189576	30.019631969694878	21.66237313338833
32	24.577751356034526	25.237603670778935	28.26104989561875	21.923595077567786
33	23.242531633124877	24.33934120762728	30.83824516612671	21.57988199312113
34	19.504008577849667	23.381322592587473	33.50661081233035	23.608058017232505
35	21.583799170172924	25.410343498023746	29.470612723298295	23.53524460850504
36	21.83726356764197	25.00464684728693	30.29337351896144	22.864716066109658
37	19.39002640638177	25.90198762172052	31.01943380662049	23.688552165277223
38	20.795678508830544	24.74680442840706	30.679253862259763	23.77826320050263
39	20.053719090784803	25.875105034110163	29.208008246041732	24.863167629063305
40	22.42330397754766	24.64150149236765	29.380517651107024	23.554676878977663
41	18.635240153676232	24.420142585244687	29.25217249711588	27.6924447639632
42	19.099924882369475	25.153038730896082	30.75782782547517	24.98920856125927
43	20.653738446247882	25.982558577158404	31.540802391474994	21.82290058511872
44	21.901935392693158	25.984478761987713	28.810299564194853	23.303286281124276
45	20.342130852147307	25.75866502606075	28.564669520829394	25.33453460096255
46	22.19879596730463	25.54598535436627	28.971364667677452	23.283854010651652
47	19.846415936612395	24.498332511494226	31.87683473660441	23.77841681528897
48	18.78639710343959	24.40800701712344	32.196660721774435	24.608935157662536
49	23.261810288811162	23.638857781894654	29.985299064946798	23.114032864347394
50	20.107407458612336	23.602988729283126	31.811394837621492	24.47820897448305
51	18.96635682564261	23.5356286454709	30.004270491060385	27.493744037826108
52	19.31245093927761	24.609011965055707	31.46092270257566	24.61761439309102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	685.0
1	2677.5
2	4670.0
3	11389.0
4	18108.0
5	10705.5
6	3303.0
7	3751.5
8	4200.0
9	5536.5
10	6873.0
11	8243.5
12	9614.0
13	9918.5
14	9968.0
15	9713.0
16	8612.0
17	7511.0
18	7143.5
19	6776.0
20	6309.0
21	5842.0
22	6256.5
23	6671.0
24	7089.5
25	7508.0
26	9550.5
27	11593.0
28	13570.0
29	15547.0
30	16901.5
31	18256.0
32	20946.0
33	23636.0
34	27291.0
35	30946.0
36	32192.0
37	33438.0
38	35604.0
39	42843.5
40	47917.0
41	54256.5
42	60596.0
43	65682.5
44	70769.0
45	75272.5
46	79776.0
47	90775.5
48	101775.0
49	119084.5
50	136394.0
51	134373.5
52	132353.0
53	120061.0
54	107769.0
55	95113.5
56	82458.0
57	72755.5
58	63053.0
59	58109.5
60	53166.0
61	44032.0
62	34898.0
63	28545.0
64	17743.0
65	13294.0
66	11038.0
67	8782.0
68	7205.0
69	5628.0
70	4175.0
71	2722.0
72	2543.5
73	2365.0
74	1895.5
75	1426.0
76	1100.5
77	775.0
78	660.0
79	545.0
80	355.5
81	166.0
82	137.0
83	108.0
84	94.0
85	80.0
86	67.0
87	54.0
88	31.0
89	5.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1301958.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.18183086652333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.96486117220346	14.120189947771955
2	12.1760305802672	4.914691795931372
3	5.124280665726199	3.102520971248452
4	2.6879603662088503	2.1699184594698053
5	1.5666576238727956	1.5809009595375048
6	1.0333293062163258	1.2512686372487867
7	0.6642710064192796	0.9384343570762393
8	0.524744094320546	0.8472237247827381
9	0.38935224946151137	0.7072057121519361
>10	3.3703660710418673	14.986605620972195
>50	1.3713670235591866	20.329435081317516
>100	1.1141879901293785	30.67385052781834
>500	0.008394567048919947	1.152271604357664
>1k	0.003434141065467251	1.594913934227867
>5k	3.8157122949636113E-4	0.5129506888074852
>10k+	3.8157122949636113E-4	1.117617977280143
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14513	1.1147056971115812	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	6661	0.5116140459216043	No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCTT	3654	0.2806542146520855	Illumina Single End Adapter 1 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	3624	0.2783499928569124	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	3322	0.2551541601188364	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT	2674	0.20538296934309708	TruSeq Adapter, Index 13 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2237	0.17181813852674202	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC	1702	0.13072618317948814	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.292328938414296	0.0
2	0.0	0.0	0.0	0.9064808542211039	0.0
3	0.0	0.0	0.0	1.3764652930432473	0.0
4	0.0	0.0	0.0	1.9114287864892723	0.0
5	0.0	0.0	0.0	3.3841337431775833	0.0
6	0.0	0.0	0.0	4.8435510208470625	0.0
7	0.0	0.0	0.0	6.015862262837972	0.0
8	0.0	0.0	0.0	8.16885030085456	0.0
9	0.0	0.0	0.0	9.285399375402278	0.0
10	0.0	0.0	0.0	10.41024364841262	0.0
11	0.0	0.0	0.0	12.257077417243874	0.0
12	0.0	0.0	0.0	13.26448318609356	0.0
13	0.0	0.0	0.0	13.68300667149017	0.0
14	0.0	0.0	0.0	13.858434757496017	0.0
15	0.0	0.0	0.0	14.15445045078259	0.0
16	0.0	0.0	0.0	14.743025504662977	0.0
17	7.68073931724372E-5	0.0	0.0	15.380526867994206	0.0
18	7.68073931724372E-5	0.0	0.0	15.993065828544392	0.0
19	7.68073931724372E-5	0.0	0.0	16.397533560990446	0.0
20	7.68073931724372E-5	0.0	0.0	16.874507472591283	0.0
21	7.68073931724372E-5	0.0	0.0	17.409470966037308	0.0
22	7.68073931724372E-5	0.0	0.0	17.96202335251982	0.0
23	7.68073931724372E-5	0.0	0.0	18.492685631948188	0.0
24	1.536147863448744E-4	0.0	0.0	18.874111146442512	0.0
25	1.536147863448744E-4	0.0	0.0	19.2768123088456	0.0
26	1.536147863448744E-4	0.0	0.0	19.62720763649826	0.0
27	1.536147863448744E-4	0.0	0.0	20.01393286112148	0.0
28	1.536147863448744E-4	0.0	0.0	20.34136277821558	0.0
29	1.536147863448744E-4	0.0	0.0	20.727243121513904	0.0
30	1.536147863448744E-4	0.0	0.0	21.37664963078686	0.0
31	1.536147863448744E-4	0.0	0.0	21.760072137503666	0.0
32	1.536147863448744E-4	0.0	0.0	22.05946735608983	0.0
33	1.536147863448744E-4	0.0	0.0	22.394424397714825	0.0
34	1.536147863448744E-4	0.0	0.0	22.75695529348873	0.0
35	1.536147863448744E-4	0.0	0.0	23.116413893535736	0.0
36	1.536147863448744E-4	0.0	0.0	23.42786787284997	0.0
37	1.536147863448744E-4	0.0	0.0	23.784561406742768	0.0
38	1.536147863448744E-4	0.0	0.0	24.16982729089571	0.0
39	1.536147863448744E-4	0.0	0.0	24.980068481471754	0.0
40	1.536147863448744E-4	0.0	0.0	25.31932673711441	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	45	3.110472E-10	46.0	1
TCGTTGC	20	6.311765E-4	46.0	22
GTCGCAT	25	3.417408E-5	46.0	42
GATCGCG	20	6.311765E-4	46.0	32
CGTGCCA	25	3.417408E-5	46.0	21
GACGTAC	20	6.311765E-4	46.0	28
TTGTACG	30	1.8615956E-6	46.0	1
ATGTCGT	40	5.6115823E-9	46.0	41
AATATCG	40	5.6115823E-9	46.0	43
ATCGTAG	30	1.8615956E-6	46.0	1
CGTTAGC	20	6.311765E-4	46.0	11
ACTAGTC	20	6.311765E-4	46.0	15
ATATCGT	40	5.6115823E-9	46.0	44
CCCGTAA	20	6.311765E-4	46.0	30
CTTATCC	20	6.311765E-4	46.0	39
ACGGCCG	25	3.417408E-5	46.0	26
ATATACG	25	3.417408E-5	46.0	1
AACGATT	20	6.311765E-4	46.0	17
AATTCGG	20	6.311765E-4	46.0	13
TCCCGTA	20	6.311765E-4	46.0	29
>>END_MODULE