FastQCFastQC Report
Sat 14 Jan 2017
SRR2933096.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933096.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556274
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC282081.8125342966598426No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241471.5515905296882169No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG134560.864629236239891No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA33180.21320153134987796No Hit
GGGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT32300.20754700007839236TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT28300.1818445852080032Illumina PCR Primer Index 3 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT24170.15530684185432642TruSeq Adapter, Index 14 (96% over 26bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCG23110.1484957019136733No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG21330.1370581272963501No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG20060.12889761057500157No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC19980.1283835622775938Illumina PCR Primer Index 3 (95% over 23bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCATTGCGTCT19170.12317882326634No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT18370.11803834029226216TruSeq Adapter, Index 14 (96% over 26bp)
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15640.10049644214322156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAC206.312146E-446.026
AGCGTTA206.312146E-446.010
AGCCGTA206.312146E-446.013
AGCGTAC206.312146E-446.041
ACTATCG253.4177174E-546.045
ACCGGTA501.6370905E-1146.041
ACCGGAA206.312146E-446.032
CCGATTA206.312146E-446.012
CGAAAGT253.4177174E-546.016
GCGAATC206.312146E-446.08
CGTTGAT253.4177174E-546.025
ATTCGCG206.312146E-446.012
TACGGTG206.312146E-446.043
TGCGGTA206.312146E-446.031
TACGGAT206.312146E-446.011
CGCGAAT301.8618302E-646.034
TATTGCG551.8189894E-1246.01
ATATCCG253.4177174E-546.044
TAATTCG253.4177174E-546.014
CGAATCG206.312146E-446.09
Produced by FastQC (version 0.11.5)