##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1556274
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49173410337768	33.0	31.0	34.0	31.0	34.0
2	32.87547758299631	34.0	31.0	34.0	31.0	34.0
3	32.899089106416994	34.0	31.0	34.0	31.0	34.0
4	36.3423580937547	37.0	37.0	37.0	35.0	37.0
5	36.214927448508426	37.0	37.0	37.0	35.0	37.0
6	36.30653149766686	37.0	37.0	37.0	35.0	37.0
7	36.52710512416194	37.0	37.0	37.0	35.0	37.0
8	36.48329278777388	37.0	37.0	37.0	35.0	37.0
9	38.31810465252263	39.0	39.0	39.0	37.0	39.0
10	38.03734111088407	39.0	39.0	39.0	35.0	39.0
11	37.687508754885066	39.0	37.0	39.0	35.0	39.0
12	37.247707023313374	39.0	35.0	39.0	35.0	39.0
13	37.13528723091178	39.0	35.0	39.0	34.0	39.0
14	37.810467822504265	40.0	36.0	41.0	32.0	41.0
15	38.1617215220456	40.0	36.0	41.0	33.0	41.0
16	38.41960541652691	40.0	36.0	41.0	34.0	41.0
17	38.47641417899419	40.0	36.0	41.0	35.0	41.0
18	38.42364390846342	40.0	36.0	41.0	35.0	41.0
19	38.355703430115774	40.0	36.0	41.0	35.0	41.0
20	38.179044949668246	40.0	35.0	41.0	34.0	41.0
21	38.145575907584394	40.0	35.0	41.0	34.0	41.0
22	38.101392171301455	40.0	35.0	41.0	34.0	41.0
23	38.06987137226478	40.0	35.0	41.0	34.0	41.0
24	38.0145803373956	40.0	35.0	41.0	34.0	41.0
25	37.863254157044324	40.0	35.0	41.0	34.0	41.0
26	37.77736182703046	40.0	35.0	41.0	34.0	41.0
27	37.66567712369416	40.0	35.0	41.0	34.0	41.0
28	37.654886607371196	40.0	35.0	41.0	34.0	41.0
29	37.59569266080395	40.0	35.0	41.0	34.0	41.0
30	37.54099792195976	40.0	35.0	41.0	34.0	41.0
31	37.403090972412315	40.0	35.0	41.0	33.0	41.0
32	37.28230697165152	40.0	35.0	41.0	33.0	41.0
33	37.177749547958776	40.0	35.0	41.0	33.0	41.0
34	37.009029258343965	39.0	35.0	41.0	33.0	41.0
35	36.90230255083616	39.0	35.0	41.0	33.0	41.0
36	36.82226523092977	39.0	35.0	41.0	33.0	41.0
37	36.704034765086355	39.0	35.0	41.0	32.0	41.0
38	36.66206272160301	39.0	35.0	41.0	32.0	41.0
39	36.61853953738223	39.0	35.0	41.0	32.0	41.0
40	36.52768921153987	39.0	35.0	41.0	32.0	41.0
41	36.33951476410966	39.0	35.0	41.0	31.0	41.0
42	36.36643354576379	39.0	35.0	41.0	31.0	41.0
43	36.31143873122599	38.0	35.0	41.0	31.0	41.0
44	36.27283884457364	38.0	35.0	41.0	31.0	41.0
45	36.20033618758651	38.0	35.0	41.0	31.0	41.0
46	36.13179234504978	38.0	35.0	41.0	31.0	41.0
47	36.038711049596664	38.0	35.0	41.0	31.0	41.0
48	35.89989166432132	37.0	35.0	41.0	31.0	41.0
49	35.86685763560915	37.0	35.0	41.0	31.0	41.0
50	35.735089707853504	37.0	35.0	40.0	31.0	41.0
51	35.63944973700004	37.0	35.0	40.0	30.0	41.0
52	35.38833136067299	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	3.0
12	4.0
13	8.0
14	7.0
15	32.0
16	60.0
17	174.0
18	316.0
19	596.0
20	1031.0
21	1670.0
22	2721.0
23	4303.0
24	6883.0
25	10730.0
26	13948.0
27	15665.0
28	16645.0
29	18001.0
30	20957.0
31	25797.0
32	32910.0
33	46966.0
34	125824.0
35	180108.0
36	108634.0
37	119972.0
38	184064.0
39	614407.0
40	3835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.33153673453389	22.84835446714396	26.361167763517223	13.458941034804925
2	38.72679232577297	23.48371816273998	24.826348059531934	12.963141451955117
3	34.0046804097479	23.228621695151368	28.9224134053515	13.844284489749233
4	29.339756366809443	26.364958869710602	29.987714245691954	14.307570517788
5	25.45412954274119	31.346922200075305	28.654529986364867	14.544418270818635
6	27.386180068548338	37.459470504551255	25.79892743822746	9.355421988672946
7	86.2906531883203	1.998748292395812	9.264628208143296	2.445970311140583
8	84.49463269321468	2.065510315021648	9.692380647623748	3.7474763441399133
9	78.75611878114009	3.9272004801211096	12.357785325720277	4.9588954130185305
10	49.911069644548455	21.665272310659947	16.950871119095996	11.472786925695603
11	34.158830642933054	23.735473316395442	26.580666386510345	15.525029654161157
12	32.63281401604088	18.560099314131058	31.083986495951226	17.723100173876837
13	29.487480996277004	20.180251035486037	34.30610548014039	16.026162488096567
14	18.76160624671491	23.01876147773464	39.188343440807984	19.03128883474247
15	15.691838326669982	28.251516121197167	38.795417773476906	17.261227778655943
16	28.059711850226886	25.345151303690738	30.892889041389882	15.70224780469249
17	26.082810610470908	24.169394335444785	27.020306192868347	22.727488861215956
18	23.492971032093322	24.395704098378562	33.72034744524422	18.3909774242839
19	23.293327524587575	26.160560415453833	27.85769086934563	22.688421190612964
20	18.41198914844044	37.42747099803762	25.820967258978815	18.33957259454312
21	23.152221266949134	27.47633128870623	33.294908223102105	16.07653922124253
22	19.947901205057722	23.701803152915232	36.1037323761754	20.246563265851645
23	21.225054199967357	24.847873831985883	34.27307787703194	19.65399409101482
24	21.81261140390445	29.519159222604756	29.13696431348207	19.531265060008714
25	23.80956052725934	25.66533913693861	30.357893275862736	20.167207059939315
26	18.876560297222724	29.632442616145998	26.720101987182204	24.77089509944907
27	22.34208115023447	26.578738705395068	31.262361255151728	19.816818889218737
28	17.941056651977735	29.70884304434823	27.203114618633993	25.146985685040036
29	21.86562263457463	30.47952995423685	26.117380358471582	21.537467052716938
30	25.462675595685592	25.53496363750856	26.79406068597175	22.208300080834096
31	22.814298767440693	26.28283965419971	31.589617252488956	19.313244325870638
32	27.01587252630321	25.215161340483743	26.414371762298927	21.35459437091412
33	26.079919088797986	24.168944543184555	29.600764389818245	20.15037197819921
34	20.300602593116636	24.078664810952315	34.13550570143818	21.48522689449287
35	22.60707304754818	29.84294539393449	27.89405978638723	19.6559217721301
36	26.105235967445324	25.120447941686365	26.477021398545503	22.297294692322815
37	21.69367347909173	28.27060016423843	27.69081794079963	22.34490841587021
38	24.174663330493214	25.016738697684342	30.146105377330727	20.662492594491717
39	21.635200485261592	26.99543910648125	25.438836605893307	25.930523802363854
40	27.286968747148638	24.251513550955682	28.089012603179132	20.37250509871655
41	20.92504276239274	24.96173552986171	26.934652895312777	27.178568812432776
42	19.07067778553134	27.48892547199272	31.07370553000307	22.366691212472865
43	19.463089404565007	27.097991741814102	31.552027470741013	21.886891382879877
44	24.313327858718967	25.918700691523473	26.891986886627933	22.875984563129627
45	20.361902852582514	25.162278621887918	26.07876248012882	28.39705604540075
46	23.017797637177	24.586673040865552	28.472364120970983	23.92316520098646
47	19.950085910321704	25.00369472213762	31.257927588586586	23.78829177895409
48	19.19578428991296	29.643109118317213	28.291290608209096	22.869815983560734
49	24.485791062499278	25.305055536492933	28.273363173836998	21.93579022717079
50	21.468391812752767	25.561244356713537	28.429762368323313	24.540601462210383
51	19.987547179995296	26.80774722189023	26.997623811745232	26.20708178636924
52	20.63415568209711	27.335353543142144	28.059197801929482	23.97129297283126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1126.0
1	2852.0
2	4578.0
3	16639.5
4	28701.0
5	16748.0
6	4795.0
7	5452.0
8	6109.0
9	8877.0
10	11645.0
11	13828.0
12	16011.0
13	16565.5
14	16161.0
15	15202.0
16	13568.0
17	11934.0
18	10767.5
19	9601.0
20	8795.5
21	7990.0
22	7640.0
23	7290.0
24	7902.5
25	8515.0
26	9969.5
27	11424.0
28	12200.0
29	12976.0
30	14886.5
31	16797.0
32	18750.5
33	20704.0
34	23233.0
35	25762.0
36	27239.5
37	28717.0
38	32698.0
39	41597.0
40	46515.0
41	51452.5
42	56390.0
43	63041.0
44	69692.0
45	83315.0
46	96938.0
47	115832.0
48	134726.0
49	149229.5
50	163733.0
51	150199.0
52	136665.0
53	123263.5
54	109862.0
55	100863.0
56	91864.0
57	86255.0
58	80646.0
59	87548.0
60	94450.0
61	71364.5
62	48279.0
63	42717.5
64	32494.5
65	27833.0
66	23791.5
67	19750.0
68	16234.0
69	12718.0
70	10932.0
71	9146.0
72	7480.0
73	5814.0
74	5248.0
75	4682.0
76	3634.0
77	2586.0
78	2248.5
79	1911.0
80	1263.0
81	615.0
82	521.5
83	428.0
84	263.0
85	98.0
86	81.0
87	64.0
88	43.5
89	15.5
90	8.0
91	5.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1556274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.767131911121233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7937872507718	15.19210573664281
2	11.686646050486257	5.087695323590366
3	5.008528287531318	3.27063887745829
4	2.784526307526678	2.424446057836821
5	1.7110807992098604	1.8622660733493883
6	1.0833028984283049	1.4148238253873326
7	0.8095625642736172	1.2335298588794572
8	0.6049541431121638	1.0534493306641424
9	0.4479935158446263	0.8776380559245264
>10	4.144495698858937	19.893826878310765
>50	1.196620212958983	18.81106841187743
>100	0.7145950383689136	21.183225936020982
>500	0.007101565597545482	1.1006146917772412
>1k	0.005917971331287903	2.3558745073230427
>5k	0.0	0.0
>10k+	8.876956996931852E-4	4.238796434957399
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	28208	1.8125342966598426	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24147	1.5515905296882169	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	13456	0.864629236239891	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	3318	0.21320153134987796	No Hit
GGGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	3230	0.20754700007839236	TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	2830	0.1818445852080032	Illumina PCR Primer Index 3 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	2417	0.15530684185432642	TruSeq Adapter, Index 14 (96% over 26bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCG	2311	0.1484957019136733	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2133	0.1370581272963501	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCG	2006	0.12889761057500157	No Hit
CGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	1998	0.1283835622775938	Illumina PCR Primer Index 3 (95% over 23bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCATTGCGTCT	1917	0.12317882326634	No Hit
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1837	0.11803834029226216	TruSeq Adapter, Index 14 (96% over 26bp)
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1564	0.10049644214322156	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18865572514865633	0.0
2	0.0	0.0	0.0	0.5657101512972651	0.0
3	0.0	0.0	0.0	0.9362104616539247	0.0
4	0.0	0.0	0.0	1.4006530983618566	0.0
5	0.0	0.0	0.0	2.820390239764977	0.0
6	0.0	0.0	0.0	4.2457819124395835	0.0
7	0.0	0.0	0.0	5.420832064276599	0.0
8	0.0	0.0	0.0	7.388416178642064	0.0
9	0.0	0.0	0.0	8.527418693623359	0.0
10	0.0	0.0	0.0	9.697071338337594	0.0
11	0.0	0.0	0.0	11.39163155074235	0.0
12	0.0	0.0	0.0	12.41600129540171	0.0
13	1.2851207435194575E-4	0.0	0.0	12.874789400838155	0.0
14	1.2851207435194575E-4	0.0	0.0	13.224085218926744	0.0
15	1.2851207435194575E-4	0.0	0.0	13.543823259914385	0.0
16	1.2851207435194575E-4	0.0	0.0	14.042771388585814	0.0
17	1.2851207435194575E-4	0.0	0.0	14.595630332447886	0.0
18	1.2851207435194575E-4	0.0	0.0	15.254318969538783	0.0
19	1.2851207435194575E-4	0.0	0.0	15.662152037494684	0.0
20	1.2851207435194575E-4	0.0	0.0	16.216039077951567	0.0
21	1.2851207435194575E-4	0.0	0.0	16.710746308169384	0.0
22	1.2851207435194575E-4	0.0	0.0	17.26032819413548	0.0
23	1.2851207435194575E-4	0.0	0.0	17.739421207319534	0.0
24	1.2851207435194575E-4	0.0	0.0	18.10760830033786	0.0
25	1.2851207435194575E-4	0.0	0.0	18.50072673578046	0.0
26	1.2851207435194575E-4	0.0	0.0	18.843982486374507	0.0
27	1.2851207435194575E-4	0.0	0.0	19.330143663647917	0.0
28	1.2851207435194575E-4	0.0	0.0	19.70713383375935	0.0
29	1.2851207435194575E-4	0.0	0.0	20.188154528058682	0.0
30	1.2851207435194575E-4	0.0	0.0	21.438898291688997	0.0
31	1.2851207435194575E-4	0.0	0.0	21.96027177733484	0.0
32	1.927681115279186E-4	0.0	0.0	22.32376817963932	0.0
33	1.927681115279186E-4	0.0	0.0	22.748243561223795	0.0
34	1.927681115279186E-4	0.0	0.0	23.22335270010294	0.0
35	1.927681115279186E-4	0.0	0.0	23.688373641145454	0.0
36	1.927681115279186E-4	0.0	0.0	24.033814097003486	0.0
37	1.927681115279186E-4	0.0	0.0	24.46355847363639	0.0
38	2.570241487038915E-4	0.0	0.0	24.97291608033033	0.0
39	2.570241487038915E-4	0.0	0.0	26.737965165517124	0.0
40	2.570241487038915E-4	0.0	0.0	27.19919500036626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAC	20	6.312146E-4	46.0	26
AGCGTTA	20	6.312146E-4	46.0	10
AGCCGTA	20	6.312146E-4	46.0	13
AGCGTAC	20	6.312146E-4	46.0	41
ACTATCG	25	3.4177174E-5	46.0	45
ACCGGTA	50	1.6370905E-11	46.0	41
ACCGGAA	20	6.312146E-4	46.0	32
CCGATTA	20	6.312146E-4	46.0	12
CGAAAGT	25	3.4177174E-5	46.0	16
GCGAATC	20	6.312146E-4	46.0	8
CGTTGAT	25	3.4177174E-5	46.0	25
ATTCGCG	20	6.312146E-4	46.0	12
TACGGTG	20	6.312146E-4	46.0	43
TGCGGTA	20	6.312146E-4	46.0	31
TACGGAT	20	6.312146E-4	46.0	11
CGCGAAT	30	1.8618302E-6	46.0	34
TATTGCG	55	1.8189894E-12	46.0	1
ATATCCG	25	3.4177174E-5	46.0	44
TAATTCG	25	3.4177174E-5	46.0	14
CGAATCG	20	6.312146E-4	46.0	9
>>END_MODULE