FastQCFastQC Report
Sat 14 Jan 2017
SRR2933095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026819
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154601.5056207569201583No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC120351.1720663524925035No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG64320.6264005632930438No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT63070.6142270448832754Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT62030.6040986775663482Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT58640.5710840956390562Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT26850.2614871754418257Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC26630.25934463620170645Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA22240.21659123954659976No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG21230.20675503667150685No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT19710.19195203828522847Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC18600.1811419539373541Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA16010.1559184237923139No Hit
GGGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT15830.1541654371413073No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGATTATGCTCG15010.1461796090644992No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC13780.13420086694928707Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC12650.12319600630685641Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC12550.12222212483407494Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC10600.10323143611483619Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGATTATGCT10550.10274449537844549No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGAT206.3111365E-446.00000420
TCACGAT206.3111365E-446.00000413
CGGGTGG405.6097633E-946.0000046
AACGTCA206.3111365E-446.00000435
CTATGCG206.3111365E-446.0000041
CAGCGCG206.3111365E-446.0000041
TCGTTGA1550.046.00000424
GGTACCG206.3111365E-446.00000436
TCTTGAC206.3111365E-446.00000432
CGAACGT206.3111365E-446.00000433
ACGCCTA206.3111365E-446.00000430
TCCGCTC405.6097633E-946.00000438
CTACTTA206.3111365E-446.00000431
GCGACGT206.3111365E-446.00000432
ATGATGT206.3111365E-446.00000429
ACGCCCT206.3111365E-446.00000421
CTACTGA206.3111365E-446.00000419
TTAATCG1550.046.00000420
CCGATAC206.3111365E-446.00000444
TCCGAAC206.3111365E-446.00000431
Produced by FastQC (version 0.11.5)