##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933095.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1026819
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28899640540348	33.0	31.0	34.0	31.0	34.0
2	32.63298302816757	34.0	31.0	34.0	31.0	34.0
3	32.665969367532156	34.0	31.0	34.0	31.0	34.0
4	36.15400182505388	37.0	35.0	37.0	35.0	37.0
5	36.03398262011124	37.0	35.0	37.0	35.0	37.0
6	36.13909364746854	37.0	36.0	37.0	35.0	37.0
7	36.44157538962563	37.0	37.0	37.0	35.0	37.0
8	36.3324685265855	37.0	37.0	37.0	35.0	37.0
9	38.1638584794399	39.0	39.0	39.0	37.0	39.0
10	37.85053354096486	39.0	38.0	39.0	35.0	39.0
11	37.55939849184715	39.0	37.0	39.0	35.0	39.0
12	37.20887322887481	39.0	35.0	39.0	35.0	39.0
13	37.097523516802866	39.0	35.0	39.0	33.0	39.0
14	37.88038982527593	40.0	36.0	41.0	33.0	41.0
15	38.185486439187436	40.0	36.0	41.0	33.0	41.0
16	38.42705384298498	40.0	36.0	41.0	34.0	41.0
17	38.48217845598884	40.0	36.0	41.0	35.0	41.0
18	38.487741266961365	40.0	36.0	41.0	35.0	41.0
19	38.46171233683833	40.0	36.0	41.0	35.0	41.0
20	38.30855973642872	40.0	36.0	41.0	34.0	41.0
21	38.25011126595827	40.0	35.0	41.0	34.0	41.0
22	38.218476674077905	40.0	35.0	41.0	34.0	41.0
23	38.21846790914465	40.0	35.0	41.0	34.0	41.0
24	38.14253144906746	40.0	35.0	41.0	34.0	41.0
25	38.002145460884535	40.0	35.0	41.0	34.0	41.0
26	37.90785425668984	40.0	35.0	41.0	34.0	41.0
27	37.77923957386842	40.0	35.0	41.0	34.0	41.0
28	37.800857794801225	40.0	35.0	41.0	34.0	41.0
29	37.77352775903056	40.0	35.0	41.0	34.0	41.0
30	37.687172714957555	40.0	35.0	41.0	34.0	41.0
31	37.58832958875907	40.0	35.0	41.0	34.0	41.0
32	37.54689482761811	40.0	35.0	41.0	34.0	41.0
33	37.41754389040327	40.0	35.0	41.0	33.0	41.0
34	37.36756331933866	40.0	35.0	41.0	33.0	41.0
35	37.337182112913766	40.0	35.0	41.0	33.0	41.0
36	37.23674182109992	40.0	35.0	41.0	33.0	41.0
37	37.14892790258069	40.0	35.0	41.0	33.0	41.0
38	37.19989306781429	40.0	35.0	41.0	33.0	41.0
39	37.1816269469108	40.0	35.0	41.0	33.0	41.0
40	37.13793472851593	40.0	35.0	41.0	33.0	41.0
41	36.984112097653046	40.0	35.0	41.0	33.0	41.0
42	36.99008686048856	39.0	35.0	41.0	33.0	41.0
43	36.92364379700804	39.0	35.0	41.0	33.0	41.0
44	36.898814688859474	39.0	35.0	41.0	33.0	41.0
45	36.83032647428612	39.0	35.0	41.0	32.0	41.0
46	36.741835708143306	39.0	35.0	41.0	32.0	41.0
47	36.629801357396	39.0	35.0	41.0	32.0	41.0
48	36.54538044192793	39.0	35.0	41.0	32.0	41.0
49	36.56171632975237	39.0	35.0	41.0	32.0	41.0
50	36.43889137228665	39.0	35.0	41.0	31.0	41.0
51	36.37846592242644	38.0	35.0	41.0	31.0	41.0
52	36.1151760923785	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	1.0
14	4.0
15	9.0
16	44.0
17	70.0
18	166.0
19	319.0
20	514.0
21	809.0
22	1327.0
23	2138.0
24	3356.0
25	5222.0
26	6669.0
27	7831.0
28	9459.0
29	11362.0
30	14161.0
31	17538.0
32	22521.0
33	31065.0
34	69892.0
35	119426.0
36	75672.0
37	76072.0
38	112209.0
39	435081.0
40	3877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.15536818076019	25.053587828039802	27.00719406243944	12.78384992876057
2	33.149561899419474	25.066637839775076	27.645086427111305	14.138713833694155
3	30.941480436182033	23.81451843021993	31.204233657538477	14.03976747605956
4	26.968628356117293	27.33500256617768	30.764331396283083	14.932037681421944
5	22.48828664058612	31.050652549280837	29.710786419028086	16.750274391104956
6	24.068214553879503	37.28719472467884	26.81923493819261	11.825355783249043
7	83.28799915077536	1.1289234032482842	10.854006402296802	4.729071043679558
8	77.92999545197353	1.6372895320402134	12.164071759482441	8.268643256503823
9	72.27797693653896	3.1516752222154047	16.184741419860753	8.38560642138488
10	44.86730377992616	22.365285410573822	19.863578683292772	12.903832126207249
11	27.725723813057606	21.883993186725217	31.401834208365837	18.98844879185134
12	28.07145173589503	17.941623596758532	34.33594430956186	19.650980357784576
13	24.41063127970947	24.443938026078598	35.94226441076762	15.203166283444308
14	13.25218952902118	29.653424800281257	36.58434446577245	20.510041204925113
15	10.258477881690931	31.359372976152567	40.66646604708327	17.71568309507323
16	18.879958395783483	29.434885797789097	35.473243093476064	16.211912712951356
17	16.638764962471477	29.77749729991362	26.937756313430118	26.64598142418479
18	16.103324928736225	27.836259360218307	36.24884229839923	19.81157341264624
19	16.673629919197055	32.227880473579084	28.19776416291479	22.900725444309074
20	13.800776962638986	39.10718442101286	26.529018259303733	20.563020357044426
21	16.05404652621348	34.1383437587345	32.409119815663715	17.398489899388306
22	14.381794649300412	28.2088664117045	31.168200043045562	26.24113889594953
23	15.55317928476197	29.832716379420326	30.714371276729395	23.89973305908831
24	18.03229196187449	30.4545396997913	31.02036483547733	20.492803502856884
25	18.97676221417796	28.898764047022894	28.456914022821937	23.66755971597721
26	16.187760452426378	33.004745724416864	25.9168363655133	24.89065745764346
27	21.57468843096982	30.329493318686158	28.98923763584429	19.106580614499734
28	15.303281298846242	32.265667074723005	27.29244394581713	25.138607680613624
29	20.191874127767406	28.717914257527372	27.382722758343974	23.70748885636125
30	24.53645676599284	30.983162563217082	24.9568813977926	19.52349927299748
31	20.39619446075696	31.094087662966892	29.015629823756672	19.49408805251948
32	26.132745888028953	27.53893334657812	26.425786823188897	19.90253394220403
33	22.823009702781114	30.90155129579799	27.315817101163887	18.959621900257005
34	16.65756087489616	26.567291801184044	35.548134578732956	21.227012745186833
35	20.974095726705485	28.970052170830495	30.093619225978486	19.962232876485533
36	23.249667176006678	30.2535305638092	25.887132980593464	20.60966927959066
37	16.237428407538232	29.70737783387335	34.85064066792687	19.20455309066155
38	20.362303385504163	27.082669876580002	32.47066912474351	20.084357613172333
39	18.137081608345778	29.009494370478144	28.29193850133276	24.56148551984332
40	25.065566570155013	24.464973865890677	31.762754682178652	18.70670488177566
41	19.90565036291693	25.221484993947325	26.71356879839582	28.15929584473992
42	20.057283708229008	25.281183928228828	30.17581482228124	24.485717541260925
43	19.61445980255527	25.46700051323554	32.9756266683807	21.9429130158285
44	21.147057076271476	26.552488802797768	28.253664959452447	24.046789161478312
45	18.83106954584985	26.498827933647505	27.315524936722053	27.354577583780586
46	23.712066099283323	25.173277861044646	28.77556804071604	22.339087998956
47	18.104553967154875	24.52321197796301	32.79828285218719	24.573951202694925
48	16.440190530171332	27.060367990853308	30.709501869365486	25.789939609609874
49	24.39115365025384	21.718530724499644	31.204233657538477	22.686081967708038
50	19.532848535136182	22.46939334001416	32.43035043177035	25.567407693079307
51	17.4470865848801	23.372181465282587	27.617038640695196	31.563693309142117
52	18.04855578246994	24.117200791960414	34.27040208644367	23.56384133912598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	925.0
1	1462.0
2	1999.0
3	9354.5
4	16710.0
5	9195.0
6	1680.0
7	2300.0
8	2920.0
9	4678.0
10	6436.0
11	8581.0
12	10726.0
13	11445.5
14	11024.5
15	9884.0
16	8555.5
17	7227.0
18	6561.0
19	5895.0
20	5103.5
21	4312.0
22	4261.0
23	4210.0
24	3990.5
25	3771.0
26	5111.0
27	6451.0
28	6612.0
29	6773.0
30	8565.5
31	10358.0
32	10671.0
33	10984.0
34	14497.5
35	18011.0
36	20050.5
37	22090.0
38	22383.5
39	30642.0
40	38607.0
41	50651.0
42	62695.0
43	80896.0
44	99097.0
45	125036.0
46	150975.0
47	147565.5
48	144156.0
49	128741.0
50	113326.0
51	88947.5
52	64569.0
53	54878.5
54	45188.0
55	39779.0
56	34370.0
57	31373.5
58	28377.0
59	30642.5
60	32908.0
61	21535.0
62	10162.0
63	8602.0
64	5601.0
65	4160.0
66	2926.5
67	1693.0
68	1461.5
69	1230.0
70	974.5
71	719.0
72	668.0
73	617.0
74	375.5
75	134.0
76	129.5
77	125.0
78	171.0
79	217.0
80	172.5
81	128.0
82	77.5
83	27.0
84	38.5
85	50.0
86	44.0
87	38.0
88	20.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1026819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.001626922216296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.483044390712	12.497625428673013
2	12.930321106893338	5.172549175290789
3	6.613786373221717	3.968594627412564
4	3.9601937404459475	3.168412709443845
5	2.6975288498419303	2.6977482833226762
6	1.915148756681528	2.2983654559014197
7	1.3368548399513147	1.8717490230505764
8	0.9998028125631233	1.5998146282136114
9	0.7413959786586273	1.334621319008517
>10	4.842709464114958	19.437446123823733
>50	0.6930922214828086	9.834137120186268
>100	0.7559234909241771	25.972468077605882
>500	0.020456692409030565	2.6913455055005047
>1k	0.006818897469676855	2.3599201305018545
>5k	0.0019482564199076726	2.416619004178872
>10k+	9.741282099538363E-4	2.6785833878859187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15460	1.5056207569201583	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	12035	1.1720663524925035	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6432	0.6264005632930438	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	6307	0.6142270448832754	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	6203	0.6040986775663482	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	5864	0.5710840956390562	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	2685	0.2614871754418257	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2663	0.25934463620170645	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2224	0.21659123954659976	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	2123	0.20675503667150685	No Hit
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	1971	0.19195203828522847	Illumina Single End Adapter 2 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1860	0.1811419539373541	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1601	0.1559184237923139	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1583	0.1541654371413073	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGATTATGCTCG	1501	0.1461796090644992	No Hit
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1378	0.13420086694928707	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1265	0.12319600630685641	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1255	0.12222212483407494	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1060	0.10323143611483619	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGATTATGCT	1055	0.10274449537844549	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.634386391369852	0.0
2	0.0	0.0	0.0	2.3102416297322117	0.0
3	0.0	0.0	0.0	3.571320748836942	0.0
4	0.0	0.0	0.0	4.998836211640026	0.0
5	0.0	0.0	0.0	8.831936300360628	0.0
6	0.0	0.0	0.0	11.91758235872145	0.0
7	0.0	0.0	0.0	14.293853152308245	0.0
8	0.0	0.0	0.0	18.95913495952062	0.0
9	0.0	0.0	0.0	21.181727256702494	0.0
10	0.0	0.0	0.0	23.640875363622996	0.0
11	0.0	0.0	0.0	27.614896101455077	0.0
12	0.0	0.0	0.0	29.72412859520519	0.0
13	0.0	0.0	0.0	30.56488047065744	0.0
14	0.0	0.0	0.0	30.889962106271895	0.0
15	0.0	0.0	0.0	31.409333095706256	0.0
16	0.0	0.0	0.0	32.48927026087363	0.0
17	0.0	0.0	0.0	33.7902785203624	0.0
18	0.0	0.0	0.0	35.07560728813939	0.0
19	0.0	0.0	0.0	35.79881166982691	0.0
20	0.0	0.0	0.0	36.57850117693576	0.0
21	0.0	0.0	0.0	37.39422429853752	0.0
22	9.738814727814737E-5	0.0	0.0	38.255817237507294	0.0
23	9.738814727814737E-5	0.0	0.0	39.09617956037042	0.0
24	2.921644418344421E-4	0.0	0.0	39.64837035543752	0.0
25	2.921644418344421E-4	0.0	0.0	40.23445222575741	0.0
26	2.921644418344421E-4	0.0	0.0	40.74651910414591	0.0
27	2.921644418344421E-4	0.0	0.0	41.36892675340055	0.0
28	3.895525891125895E-4	0.0	0.0	41.85294584537294	0.0
29	3.895525891125895E-4	0.0	0.0	42.448279589684255	0.0
30	3.895525891125895E-4	0.0	0.0	43.61567131110741	0.0
31	3.895525891125895E-4	0.0	0.0	44.18471025565363	0.0
32	3.895525891125895E-4	0.0	0.0	44.58848151426882	0.0
33	3.895525891125895E-4	0.0	0.0	45.05974275894778	0.0
34	3.895525891125895E-4	0.0	0.0	45.571322696599886	0.0
35	3.895525891125895E-4	0.0	0.0	46.184965412599496	0.0
36	3.895525891125895E-4	0.0	0.0	46.62467289756033	0.0
37	3.895525891125895E-4	0.0	0.0	47.10469907549432	0.0
38	3.895525891125895E-4	0.0	0.0	47.62426484122324	0.0
39	3.895525891125895E-4	0.0	0.0	48.985653752024454	0.0
40	3.895525891125895E-4	0.0	0.0	49.43802169613145	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	6.3111365E-4	46.000004	20
TCACGAT	20	6.3111365E-4	46.000004	13
CGGGTGG	40	5.6097633E-9	46.000004	6
AACGTCA	20	6.3111365E-4	46.000004	35
CTATGCG	20	6.3111365E-4	46.000004	1
CAGCGCG	20	6.3111365E-4	46.000004	1
TCGTTGA	155	0.0	46.000004	24
GGTACCG	20	6.3111365E-4	46.000004	36
TCTTGAC	20	6.3111365E-4	46.000004	32
CGAACGT	20	6.3111365E-4	46.000004	33
ACGCCTA	20	6.3111365E-4	46.000004	30
TCCGCTC	40	5.6097633E-9	46.000004	38
CTACTTA	20	6.3111365E-4	46.000004	31
GCGACGT	20	6.3111365E-4	46.000004	32
ATGATGT	20	6.3111365E-4	46.000004	29
ACGCCCT	20	6.3111365E-4	46.000004	21
CTACTGA	20	6.3111365E-4	46.000004	19
TTAATCG	155	0.0	46.000004	20
CCGATAC	20	6.3111365E-4	46.000004	44
TCCGAAC	20	6.3111365E-4	46.000004	31
>>END_MODULE