FastQCFastQC Report
Sat 14 Jan 2017
SRR2933094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258631
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC246661.9597483297328604No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150571.1962997892154252No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG120820.9599318624759758No Hit
GGGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT33870.26910190516521526Illumina PCR Primer Index 7 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA29610.23525560708420495No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT26930.21396263082666803TruSeq Adapter, Index 23 (96% over 27bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCG23490.18663134786923252No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT21140.16796026794191468Illumina PCR Primer Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT20280.16112744720255578Illumina PCR Primer Index 7 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA20060.15937951631574304No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCAAGTCTTCT17780.14126459621604742No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG17520.13919885971345058No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15320.12171955084532321No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG15060.11965381434272633No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC13740.10916622902184994TruSeq Adapter, Index 23 (96% over 25bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.10503475601665618No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG12690.10082383160751641No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT351.0196709E-746.00000413
ATTAGCG750.046.01
CTATCGA206.311684E-446.031
CTATACG253.417343E-546.01
TAAGTCG301.8615447E-646.031
TACGATA206.311684E-446.044
CGTAATC206.311684E-446.034
CGTCGTA206.311684E-446.031
CGTAAGT206.311684E-446.030
GTATACG206.311684E-446.01
CTCGAAC501.6370905E-1146.013
CAATGCG206.311684E-446.01
ATAACGG206.311684E-446.02
ATACGAC301.8615447E-646.020
TATACGA301.8615447E-646.019
TGTACGT253.417343E-546.014
TTATTCG206.311684E-446.041
ATCGCGA206.311684E-446.027
TACGTTC206.311684E-446.037
TACCGTC206.311684E-446.014
Produced by FastQC (version 0.11.5)