##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1258631
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4591027870758	33.0	31.0	34.0	31.0	34.0
2	32.84569504485429	34.0	31.0	34.0	31.0	34.0
3	32.891387547263655	34.0	31.0	34.0	31.0	34.0
4	36.3384447069872	37.0	37.0	37.0	35.0	37.0
5	36.188704234998184	37.0	37.0	37.0	35.0	37.0
6	36.278658320031845	37.0	37.0	37.0	35.0	37.0
7	36.49486386399191	37.0	37.0	37.0	35.0	37.0
8	36.44515588762711	37.0	37.0	37.0	35.0	37.0
9	38.28349691053216	39.0	39.0	39.0	37.0	39.0
10	37.99371301040575	39.0	38.0	39.0	35.0	39.0
11	37.68816039013817	39.0	37.0	39.0	35.0	39.0
12	37.24546431797723	39.0	35.0	39.0	35.0	39.0
13	37.10860609662403	39.0	35.0	39.0	34.0	39.0
14	37.82285832781808	40.0	36.0	41.0	32.0	41.0
15	38.160170852299046	40.0	36.0	41.0	33.0	41.0
16	38.41085592202957	40.0	36.0	41.0	34.0	41.0
17	38.454164882320555	40.0	36.0	41.0	35.0	41.0
18	38.404709561420304	40.0	36.0	41.0	35.0	41.0
19	38.349996146606905	40.0	36.0	41.0	35.0	41.0
20	38.162399464179735	40.0	35.0	41.0	34.0	41.0
21	38.13288326761378	40.0	35.0	41.0	34.0	41.0
22	38.06876995719953	40.0	35.0	41.0	34.0	41.0
23	38.048476479603636	40.0	35.0	41.0	34.0	41.0
24	38.01006808190804	40.0	35.0	41.0	34.0	41.0
25	37.83733119556089	40.0	35.0	41.0	34.0	41.0
26	37.734163547536966	40.0	35.0	41.0	34.0	41.0
27	37.645557752828275	40.0	35.0	41.0	34.0	41.0
28	37.60914994148404	40.0	35.0	41.0	34.0	41.0
29	37.55013979474524	40.0	35.0	41.0	34.0	41.0
30	37.498417725290416	40.0	35.0	41.0	34.0	41.0
31	37.361426820092625	40.0	35.0	41.0	33.0	41.0
32	37.234840870755605	40.0	35.0	41.0	33.0	41.0
33	37.117967855550994	39.0	35.0	41.0	33.0	41.0
34	36.957696099968935	39.0	35.0	41.0	33.0	41.0
35	36.85531740438619	39.0	35.0	41.0	32.0	41.0
36	36.78559005776911	39.0	35.0	41.0	32.0	41.0
37	36.68025974252978	39.0	35.0	41.0	32.0	41.0
38	36.657645489424624	39.0	35.0	41.0	32.0	41.0
39	36.641192692695476	39.0	35.0	41.0	32.0	41.0
40	36.57575095480725	39.0	35.0	41.0	32.0	41.0
41	36.41046819917831	39.0	35.0	41.0	31.0	41.0
42	36.44550467929044	39.0	35.0	41.0	31.0	41.0
43	36.40844298289173	39.0	35.0	41.0	32.0	41.0
44	36.369005689515035	38.0	35.0	41.0	31.0	41.0
45	36.28763156159351	38.0	35.0	41.0	31.0	41.0
46	36.243356472230545	38.0	35.0	41.0	31.0	41.0
47	36.13424585919145	38.0	35.0	41.0	31.0	41.0
48	36.032719677173056	38.0	35.0	41.0	31.0	41.0
49	36.00515798514417	38.0	35.0	41.0	31.0	41.0
50	35.89195324125975	37.0	35.0	41.0	31.0	41.0
51	35.81860370513677	37.0	35.0	40.0	31.0	41.0
52	35.56143937341445	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	2.0
13	3.0
14	9.0
15	25.0
16	84.0
17	155.0
18	299.0
19	559.0
20	931.0
21	1450.0
22	2318.0
23	3590.0
24	5865.0
25	8907.0
26	11413.0
27	12757.0
28	13644.0
29	14782.0
30	17191.0
31	21079.0
32	27165.0
33	37718.0
34	101681.0
35	140438.0
36	80047.0
37	96278.0
38	150852.0
39	506429.0
40	2957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.41171002462199	22.32830750235772	25.0447510032726	13.215231469747687
2	39.090726352680015	22.41800813741279	24.965220147922622	13.526045361984568
3	34.27692469039774	23.2561409976395	28.896952323596036	13.569981988366726
4	30.921294644737017	26.50077743198761	28.36740871629572	14.210519206979647
5	26.0633974532647	29.691466363056367	28.834741874306292	15.410394309372643
6	27.048833216407353	37.321105232590014	26.32669940594185	9.303362145060785
7	85.98143538495397	1.9956603643164676	9.360328801690091	2.662575449039472
8	83.80915454966546	2.327052170175373	9.996734547297818	3.8670587328613393
9	77.78475184545749	4.512760292730753	12.469897849329946	5.232590012481816
10	50.30997965249545	18.98745541783096	17.826670406179414	12.875894523494177
11	33.80275871164781	24.459670864614015	25.415391802680848	16.322178621057322
12	32.71189093546877	17.803391144823223	31.396731845950082	18.087986073757918
13	29.62409157251013	19.897730152840666	34.16164070327204	16.316537571377154
14	18.883374078661657	21.051285086733127	39.668576413579515	20.3967644210257
15	16.73103554576361	28.100134193421265	37.60156868851951	17.567261572295614
16	29.942771153737674	23.45683524400718	29.971770916177974	16.628622686077176
17	26.546938697680254	23.341710159689377	26.724115328479915	23.387235814150454
18	23.510385490266806	23.570291848842114	33.49377220170169	19.425550459189388
19	24.070835693702126	25.94342583330619	26.603428645885884	23.3823098271058
20	19.13205697301274	36.489249033275044	26.001504809590735	18.377189184121477
21	24.706764730886178	26.325269280670827	32.50023239535654	16.467733593086457
22	19.930702485478268	22.865637347244743	35.85292273907126	21.350737428205726
23	22.09376695790903	23.629165339166125	34.17196938578503	20.105098317139813
24	24.102536803876593	27.425353419707605	28.84078018100619	19.631329595409618
25	24.846440299023303	24.77334500739295	30.039463512339992	20.34075118124375
26	19.038065962144586	29.19171703223582	26.267190304386272	25.503026701233324
27	23.637348833772567	25.570083686163773	30.572582432817878	20.21998504724578
28	18.39490684720144	29.01104453966254	26.856958075877678	25.73709053725834
29	22.41085751105765	29.75375626375006	25.866437422882484	21.96894880230981
30	25.588754766091093	25.67734308149092	25.65652681365706	23.077375338760923
31	23.80117762870929	26.31668852904465	29.722690764807158	20.159443077438898
32	26.975261216353324	25.15614187160494	25.17973893857691	22.68885797346482
33	25.287475042327735	25.15550626037337	28.14129002066531	21.41572867663358
34	19.883111094514597	23.868393516447632	34.21217179618172	22.036323592856046
35	21.490889704766527	29.393682501066635	28.695304660380998	20.420123133785836
36	24.460465378653474	26.23135772120661	25.822739150712167	23.485437749427753
37	20.510300477264582	27.996370659867747	28.463544915070422	23.02978394779725
38	22.471002223844796	24.485889827916203	31.513604861154697	21.5295030870843
39	20.158171854975762	26.581817864012564	26.767734149246287	26.492276131765387
40	26.01286636035502	23.165725299948914	29.841629516514374	20.979778823181693
41	19.333942990439613	24.410093188551688	27.67268564019161	28.583278180817096
42	18.601957205884805	26.65221180790875	30.774706804456585	23.971124181749854
43	19.37668784576258	26.557664637212973	31.02553488671422	23.040112630310233
44	24.54547838087573	24.346690968202754	27.040570270396962	24.067260380524555
45	19.37962754770858	25.180215647000594	25.991652835501426	29.448503969789396
46	23.26591352032486	23.64648574522636	28.163536413770203	24.92406432067858
47	18.073446466835794	24.095068371905665	32.50714466749985	25.324340493758697
48	19.082558748354366	28.565083809313453	29.140709230902466	23.21164821142972
49	24.191999084719825	24.77199433352587	28.85381021125334	22.182196370500964
50	21.27668871972802	24.625485944649387	28.793903852678028	25.303921482944563
51	20.233332883108712	25.67130477479102	27.057970127861143	27.03739221423912
52	20.41503824393329	26.301592762294906	29.039488142275218	24.243880851496584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	760.0
1	2721.5
2	4683.0
3	11885.5
4	19088.0
5	11530.5
6	3973.0
7	4314.0
8	4655.0
9	5953.0
10	7251.0
11	8832.5
12	10414.0
13	10716.0
14	10330.5
15	9643.0
16	8705.0
17	7767.0
18	7158.0
19	6549.0
20	6112.5
21	5676.0
22	5706.0
23	5736.0
24	6183.5
25	6631.0
26	7489.5
27	8348.0
28	8924.0
29	9500.0
30	11414.0
31	13328.0
32	13843.0
33	14358.0
34	17461.0
35	20564.0
36	22520.5
37	24477.0
38	26814.0
39	32816.5
40	36482.0
41	44224.5
42	51967.0
43	61012.5
44	70058.0
45	82199.5
46	94341.0
47	101685.0
48	109029.0
49	115721.0
50	122413.0
51	105654.5
52	88896.0
53	83424.5
54	77953.0
55	75358.5
56	72764.0
57	71737.5
58	70711.0
59	77375.0
60	84039.0
61	64136.5
62	44234.0
63	38369.0
64	28597.5
65	24691.0
66	20964.5
67	17238.0
68	15428.0
69	13618.0
70	11303.5
71	8989.0
72	7124.5
73	5260.0
74	4918.0
75	4576.0
76	3551.5
77	2527.0
78	1984.0
79	1441.0
80	1047.5
81	654.0
82	492.5
83	331.0
84	242.5
85	154.0
86	142.0
87	130.0
88	75.0
89	15.0
90	10.0
91	20.0
92	30.0
93	15.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1258631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.015983608471434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.07587601413333	15.648053212849073
2	11.26117661946204	4.958517597323561
3	4.535700441445779	2.9957371971542073
4	2.4107869537388087	2.1230338422812154
5	1.4400577908764158	1.5852144359593379
6	0.9097524413747563	1.2017456902244101
7	0.6712852148121191	1.0345303000138988
8	0.5344633606762673	0.9413389270381805
9	0.4521994086310315	0.8960053291363137
>10	4.658923864777002	24.552480874542184
>50	1.7049452978858934	26.774010482401977
>100	0.32924079253983535	9.409237447208666
>500	0.00870239980281418	1.3245386093027167
>1k	0.005801599868542786	2.4199652484572414
>5k	0.0	0.0
>10k+	0.0010877999753517724	4.135590806106994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	24666	1.9597483297328604	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15057	1.1962997892154252	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	12082	0.9599318624759758	No Hit
GGGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	3387	0.26910190516521526	Illumina PCR Primer Index 7 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2961	0.23525560708420495	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	2693	0.21396263082666803	TruSeq Adapter, Index 23 (96% over 27bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCG	2349	0.18663134786923252	No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2114	0.16796026794191468	Illumina PCR Primer Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2028	0.16112744720255578	Illumina PCR Primer Index 7 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2006	0.15937951631574304	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCAAGTCTTCT	1778	0.14126459621604742	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1752	0.13919885971345058	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1532	0.12171955084532321	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG	1506	0.11965381434272633	No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1374	0.10916622902184994	TruSeq Adapter, Index 23 (96% over 25bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1322	0.10503475601665618	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG	1269	0.10082383160751641	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21992148612262052	0.0
2	0.0	0.0	0.0	0.6635781257572712	0.0
3	0.0	0.0	0.0	1.0440708992548253	0.0
4	0.0	0.0	0.0	1.507113681452308	0.0
5	0.0	0.0	0.0	2.9670332289606725	0.0
6	0.0	0.0	0.0	4.25501993832982	0.0
7	0.0	0.0	0.0	5.298296323545185	0.0
8	0.0	0.0	0.0	7.120514273047462	0.0
9	0.0	0.0	0.0	8.12827588069895	0.0
10	0.0	0.0	0.0	9.219779268109557	0.0
11	0.0	0.0	0.0	10.817467550060343	0.0
12	0.0	0.0	0.0	11.74776403886445	0.0
13	0.0	0.0	0.0	12.19865075625819	0.0
14	0.0	0.0	0.0	12.552686212241714	0.0
15	0.0	0.0	0.0	12.856508380931345	0.0
16	0.0	0.0	0.0	13.329244234410243	0.0
17	0.0	0.0	0.0	13.84250030390162	0.0
18	0.0	0.0	0.0	14.488042960963142	0.0
19	0.0	0.0	0.0	14.861385108105553	0.0
20	7.945140394603342E-5	0.0	0.0	15.397523181933387	0.0
21	7.945140394603342E-5	0.0	0.0	15.867637139082067	0.0
22	7.945140394603342E-5	0.0	0.0	16.36087145477904	0.0
23	7.945140394603342E-5	0.0	0.0	16.813426651655647	0.0
24	3.9725701973016713E-4	0.0	0.0	17.151412924042074	0.0
25	3.9725701973016713E-4	0.0	0.0	17.54668365867359	0.0
26	3.9725701973016713E-4	0.0	0.0	17.869653615714217	0.0
27	3.9725701973016713E-4	0.0	0.0	18.36598653616509	0.0
28	3.9725701973016713E-4	0.0	0.0	18.738375266460146	0.0
29	3.9725701973016713E-4	0.0	0.0	19.219771322969162	0.0
30	3.9725701973016713E-4	0.0	0.0	20.53659889197072	0.0
31	3.9725701973016713E-4	0.0	0.0	21.037698896658352	0.0
32	3.9725701973016713E-4	0.0	0.0	21.376956391507917	0.0
33	3.9725701973016713E-4	0.0	0.0	21.79089820606675	0.0
34	3.9725701973016713E-4	0.0	0.0	22.24758487594855	0.0
35	3.9725701973016713E-4	0.0	0.0	22.66486364947312	0.0
36	3.9725701973016713E-4	0.0	0.0	22.969321429394316	0.0
37	3.9725701973016713E-4	0.0	0.0	23.346795049541925	0.0
38	3.9725701973016713E-4	0.0	0.0	23.841538941913875	0.0
39	3.9725701973016713E-4	0.0	0.0	25.48387891288233	0.0
40	3.9725701973016713E-4	0.0	0.0	25.88169209244012	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTT	35	1.0196709E-7	46.000004	13
ATTAGCG	75	0.0	46.0	1
CTATCGA	20	6.311684E-4	46.0	31
CTATACG	25	3.417343E-5	46.0	1
TAAGTCG	30	1.8615447E-6	46.0	31
TACGATA	20	6.311684E-4	46.0	44
CGTAATC	20	6.311684E-4	46.0	34
CGTCGTA	20	6.311684E-4	46.0	31
CGTAAGT	20	6.311684E-4	46.0	30
GTATACG	20	6.311684E-4	46.0	1
CTCGAAC	50	1.6370905E-11	46.0	13
CAATGCG	20	6.311684E-4	46.0	1
ATAACGG	20	6.311684E-4	46.0	2
ATACGAC	30	1.8615447E-6	46.0	20
TATACGA	30	1.8615447E-6	46.0	19
TGTACGT	25	3.417343E-5	46.0	14
TTATTCG	20	6.311684E-4	46.0	41
ATCGCGA	20	6.311684E-4	46.0	27
TACGTTC	20	6.311684E-4	46.0	37
TACCGTC	20	6.311684E-4	46.0	14
>>END_MODULE