FastQCFastQC Report
Sat 14 Jan 2017
SRR2933093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694250
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT515183.040755496532389No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC103340.6099454035709015No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG51050.30131326545669174No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC50760.299601593625498No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT39800.2349122030396931No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT39050.23048546554522653No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC29600.1747085731149476No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT21320.1258373911760366Illumina Single End Adapter 1 (95% over 21bp)
GGGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT19760.11662977718754611No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT19660.11603954552161723No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG18810.11102257636122177No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA17910.10571049136786188No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT17910.10571049136786188No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA206.3123053E-446.00000435
GCTCGAT206.3123053E-446.0000049
CGAAGTA206.3123053E-446.00000423
GTCACGA253.4178465E-546.038
TCCGTAA253.4178465E-546.022
TATAGCG900.046.01
TTACGCG253.4178465E-546.01
TATCGCT253.4178465E-546.046
TCGCGCG253.4178465E-546.01
CGTTTTT123750.044.5503041
TATTGCG1200.044.0833321
TGCGTAG750.042.933331
ATACGAA12100.042.3884319
CGGGATA2450.042.24496
CGAATAT2300.042.00000414
CATACGA12250.041.49387718
AGGGCGA3900.041.282056
TCTCGCG950.041.15789432
ACATACG12350.041.15789417
GTACGAG950.041.1578941
Produced by FastQC (version 0.11.5)