##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1694250
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52140002951158	33.0	31.0	34.0	31.0	34.0
2	32.90767360188874	34.0	31.0	34.0	31.0	34.0
3	32.91078471299985	34.0	31.0	34.0	31.0	34.0
4	36.38310756972112	37.0	37.0	37.0	35.0	37.0
5	36.320553637302645	37.0	37.0	37.0	35.0	37.0
6	36.398397521027	37.0	37.0	37.0	35.0	37.0
7	36.55132831636417	37.0	37.0	37.0	35.0	37.0
8	36.50067463479416	37.0	37.0	37.0	35.0	37.0
9	38.340935517190495	39.0	39.0	39.0	37.0	39.0
10	38.03302641286705	39.0	39.0	39.0	35.0	39.0
11	37.76804013575328	39.0	38.0	39.0	35.0	39.0
12	37.486958831341305	39.0	37.0	39.0	35.0	39.0
13	37.41934661354582	39.0	37.0	39.0	35.0	39.0
14	38.56188460970931	41.0	38.0	41.0	34.0	41.0
15	38.73055629334514	41.0	38.0	41.0	34.0	41.0
16	38.880293935369636	41.0	38.0	41.0	35.0	41.0
17	38.895045005164526	41.0	38.0	41.0	35.0	41.0
18	38.74969632580788	40.0	38.0	41.0	35.0	41.0
19	38.64319551423934	40.0	37.0	41.0	35.0	41.0
20	38.45800147557917	40.0	35.0	41.0	35.0	41.0
21	38.413782499631104	40.0	35.0	41.0	35.0	41.0
22	38.35357591854803	40.0	35.0	41.0	35.0	41.0
23	38.304594953519256	40.0	35.0	41.0	35.0	41.0
24	38.251530765825585	40.0	35.0	41.0	35.0	41.0
25	38.143695440460384	40.0	35.0	41.0	35.0	41.0
26	38.03344901873986	40.0	35.0	41.0	34.0	41.0
27	37.92790910432345	40.0	35.0	41.0	34.0	41.0
28	37.879202006787665	40.0	35.0	41.0	34.0	41.0
29	37.80806020362993	40.0	35.0	41.0	34.0	41.0
30	37.75174324922532	40.0	35.0	41.0	34.0	41.0
31	37.626538586395164	40.0	35.0	41.0	34.0	41.0
32	37.47736402537996	40.0	35.0	41.0	34.0	41.0
33	37.291367271654124	40.0	35.0	41.0	33.0	41.0
34	37.08144429688653	40.0	35.0	41.0	33.0	41.0
35	36.973079533716984	40.0	35.0	41.0	33.0	41.0
36	36.88276641581821	40.0	35.0	41.0	33.0	41.0
37	36.78841729378781	40.0	35.0	41.0	32.0	41.0
38	36.75247277556441	40.0	35.0	41.0	32.0	41.0
39	36.68736491072746	40.0	35.0	41.0	32.0	41.0
40	36.64326575180758	40.0	35.0	41.0	32.0	41.0
41	36.54284609709311	40.0	35.0	41.0	31.0	41.0
42	36.52711583296444	39.0	35.0	41.0	31.0	41.0
43	36.47248635089272	39.0	35.0	41.0	31.0	41.0
44	36.408142836063156	39.0	35.0	41.0	31.0	41.0
45	36.356602626530915	39.0	35.0	41.0	31.0	41.0
46	36.28875136491073	39.0	35.0	41.0	31.0	41.0
47	36.220357680389554	39.0	35.0	41.0	31.0	41.0
48	36.126549210565145	39.0	35.0	41.0	31.0	41.0
49	36.07789582411096	39.0	35.0	41.0	31.0	41.0
50	35.98902346170872	38.0	35.0	41.0	31.0	41.0
51	35.912541242437655	38.0	35.0	41.0	31.0	41.0
52	35.69593979637008	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	5.0
13	8.0
14	11.0
15	33.0
16	63.0
17	151.0
18	323.0
19	638.0
20	1042.0
21	1829.0
22	2874.0
23	4702.0
24	7916.0
25	13391.0
26	18076.0
27	19912.0
28	19892.0
29	20123.0
30	21468.0
31	24777.0
32	30862.0
33	41650.0
34	102897.0
35	165164.0
36	125312.0
37	121777.0
38	188634.0
39	755016.0
40	5703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.42703261029954	22.21401800206581	25.824169986719784	17.53477940091486
2	38.112911317692195	22.737317397078353	25.953666814224587	13.196104471004869
3	28.898804780876493	23.67395602774089	33.80392504057843	13.623314150804191
4	25.565648517042938	26.863449904087354	33.32630957650878	14.244592002360926
5	24.664423786336137	31.197402980669914	30.08221927106389	14.055953961930056
6	26.19766858491958	38.40212483399734	25.94481333923565	9.455393241847425
7	84.49201711671832	1.601711671831194	11.580227239191384	2.3260439722591117
8	82.9701047661207	1.9149476169396489	12.223697801387043	2.8912498155526043
9	77.81342777039988	3.9226206285967242	14.076730116570754	4.18722148443264
10	42.092666371550834	22.029275490630074	20.48971521322119	15.388342924597906
11	27.296975062712114	24.56697653829128	32.535458167330674	15.600590231665928
12	28.846215139442233	20.148679356647484	32.27510697948945	18.729998524420836
13	25.949830308396045	21.687118193891102	35.043411539029066	17.319639958683783
14	18.147321823815847	23.45592445034676	36.77131474103585	21.625438984801534
15	14.545137966651911	29.66522059908514	38.131857754168514	17.65778368009444
16	23.42523240371846	28.15511288180611	32.66377453150361	15.755880182971815
17	22.25769514534455	28.449343367271656	30.556057252471597	18.736904234912203
18	18.672568983325956	26.934985981997933	35.35369632580788	19.03874870886823
19	19.206197432492253	29.158211598052237	31.192799173675667	20.442791795779844
20	16.957119669470266	33.90101814962373	30.98214549210565	18.159716688800355
21	21.183296443854214	29.714918105356354	33.56582558654272	15.535959864246717
22	19.788756086764057	25.898539176626826	34.35915596871772	19.953548767891398
23	18.5253061826767	26.526840785008115	35.48803305297329	19.45981997934189
24	18.91834144901874	27.6606167920909	34.25509812601446	19.165943632875905
25	20.57777777777778	28.74581673306773	31.775593920613844	18.90081156854065
26	18.128670503172494	31.02257636122178	29.361192267965176	21.48756086764055
27	19.910461856278587	29.02871477054744	31.873041168658702	19.187782204515273
28	17.46023314150804	30.42614726280065	29.88502287147706	22.228596724214253
29	18.681717574147854	29.595986424671683	29.829482071713148	21.892813929467316
30	20.691692489302053	29.30647779253357	29.15868378338498	20.8431459347794
31	19.378899217943044	29.423461708720673	29.939619300575476	21.258019772760807
32	20.68968570163789	30.546672568983325	27.45096650435296	21.312675225025824
33	20.36730116570754	29.54994835472923	28.548915449313856	21.533835030249374
34	17.610683193153314	30.33625498007968	31.00841080123949	21.044651025527518
35	17.90184447395603	32.458314888593776	27.930824848753133	21.709015788697066
36	19.20265604249668	31.445477349859818	27.93908809207614	21.41277851556736
37	17.50025084845802	32.105651468201266	29.92374206876199	20.47035561457872
38	18.977246569278442	29.55219123505976	30.70107717279032	20.76948502287148
39	17.896237273129703	31.15319462889184	28.8315183709606	22.119049727017853
40	21.49777187546112	28.81735281097831	30.0282130736314	19.656662239929172
41	17.91742658993655	28.79740298066991	29.750686144311643	23.534484285081895
42	17.58571639368452	29.46755201416556	31.89476169396488	21.05196989818504
43	18.609237125571788	30.03576803895529	30.965205843293493	20.38978899217943
44	19.994983030839606	29.70482514386897	28.87808764940239	21.422104175889036
45	18.616792090895675	28.958418179135307	28.55528995130589	23.869499778663126
46	20.103054448871184	28.88398996606168	28.72580787959274	22.2871477054744
47	18.104352958536225	28.458373911760365	31.161634941714624	22.275638187988786
48	17.58896266784713	29.64662830160838	31.06713885199941	21.69727017854508
49	20.151335399144163	28.41044710048694	30.120643352515863	21.317574147853033
50	18.167271654124242	28.128611480005905	31.597639073336286	22.106477792533568
51	17.53424819241552	28.32663420392504	30.96963258078796	23.169485022871477
52	18.26164969750627	28.957414785303232	30.412926073483842	22.368009443706654
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1514.0
1	3207.0
2	4900.0
3	32894.0
4	60888.0
5	34585.0
6	8282.0
7	9200.0
8	10118.0
9	12729.5
10	15341.0
11	18158.5
12	20976.0
13	21772.5
14	21275.5
15	19982.0
16	18157.5
17	16333.0
18	14956.5
19	13580.0
20	12862.0
21	12144.0
22	12341.0
23	12538.0
24	13020.5
25	13503.0
26	15108.5
27	16714.0
28	19231.0
29	21748.0
30	25377.5
31	29007.0
32	31737.5
33	34468.0
34	38149.0
35	41830.0
36	44523.5
37	47217.0
38	50968.5
39	60648.5
40	66577.0
41	74893.0
42	83209.0
43	95731.5
44	108254.0
45	121022.5
46	133791.0
47	136298.5
48	138806.0
49	137095.5
50	135385.0
51	122169.5
52	108954.0
53	101124.0
54	93294.0
55	86766.0
56	80238.0
57	75931.5
58	71625.0
59	70146.0
60	68667.0
61	55946.5
62	43226.0
63	36359.0
64	25344.5
65	21197.0
66	16606.0
67	12015.0
68	9679.0
69	7343.0
70	6044.0
71	4745.0
72	4373.5
73	4002.0
74	3205.0
75	2408.0
76	1937.5
77	1467.0
78	1088.5
79	710.0
80	461.0
81	212.0
82	155.0
83	98.0
84	83.5
85	69.0
86	71.0
87	73.0
88	44.0
89	9.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1694250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.728582397119176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.625055960194	16.995610420497353
2	10.418665077677817	4.944403055273323
3	4.395199671100509	3.128755726424986
4	2.4222359996412433	2.299049060110223
5	1.5458425825863582	1.834032654693795
6	1.0285017182200473	1.4642932659817824
7	0.7497498511719066	1.2453350784532298
8	0.5800597422844027	1.101119631203773
9	0.46917385701379144	1.0019547472253363
>10	5.131278500987841	28.366575124144017
>50	1.3337721934190963	22.239439741117064
>100	0.2931975561060988	8.784814819013356
>500	0.002255365736985608	0.40238560765314246
>1k	0.0040095390879744145	1.908938253655805
>5k	5.011923859968018E-4	0.6053920306659736
>10k+	5.011923859968018E-4	3.6779007838868285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51518	3.040755496532389	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	10334	0.6099454035709015	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5105	0.30131326545669174	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	5076	0.299601593625498	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTT	3980	0.2349122030396931	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	3905	0.23048546554522653	No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2960	0.1747085731149476	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	2132	0.1258373911760366	Illumina Single End Adapter 1 (95% over 21bp)
GGGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	1976	0.11662977718754611	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCT	1966	0.11603954552161723	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	1881	0.11102257636122177	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1791	0.10571049136786188	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1791	0.10571049136786188	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13038217500368895	0.0
2	0.0	0.0	0.0	0.3543750922236978	0.0
3	0.0	0.0	0.0	0.6608233731739708	0.0
4	0.0	0.0	0.0	0.9775416851114063	0.0
5	0.0	0.0	0.0	1.9906743396783237	0.0
6	0.0	0.0	0.0	3.4002065810830753	0.0
7	0.0	0.0	0.0	4.481451969898185	0.0
8	0.0	0.0	0.0	5.995573262505533	0.0
9	0.0	0.0	0.0	6.90996015936255	0.0
10	0.0	0.0	0.0	7.751748561310314	0.0
11	0.0	0.0	0.0	8.776095617529881	0.0
12	0.0	0.0	0.0	9.36821602478973	0.0
13	0.0	0.0	0.0	9.681274900398407	0.0
14	0.0	0.0	0.0	10.010151984653977	0.0
15	0.0	0.0	0.0	10.238689685701639	0.0
16	5.9023166592887706E-5	0.0	0.0	10.561310314298362	0.0
17	5.9023166592887706E-5	0.0	0.0	10.907835325365205	0.0
18	5.9023166592887706E-5	0.0	0.0	11.277438394569868	0.0
19	5.9023166592887706E-5	0.0	0.0	11.540976833407113	0.0
20	5.9023166592887706E-5	0.0	0.0	11.861000442673749	0.0
21	5.9023166592887706E-5	0.0	0.0	12.20357090157887	0.0
22	5.9023166592887706E-5	0.0	0.0	12.553401209974915	0.0
23	5.9023166592887706E-5	0.0	0.0	12.903113472037775	0.0
24	1.1804633318577541E-4	0.0	0.0	13.181053563523683	0.0
25	1.1804633318577541E-4	0.0	0.0	13.466253504500516	0.0
26	1.1804633318577541E-4	0.0	0.0	13.717633171019624	0.0
27	1.1804633318577541E-4	0.0	0.0	14.006492548325218	0.0
28	1.1804633318577541E-4	0.0	0.0	14.259760956175299	0.0
29	1.1804633318577541E-4	0.0	0.0	14.568097978456544	0.0
30	1.1804633318577541E-4	0.0	0.0	15.180463331857753	0.0
31	1.1804633318577541E-4	0.0	0.0	15.51990556293345	0.0
32	1.1804633318577541E-4	0.0	0.0	15.87310019182529	0.0
33	1.1804633318577541E-4	0.0	0.0	16.183384978604103	0.0
34	1.1804633318577541E-4	0.0	0.0	16.498863804043086	0.0
35	1.1804633318577541E-4	0.0	0.0	16.818474251143574	0.0
36	1.1804633318577541E-4	0.0	0.0	17.09842113029364	0.0
37	1.1804633318577541E-4	0.0	0.0	17.40091485908219	0.0
38	1.1804633318577541E-4	0.0	0.0	17.735635236830458	0.0
39	1.1804633318577541E-4	0.0	0.0	18.56532388962668	0.0
40	1.1804633318577541E-4	0.0	0.0	18.93451379666519	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTA	20	6.3123053E-4	46.000004	35
GCTCGAT	20	6.3123053E-4	46.000004	9
CGAAGTA	20	6.3123053E-4	46.000004	23
GTCACGA	25	3.4178465E-5	46.0	38
TCCGTAA	25	3.4178465E-5	46.0	22
TATAGCG	90	0.0	46.0	1
TTACGCG	25	3.4178465E-5	46.0	1
TATCGCT	25	3.4178465E-5	46.0	46
TCGCGCG	25	3.4178465E-5	46.0	1
CGTTTTT	12375	0.0	44.550304	1
TATTGCG	120	0.0	44.083332	1
TGCGTAG	75	0.0	42.93333	1
ATACGAA	1210	0.0	42.38843	19
CGGGATA	245	0.0	42.2449	6
CGAATAT	230	0.0	42.000004	14
CATACGA	1225	0.0	41.493877	18
AGGGCGA	390	0.0	41.28205	6
TCTCGCG	95	0.0	41.157894	32
ACATACG	1235	0.0	41.157894	17
GTACGAG	95	0.0	41.157894	1
>>END_MODULE