FastQCFastQC Report
Sat 14 Jan 2017
SRR2933092.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933092.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271062
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.31988998176328143No Hit
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT33060.26009746180752785RNA PCR Primer, Index 16 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT27300.21478102563053572RNA PCR Primer, Index 16 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23450.18449139381084478No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT21530.16938591508518075Illumina PCR Primer Index 7 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19890.15648331867367601No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC14040.11045881318141838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT1100.046.0000043
CTATTCG551.8189894E-1246.00000444
TGCGTTA551.8189894E-1246.00000421
GTGCAAC206.311709E-446.023
AACGTAC206.311709E-446.040
TCGCACT206.311709E-446.017
ACGCCCC206.311709E-446.018
CGAGTGT206.311709E-446.026
CGACGGA253.417361E-546.015
ACGTTAC301.8615592E-646.043
ACCGCGA206.311709E-446.026
ATAATCG206.311709E-446.034
CGGCACT301.8615592E-646.05
ATTACCG206.311709E-446.011
CCGGAAC301.8615592E-646.020
ACTTCGA253.417361E-546.014
GTGATCG206.311709E-446.015
TAAGTCG206.311709E-446.023
TAACGCG405.6115823E-946.01
ATCCGTC206.311709E-446.014
Produced by FastQC (version 0.11.5)