##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2933092.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1271062 Sequences flagged as poor quality 0 Sequence length 52 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.59441002877909 33.0 31.0 34.0 31.0 34.0 2 32.9566205267721 34.0 31.0 34.0 31.0 34.0 3 33.0394197922682 34.0 33.0 34.0 31.0 34.0 4 36.4328474928839 37.0 37.0 37.0 35.0 37.0 5 36.410947695706426 37.0 37.0 37.0 35.0 37.0 6 36.54082885020558 37.0 37.0 37.0 35.0 37.0 7 36.595188118282195 37.0 37.0 37.0 35.0 37.0 8 36.45219588029538 37.0 37.0 37.0 35.0 37.0 9 38.131632445938905 39.0 39.0 39.0 37.0 39.0 10 37.94201463028554 39.0 38.0 39.0 35.0 39.0 11 37.442655039644016 39.0 35.0 39.0 35.0 39.0 12 37.274861493774495 39.0 35.0 39.0 35.0 39.0 13 37.28411438623765 39.0 35.0 39.0 35.0 39.0 14 38.65642509964109 41.0 36.0 41.0 35.0 41.0 15 38.72225194365027 41.0 36.0 41.0 35.0 41.0 16 38.7043259888188 41.0 36.0 41.0 35.0 41.0 17 38.72120950826946 40.0 36.0 41.0 35.0 41.0 18 38.66726406737043 40.0 36.0 41.0 35.0 41.0 19 38.577740503610364 40.0 36.0 41.0 35.0 41.0 20 38.49050715071334 40.0 35.0 41.0 35.0 41.0 21 38.4263458430824 40.0 35.0 41.0 35.0 41.0 22 38.401734927171134 40.0 35.0 41.0 35.0 41.0 23 38.38099321669596 40.0 35.0 41.0 35.0 41.0 24 38.33937604931939 40.0 35.0 41.0 35.0 41.0 25 38.27611634994989 40.0 35.0 41.0 35.0 41.0 26 38.205732686525124 40.0 35.0 41.0 35.0 41.0 27 38.186962555721124 40.0 35.0 41.0 35.0 41.0 28 38.21640801156828 40.0 36.0 41.0 35.0 41.0 29 38.17933900942676 40.0 36.0 41.0 35.0 41.0 30 38.1110795539478 40.0 35.0 41.0 35.0 41.0 31 38.0194719061698 40.0 35.0 41.0 34.0 41.0 32 37.95248225499621 40.0 35.0 41.0 34.0 41.0 33 37.82198744042383 40.0 35.0 41.0 34.0 41.0 34 37.734491315136474 40.0 35.0 41.0 34.0 41.0 35 37.64613449225923 40.0 35.0 41.0 34.0 41.0 36 37.58145157356604 40.0 35.0 41.0 34.0 41.0 37 37.492791854370594 40.0 35.0 41.0 34.0 41.0 38 37.52509240304564 40.0 35.0 41.0 34.0 41.0 39 37.4500850469922 40.0 35.0 41.0 33.0 41.0 40 37.46150935202217 40.0 35.0 41.0 34.0 41.0 41 37.421878712446755 40.0 35.0 41.0 34.0 41.0 42 37.362357619061854 40.0 35.0 41.0 34.0 41.0 43 37.27683700716409 40.0 35.0 41.0 33.0 41.0 44 37.20167623609234 39.0 35.0 41.0 33.0 41.0 45 37.147673362904406 39.0 35.0 41.0 33.0 41.0 46 37.0900986733928 39.0 35.0 41.0 33.0 41.0 47 37.029966280165716 39.0 35.0 41.0 33.0 41.0 48 37.01336992215958 39.0 35.0 41.0 33.0 41.0 49 36.92179217064156 39.0 35.0 41.0 33.0 41.0 50 36.81017369727047 39.0 35.0 41.0 33.0 41.0 51 36.7233124741358 39.0 35.0 41.0 33.0 41.0 52 36.49656035661518 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 0.0 12 0.0 13 4.0 14 12.0 15 24.0 16 40.0 17 81.0 18 146.0 19 297.0 20 491.0 21 915.0 22 1414.0 23 2243.0 24 3496.0 25 5471.0 26 7606.0 27 8994.0 28 9803.0 29 10767.0 30 12637.0 31 15432.0 32 20136.0 33 27562.0 34 66781.0 35 191135.0 36 58397.0 37 76603.0 38 142078.0 39 603087.0 40 5407.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.676690830187674 22.814308035327937 24.64631937702488 19.862681757459512 2 25.89975941378155 24.271672034881068 26.77894548023621 23.049623071101173 3 27.1951328888756 25.52896711568751 25.827851041097915 21.44804895433897 4 24.170732820271553 29.2340578193668 24.130844915511595 22.464364444850055 5 22.324638766637662 37.542700513428926 21.509808333503795 18.622852386429617 6 89.40390004578849 2.9386450070885606 5.871232087813183 1.7862228593097742 7 91.37020853428078 2.127118897425932 4.098619894230179 2.4040526740631063 8 84.21249317499854 4.715033570352981 7.443381990807686 3.629091263840788 9 45.830887871716726 30.578130728477447 11.705251199390746 11.885730200415086 10 34.321929221391244 22.375777106073507 28.188396789456377 15.113896883078876 11 33.556427617220876 22.467668768321296 27.087427678586884 16.88847593587095 12 22.10576667385226 33.073130972367984 27.88376963515549 16.93733271862427 13 16.66000557014528 36.16526967213244 29.280082324859052 17.89464243286323 14 15.711979431373136 27.03518789799396 39.99852092187478 17.254311748758123 15 18.19683068174487 22.24462693401266 40.58244208386373 18.976100300378736 16 18.56998321088979 22.524314313542533 29.416346330863487 29.489356144704193 17 20.853349403884312 24.516270646121118 32.621068051755145 22.009311898239424 18 25.5797907576499 26.51955608774395 28.22765529926943 19.672997855336718 19 27.700301008133355 24.014800222176415 27.47702315071964 20.80787561897059 20 21.38086104375711 28.86877272705816 28.91794420728493 20.832422021899795 21 21.413510906627685 24.692501231253868 28.146384676750625 25.74760318536783 22 18.397686344175185 29.5151613375272 26.74582357115546 25.341328747142157 23 17.496471454578927 25.786940369549242 36.18344345122425 20.533144724647578 24 19.2676675095314 25.688912106569152 33.56303626416335 21.480384119736094 25 17.470272889914103 32.50754093820758 28.010041996377833 22.012144175500488 26 17.019626107931792 31.372663174573702 28.968767849247325 22.638942868247184 27 17.6701057855557 28.407976951557046 34.883270839660064 19.038646423227192 28 17.464923032865432 25.178394130262728 33.22756875746423 24.129114079407614 29 18.239629538134253 29.642141768064818 29.89460781614115 22.223620877659783 30 26.518926692797045 27.204101766868966 25.170841390899895 21.106130149434097 31 25.781983884342385 27.164921931424274 27.242494858630028 19.810599325603313 32 24.053822708884383 28.385869454047086 24.76684850935674 22.793459327711787 33 19.225576722457284 27.058396836660997 27.675833279572515 26.040193161309205 34 18.477619502431825 27.4023611751433 29.998930028590266 24.121089293834604 35 24.78502228844856 26.20186898829483 26.324994374782662 22.688114348473952 36 20.87333269344847 29.57566192679822 26.454177687634434 23.096827692118875 37 19.382138715499323 30.71565352437568 26.653853234539305 23.24835452558569 38 18.71600283857121 29.21604138901171 26.960525922417634 25.107429849999452 39 22.5777342096609 26.151753415647704 26.842357021136653 24.428155353554747 40 18.258668735278057 25.114825240625553 29.148224083482944 27.478281940613442 41 20.767909039842277 26.726941722748375 25.095235322903207 27.40991391450614 42 20.684356860640946 24.955666993427545 27.642868719228485 26.717107426703024 43 21.2073053871487 26.647087238860102 28.292797676273857 23.85280969771734 44 18.580918948092226 31.321288812032773 26.4288445410216 23.668947698853398 45 21.313358435701797 29.058771326654405 26.516409113009438 23.11146112463436 46 21.836621659683004 26.186212788990627 27.438787407695298 24.538378143631075 47 19.770396723369906 24.726645907123334 30.736030185781654 24.766927183725105 48 19.65380130945619 23.74416039500827 30.70180683554382 25.900231459991723 49 19.819568203596678 27.595270726368977 29.339796170446448 23.2453648995879 50 18.06080269884553 27.688578527247294 28.667366344049306 25.58325242985787 51 19.265621975953966 24.781167244398777 29.790128254955306 26.16308252469195 52 22.09443756480801 23.335525725731713 30.453825226464172 24.116211482996107 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2561.0 1 2003.0 2 1445.0 3 4075.0 4 6705.0 5 4513.0 6 2321.0 7 2412.0 8 2503.0 9 2751.0 10 2999.0 11 3211.5 12 3424.0 13 3345.5 14 3205.0 15 3143.0 16 3256.5 17 3370.0 18 3095.0 19 2820.0 20 3115.5 21 3411.0 22 4057.0 23 4703.0 24 4746.0 25 4789.0 26 7659.5 27 10530.0 28 11729.0 29 12928.0 30 14734.5 31 16541.0 32 18377.5 33 20214.0 34 25321.0 35 30428.0 36 32888.0 37 35348.0 38 38855.5 39 48754.0 40 55145.0 41 65286.0 42 75427.0 43 91329.0 44 107231.0 45 113185.5 46 119140.0 47 114257.0 48 109374.0 49 114260.5 50 119147.0 51 121460.5 52 123774.0 53 110807.5 54 97841.0 55 85351.0 56 72861.0 57 62303.5 58 51746.0 59 45250.5 60 38755.0 61 35797.5 62 32840.0 63 27627.0 64 17901.0 65 13388.0 66 10251.0 67 7114.0 68 5299.0 69 3484.0 70 2834.0 71 2184.0 72 1840.0 73 1496.0 74 1226.0 75 956.0 76 713.0 77 470.0 78 286.0 79 102.0 80 145.5 81 189.0 82 133.0 83 77.0 84 75.0 85 73.0 86 40.5 87 8.0 88 7.5 89 5.0 90 3.0 91 1.5 92 0.0 93 1.5 94 3.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1271062.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.38463011139192 #Duplication Level Percentage of deduplicated Percentage of total 1 70.69909625894435 12.997767339303842 2 12.168299185129124 4.474193592067014 3 5.014122329074058 2.765483530598926 4 2.4317565641090035 1.7882777980837337 5 1.4773002820648333 1.3579809624608459 6 0.9049201405754014 0.9981973238896522 7 0.6120676311261695 0.7876845900987349 8 0.4376967090220475 0.6437513677075106 9 0.33847773903465384 0.5600509229783118 >10 3.027752806670481 13.154595893018522 >50 1.3981094108927365 18.82353894184391 >100 1.4818156844668664 39.46149091093715 >500 0.005151155334183776 0.6706486019033775 >1k 0.003434103556122518 1.5163382251084367 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4066 0.31988998176328143 No Hit CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT 3306 0.26009746180752785 RNA PCR Primer, Index 16 (96% over 27bp) CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT 2730 0.21478102563053572 RNA PCR Primer, Index 16 (96% over 26bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2345 0.18449139381084478 No Hit GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT 2153 0.16938591508518075 Illumina PCR Primer Index 7 (95% over 23bp) GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 1989 0.15648331867367601 No Hit GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC 1404 0.11045881318141838 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.27079717590487323 0.0 2 0.0 0.0 0.0 0.7950831666747964 0.0 3 0.0 0.0 0.0 1.3070959559801174 0.0 4 0.0 0.0 0.0 1.6156568286991508 0.0 5 0.0 0.0 0.0 3.1514591735100255 0.0 6 0.0 0.0 0.0 3.4193453977854738 0.0 7 0.0 0.0 0.0 4.27162483026005 0.0 8 0.0 0.0 0.0 5.338291916523348 0.0 9 0.0 0.0 0.0 6.217241959872925 0.0 10 0.0 0.0 0.0 6.885974090957011 0.0 11 0.0 0.0 0.0 7.60450709721477 0.0 12 0.0 0.0 0.0 8.154283583334252 0.0 13 0.0 0.0 0.0 8.386294295636247 0.0 14 0.0 0.0 0.0 8.54435110167718 0.0 15 7.86743683628336E-5 0.0 0.0 9.097825283109714 0.0 16 7.86743683628336E-5 0.0 0.0 9.796768371645129 0.0 17 7.86743683628336E-5 0.0 0.0 10.680360202728112 0.0 18 7.86743683628336E-5 0.0 0.0 11.088444151426131 0.0 19 7.86743683628336E-5 0.0 0.0 11.51407248426906 0.0 20 7.86743683628336E-5 0.0 0.0 12.048507468557789 0.0 21 7.86743683628336E-5 0.0 0.0 12.561857722125279 0.0 22 7.86743683628336E-5 0.0 0.0 13.107307117984803 0.0 23 7.86743683628336E-5 0.0 0.0 13.517121902786803 0.0 24 7.86743683628336E-5 0.0 0.0 13.850779899013581 0.0 25 7.86743683628336E-5 0.0 0.0 14.191911960234828 0.0 26 7.86743683628336E-5 0.0 0.0 14.509756408420674 0.0 27 1.573487367256672E-4 0.0 0.0 14.841368871070019 0.0 28 1.573487367256672E-4 0.0 0.0 15.192885948915158 0.0 29 1.573487367256672E-4 0.0 0.0 15.619773071651894 0.0 30 1.573487367256672E-4 0.0 0.0 16.020382955355444 0.0 31 1.573487367256672E-4 0.0 0.0 16.360570924156335 0.0 32 1.573487367256672E-4 0.0 0.0 16.709098376003688 0.0 33 1.573487367256672E-4 0.0 0.0 17.055816317378696 0.0 34 1.573487367256672E-4 0.0 0.0 17.49300978237096 0.0 35 1.573487367256672E-4 0.0 0.0 17.847280463108802 0.0 36 1.573487367256672E-4 0.0 0.0 18.211070742418546 0.0 37 1.573487367256672E-4 0.0 0.0 18.565341423156383 0.0 38 1.573487367256672E-4 0.0 0.0 18.91615043168626 0.0 39 1.573487367256672E-4 0.0 0.0 19.32494244969954 0.0 40 1.573487367256672E-4 0.0 0.0 19.72051717382787 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGT 110 0.0 46.000004 3 CTATTCG 55 1.8189894E-12 46.000004 44 TGCGTTA 55 1.8189894E-12 46.000004 21 GTGCAAC 20 6.311709E-4 46.0 23 AACGTAC 20 6.311709E-4 46.0 40 TCGCACT 20 6.311709E-4 46.0 17 ACGCCCC 20 6.311709E-4 46.0 18 CGAGTGT 20 6.311709E-4 46.0 26 CGACGGA 25 3.417361E-5 46.0 15 ACGTTAC 30 1.8615592E-6 46.0 43 ACCGCGA 20 6.311709E-4 46.0 26 ATAATCG 20 6.311709E-4 46.0 34 CGGCACT 30 1.8615592E-6 46.0 5 ATTACCG 20 6.311709E-4 46.0 11 CCGGAAC 30 1.8615592E-6 46.0 20 ACTTCGA 25 3.417361E-5 46.0 14 GTGATCG 20 6.311709E-4 46.0 15 TAAGTCG 20 6.311709E-4 46.0 23 TAACGCG 40 5.6115823E-9 46.0 1 ATCCGTC 20 6.311709E-4 46.0 14 >>END_MODULE