FastQCFastQC Report
Sat 14 Jan 2017
SRR2933091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734541
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT448222.58408420440912No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC182511.0522092011661874No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC118490.6831202029816533No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC111090.6404576196238659No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC61790.3562325710375252No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT58430.3368614521075028No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC54630.3149536390318822No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC52720.30394208035439924No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT45750.26375853900253726Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT44100.2542459359565441No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT43890.25303524102341773Illumina Single End Adapter 1 (95% over 21bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC43130.24865367840829358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25110.14476452271811388No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT24780.14286200210891528Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG23930.137961570236737No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA20880.12037766763656782No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT20320.11714914781489744No Hit
GGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC19990.11524662720569881No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG19360.1116145424063196No Hit
GGACAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18130.10452332922657925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC351.0199801E-746.00000436
CGGACAT206.312348E-446.035
CGTATCA206.312348E-446.029
CGACGCC301.8619558E-646.019
ACGTTCG253.4178804E-546.012
TTGTCGT206.312348E-446.017
TAACGAC206.312348E-446.030
TACGACG206.312348E-446.02
AGCGCGA206.312348E-446.011
TTGCGAT206.312348E-446.010
TTAAACG206.312348E-446.043
ACGTATA206.312348E-446.013
ACGTACA253.4178804E-546.09
CGACTAC206.312348E-446.030
CAATCGA206.312348E-446.040
TGTCGTG301.8619558E-646.018
ACGACTA206.312348E-446.029
AGTCGAC253.4178804E-546.015
CGTTTTT101650.044.9365461
CGACGGT6000.044.8527
Produced by FastQC (version 0.11.5)