##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933090.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2022195
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53866021822821	33.0	31.0	34.0	31.0	34.0
2	32.89507243366737	34.0	31.0	34.0	31.0	34.0
3	32.97201456832798	34.0	31.0	34.0	31.0	34.0
4	36.38744334745166	37.0	37.0	37.0	35.0	37.0
5	36.36883040458512	37.0	37.0	37.0	35.0	37.0
6	36.50545471628602	37.0	37.0	37.0	35.0	37.0
7	36.560945902843194	37.0	37.0	37.0	35.0	37.0
8	36.42038329636855	37.0	37.0	37.0	35.0	37.0
9	38.05665823523449	39.0	39.0	39.0	35.0	39.0
10	37.97463350468179	39.0	38.0	39.0	35.0	39.0
11	37.62330685220763	39.0	37.0	39.0	35.0	39.0
12	37.488739216544396	39.0	37.0	39.0	35.0	39.0
13	37.34674203031854	39.0	37.0	39.0	35.0	39.0
14	38.844052131471	41.0	38.0	41.0	35.0	41.0
15	38.930504229315176	41.0	38.0	41.0	35.0	41.0
16	38.94870326551099	41.0	38.0	41.0	35.0	41.0
17	38.94834078810401	41.0	38.0	41.0	35.0	41.0
18	38.87754296692456	41.0	38.0	41.0	35.0	41.0
19	38.743839243989825	41.0	37.0	41.0	35.0	41.0
20	38.650029299844974	40.0	37.0	41.0	35.0	41.0
21	38.62445313137457	40.0	37.0	41.0	35.0	41.0
22	38.58374291302273	40.0	36.0	41.0	35.0	41.0
23	38.543762594606356	40.0	36.0	41.0	35.0	41.0
24	38.47751972485344	40.0	36.0	41.0	35.0	41.0
25	38.424165325302454	40.0	36.0	41.0	35.0	41.0
26	38.336342439774604	40.0	36.0	41.0	35.0	41.0
27	38.271278486990624	40.0	36.0	41.0	35.0	41.0
28	38.25183229114897	40.0	36.0	41.0	35.0	41.0
29	38.22068148719585	40.0	36.0	41.0	35.0	41.0
30	38.11031329817352	40.0	35.0	41.0	34.0	41.0
31	38.04697370926147	40.0	35.0	41.0	34.0	41.0
32	37.951476489656045	40.0	35.0	41.0	34.0	41.0
33	37.7842977556566	40.0	35.0	41.0	34.0	41.0
34	37.668810871355134	40.0	35.0	41.0	34.0	41.0
35	37.56328049470996	40.0	35.0	41.0	33.0	41.0
36	37.48093581479531	40.0	35.0	41.0	33.0	41.0
37	37.388855179643905	40.0	35.0	41.0	33.0	41.0
38	37.3616876710703	40.0	35.0	41.0	33.0	41.0
39	37.272943014892235	40.0	35.0	41.0	33.0	41.0
40	37.195974671087605	40.0	35.0	41.0	33.0	41.0
41	37.16333835263167	40.0	35.0	41.0	33.0	41.0
42	37.11624793850247	40.0	35.0	41.0	33.0	41.0
43	37.03999614280522	40.0	35.0	41.0	33.0	41.0
44	36.95674650565351	39.0	35.0	41.0	33.0	41.0
45	36.88689518073183	39.0	35.0	41.0	33.0	41.0
46	36.79306248902801	39.0	35.0	41.0	32.0	41.0
47	36.67517326469505	39.0	35.0	41.0	32.0	41.0
48	36.60670261770007	39.0	35.0	41.0	32.0	41.0
49	36.50421695237106	39.0	35.0	41.0	32.0	41.0
50	36.32737248386036	38.0	35.0	41.0	32.0	41.0
51	36.18987140211503	38.0	35.0	40.0	31.0	41.0
52	35.91991969122661	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	4.0
13	8.0
14	12.0
15	27.0
16	53.0
17	142.0
18	290.0
19	570.0
20	1036.0
21	1612.0
22	2683.0
23	4088.0
24	6740.0
25	10632.0
26	14758.0
27	17048.0
28	18119.0
29	19945.0
30	22535.0
31	27338.0
32	34731.0
33	47136.0
34	113241.0
35	205639.0
36	126376.0
37	152064.0
38	256411.0
39	928779.0
40	10172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.51220332361617	21.366782135254017	24.118099391997312	19.0029151491325
2	28.17082427757956	23.98784489131859	26.74242592826112	21.098904902840726
3	28.189121227181356	23.951844406696683	26.857795613182706	21.001238752939255
4	23.509651640914946	27.68204846713596	24.98364401059245	23.824655881356644
5	21.45416243240637	39.28473762421527	21.970433118467803	17.290666824910556
6	87.73956023034376	2.885824561924048	7.340686729024649	2.0339284787075433
7	89.69570194763611	1.9855157390854985	5.712505470540675	2.606276842737718
8	82.90194565805969	4.057867811956809	9.027418226234364	4.0127683037491435
9	39.519680347345336	28.064949225964853	16.32290654462107	16.092463882068742
10	31.55066647875205	24.469598629212317	24.975039499158093	19.00469539287754
11	29.240750768348256	22.061027744604253	29.798758279987837	18.899463207059654
12	22.45535173413049	27.14654125838507	31.90988010552889	18.48822690195555
13	18.50904586352948	31.626920252497904	31.76350450871454	18.100529375258073
14	17.319002371185764	28.957642561671847	36.06828223786529	17.655072829277096
15	20.267827781198154	23.054651010411952	37.30757914048843	19.369942067901462
16	20.223074431496467	24.629919468696144	28.506746382025472	26.640259717781916
17	21.815749717509934	27.585124085461587	30.18680196519129	20.412324231837186
18	24.868867740252547	30.18190629489243	26.691590079097217	18.257635885757804
19	23.232724836131034	26.909027072067726	26.752167817643702	23.106080274157538
20	23.009254794913446	27.695301392793475	29.795692304649158	19.49975150764392
21	19.882405010397118	24.935181819755268	29.25706966934445	25.925343500503168
22	17.71302965342116	30.40779944565188	28.56138997475515	23.317780926171807
23	17.685831485094166	30.732001612109617	31.437274842436064	20.144892060360153
24	23.452288231352565	27.044473950336144	28.9139276874881	20.58931013082319
25	18.267328323925238	29.32011996864793	26.495664364712603	25.91688734271423
26	17.135043850865024	29.28085570382678	30.945383605438643	22.638716839869545
27	19.15680733064813	27.296230086613804	32.85370599769063	20.693256585047436
28	18.17248089328675	30.371205546448287	28.995522192469075	22.460791367795885
29	21.987592690121378	27.380692762072893	29.965161618933884	20.666552928871845
30	25.8145727785896	28.1708737287947	26.177594148932226	19.83695934368347
31	25.192723748204305	30.312012441925727	24.269172854249963	20.22609095562001
32	23.449667316950144	28.35705755379674	23.62729608173297	24.565979047520145
33	20.602958666201825	28.78525562569386	26.52998350801975	24.081802200084564
34	20.657800063792067	29.205195344662606	26.169385247218983	23.967619344326337
35	26.84325695593155	29.610448052734778	24.12195658677823	19.424338404555446
36	21.39447481573241	32.13785020732422	24.23579328403047	22.2318816929129
37	22.514989899589306	31.931786993835903	25.682191875659864	19.87103123091492
38	20.536347879408268	29.681657802536353	24.322234008095165	25.459760309960217
39	23.851211183886818	26.68343062859912	28.933905978404656	20.531452209109407
40	19.791068616033566	26.784113302624128	29.85310516542668	23.571712915915626
41	20.46627550755491	31.233486384844188	25.58165755528028	22.71858055232062
42	20.044209386335147	27.447600256157294	27.340390021733807	25.167800335773748
43	21.953026290738528	28.005657219012015	29.366604110879514	20.674712379369943
44	21.684506192528417	29.513869829566385	25.35230281946103	23.449321158444167
45	23.92855288436575	28.161379095487828	26.320211453395935	21.589856566750488
46	23.89725026518214	27.909969117716145	27.048083889041365	21.14469672806035
47	19.896894216433132	30.864382515039352	28.255732013974917	20.9829912545526
48	20.787955662040506	28.70143581603159	27.55431597842938	22.956292543498524
49	19.948817992330117	27.625970789167216	29.99018393379471	22.435027284707953
50	20.324301068888015	27.58769554864887	29.859879981900857	22.22812340056226
51	20.175551813746942	26.09204354674005	28.29148524252112	25.440919396991884
52	20.606321348831344	25.76452814886794	28.843509157128764	24.785641345171953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2887.0
1	2852.5
2	2818.0
3	13936.0
4	25054.0
5	15267.0
6	5480.0
7	6041.0
8	6602.0
9	8428.5
10	10255.0
11	11642.0
12	13029.0
13	13437.5
14	12819.0
15	11792.0
16	10469.5
17	9147.0
18	8684.0
19	8221.0
20	8041.0
21	7861.0
22	7712.0
23	7563.0
24	8703.0
25	9843.0
26	12169.0
27	14495.0
28	17087.5
29	19680.0
30	22249.5
31	24819.0
32	27302.0
33	29785.0
34	37280.0
35	44775.0
36	50812.5
37	56850.0
38	60171.5
39	65804.5
40	68116.0
41	80072.0
42	92028.0
43	112437.0
44	132846.0
45	147135.5
46	161425.0
47	174314.0
48	187203.0
49	206562.0
50	225921.0
51	203230.5
52	180540.0
53	158200.5
54	135861.0
55	121743.5
56	107626.0
57	98973.5
58	90321.0
59	82030.0
60	73739.0
61	66828.5
62	59918.0
63	50798.0
64	34739.5
65	27801.0
66	23000.5
67	18200.0
68	14447.5
69	10695.0
70	9334.0
71	7973.0
72	6806.5
73	5640.0
74	4287.5
75	2935.0
76	2365.5
77	1796.0
78	1342.5
79	889.0
80	647.5
81	406.0
82	320.0
83	234.0
84	157.5
85	81.0
86	45.0
87	9.0
88	7.0
89	5.0
90	5.0
91	4.5
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2022195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.079279084124394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26980054371111	15.233952950148916
2	11.233825888016833	4.736019021519367
3	4.343812614680575	2.746933151819758
4	2.2080071059221837	1.8617279202185408
5	1.2812555920060182	1.350397210099494
6	0.8978207252157662	1.1355248180600457
7	0.6469550784779072	0.9546142657889185
8	0.48075686436296944	0.8107206492412456
9	0.3637483684623788	0.6900798037692056
>10	4.027071064459589	19.524171362490268
>50	1.4044497299616427	21.563857595215907
>100	0.8309392234785525	24.065628425976513
>500	0.006839976246724693	0.9676088184000728
>1k	0.0037737799981929342	1.6553558528738275
>5k	2.358612498870584E-4	0.49578641131875073
>10k+	7.075837496611752E-4	2.2076217430592147
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19433	0.9609854638153096	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14269	0.7056193888324321	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10701	0.529177453212969	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9972	0.49312751737592075	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3823	0.18905199548015894	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3561	0.17609577711348312	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCTT	3430	0.1696176679301452	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	3013	0.14899651121677188	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	2602	0.12867206179423843	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2447	0.12100712344754092	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17931010609758208	0.0
2	0.0	0.0	0.0	0.5610240357631188	0.0
3	0.0	0.0	0.0	1.006233325668395	0.0
4	0.0	0.0	0.0	1.3174298225443144	0.0
5	0.0	0.0	0.0	2.953177116944706	0.0
6	0.0	0.0	0.0	3.3705948239413113	0.0
7	0.0	0.0	0.0	4.431916803275649	0.0
8	0.0	0.0	0.0	5.7255111401224905	0.0
9	0.0	0.0	0.0	6.8498834187603075	0.0
10	0.0	0.0	0.0	7.7116697450048095	0.0
11	0.0	0.0	0.0	8.554219548559857	0.0
12	0.0	0.0	0.0	9.157474922052522	0.0
13	4.9451215139984025E-5	0.0	0.0	9.41338496040194	0.0
14	4.9451215139984025E-5	0.0	0.0	9.56772220285383	0.0
15	4.9451215139984025E-5	0.0	0.0	10.050860574771473	0.0
16	4.9451215139984025E-5	0.0	0.0	10.660742411092897	0.0
17	4.9451215139984025E-5	0.0	0.0	11.395587468073058	0.0
18	4.9451215139984025E-5	0.0	0.0	11.750202131841885	0.0
19	4.9451215139984025E-5	0.0	0.0	12.16717477790223	0.0
20	4.9451215139984025E-5	0.0	0.0	12.63186784657266	0.0
21	5.439633665398243E-4	0.0	0.0	13.116588657374784	0.0
22	5.439633665398243E-4	0.0	0.0	13.601260016961767	0.0
23	5.439633665398243E-4	0.0	0.0	13.96161102168683	0.0
24	5.439633665398243E-4	0.0	0.0	14.250307215674058	0.0
25	5.439633665398243E-4	0.0	0.0	14.532624202908226	0.0
26	5.439633665398243E-4	0.0	0.0	14.875716733549435	0.0
27	5.439633665398243E-4	0.0	0.0	15.226424751322202	0.0
28	5.439633665398243E-4	0.0	0.0	15.587913133995485	0.0
29	5.439633665398243E-4	0.0	0.0	15.930016640333895	0.0
30	5.439633665398243E-4	0.0	0.0	16.274246548923323	0.0
31	5.439633665398243E-4	0.0	0.0	16.56927249844847	0.0
32	5.439633665398243E-4	0.0	0.0	16.927052039986254	0.0
33	5.934145816798083E-4	0.0	0.0	17.283348045069836	0.0
34	5.934145816798083E-4	0.0	0.0	17.727370505811756	0.0
35	5.934145816798083E-4	0.0	0.0	18.04578688009811	0.0
36	5.934145816798083E-4	0.0	0.0	18.377505631257122	0.0
37	5.934145816798083E-4	0.0	0.0	18.805604800723966	0.0
38	5.934145816798083E-4	0.0	0.0	19.6269894841991	0.0
39	6.428657968197924E-4	0.0	0.0	20.03120371675333	0.0
40	6.428657968197924E-4	0.0	0.0	20.393137160362873	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACC	20	6.312598E-4	46.0	36
TCTACGT	40	5.6152203E-9	46.0	14
GCTCGAT	25	3.4180823E-5	46.0	32
GTGCGAC	20	6.312598E-4	46.0	40
TGTCGAT	20	6.312598E-4	46.0	23
GCGTAAT	30	1.8621104E-6	46.0	21
TATACGG	195	0.0	44.82051	1
CACGACG	565	0.0	44.37168	25
GACGGTC	565	0.0	43.964603	28
CGTTTTT	6030	0.0	43.48259	1
CTAGCGG	425	0.0	43.29412	1
GTTACGG	155	0.0	43.03226	1
ACGGTCT	580	0.0	42.827587	29
TCACGAC	600	0.0	42.55	24
CGACGGT	580	0.0	42.431038	27
CGGTCTA	580	0.0	42.431038	30
ACGGGCC	235	0.0	42.085106	4
GCGTAGG	345	0.0	42.0	1
TATGCGG	385	0.0	41.818184	1
ATAGCGG	255	0.0	41.490196	1
>>END_MODULE