FastQCFastQC Report
Sat 14 Jan 2017
SRR2933088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858785
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327121.7598592629056078No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC167910.903332015268038No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC123360.6636593258499504No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC119220.6413867122878655No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT81820.44018001006033514Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT64850.3488838138891803No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT55900.3007340816716296No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54150.29131932956205264No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC50140.2697460975852506No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG42190.22697622371602955No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC34110.18350696826152568No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC33280.17904168583241203No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC30270.16284831220393967No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC30020.16150334761685725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28170.15155060967244732No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT25910.13939212980522223No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC24930.13411986862385913No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC19100.10275529445309706No Hit
GGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18720.10071094828073178No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG206.312464E-446.0000041
TATGCGA206.312464E-446.00000439
CGGATTA405.6152203E-946.0000045
ACGTACT206.312464E-446.00000446
TAAGCGA206.312464E-446.00000438
CGGGTAG750.046.05
CTGTCGG600.046.01
CGCGGAC551.8189894E-1246.03
CGCGGAA301.8620285E-646.03
GAGTACG253.4179757E-546.043
GCACGAA501.6370905E-1146.033
TCTAGCG253.4179757E-546.09
TCGCTAA253.4179757E-546.020
TTACGAC301.8620285E-646.026
ACCGTAG253.4179757E-546.01
AGTACGG1300.046.01
ATCGCAC253.4179757E-546.028
CGACGGT9900.045.3030327
ACGTAGG2850.045.1929861
CGTTTTT73850.045.190251
Produced by FastQC (version 0.11.5)