##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1858785
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44468079955455	33.0	31.0	34.0	31.0	34.0
2	32.74652958787595	34.0	31.0	34.0	31.0	34.0
3	32.78544909712527	34.0	31.0	34.0	31.0	34.0
4	36.247856529937565	37.0	37.0	37.0	35.0	37.0
5	36.22628975379078	37.0	37.0	37.0	35.0	37.0
6	36.406156171907995	37.0	37.0	37.0	35.0	37.0
7	36.506909621069674	37.0	37.0	37.0	35.0	37.0
8	36.33623200101141	37.0	37.0	37.0	35.0	37.0
9	37.955086790564806	39.0	38.0	39.0	35.0	39.0
10	37.82949345943722	39.0	38.0	39.0	35.0	39.0
11	37.389160661399785	39.0	37.0	39.0	35.0	39.0
12	37.198167082260724	39.0	37.0	39.0	34.0	39.0
13	36.837574006676405	39.0	37.0	39.0	33.0	39.0
14	38.39307235640486	41.0	37.0	41.0	33.0	41.0
15	38.57294953423877	41.0	37.0	41.0	35.0	41.0
16	38.62820067947611	41.0	37.0	41.0	35.0	41.0
17	38.64502618646051	40.0	37.0	41.0	35.0	41.0
18	38.5433888265722	40.0	37.0	41.0	35.0	41.0
19	38.39625508060373	40.0	36.0	41.0	34.0	41.0
20	38.30262886778191	40.0	35.0	41.0	35.0	41.0
21	38.28226610393348	40.0	35.0	41.0	34.0	41.0
22	38.268082107398115	40.0	35.0	41.0	35.0	41.0
23	38.195346422528694	40.0	35.0	41.0	34.0	41.0
24	38.122655928469406	40.0	35.0	41.0	34.0	41.0
25	38.04124844992831	40.0	35.0	41.0	34.0	41.0
26	37.942276271865765	40.0	35.0	41.0	34.0	41.0
27	37.85232665423919	40.0	35.0	41.0	34.0	41.0
28	37.82332329989752	40.0	35.0	41.0	34.0	41.0
29	37.78801206164242	40.0	35.0	41.0	34.0	41.0
30	37.603918150835085	40.0	35.0	41.0	34.0	41.0
31	37.59364746326229	40.0	35.0	41.0	34.0	41.0
32	37.4988107823121	40.0	35.0	41.0	34.0	41.0
33	37.32460074726232	40.0	35.0	41.0	33.0	41.0
34	37.17994173613409	39.0	35.0	41.0	33.0	41.0
35	37.06678502355033	39.0	35.0	41.0	33.0	41.0
36	37.00730477166536	39.0	35.0	41.0	33.0	41.0
37	36.91173535400813	39.0	35.0	41.0	33.0	41.0
38	36.91465069924709	39.0	35.0	41.0	33.0	41.0
39	36.854574359057125	39.0	35.0	41.0	33.0	41.0
40	36.77588962682613	39.0	35.0	41.0	33.0	41.0
41	36.77084762358207	39.0	35.0	41.0	33.0	41.0
42	36.7568196429388	39.0	35.0	41.0	33.0	41.0
43	36.6923285909882	39.0	35.0	41.0	33.0	41.0
44	36.601282020244405	39.0	35.0	41.0	32.0	41.0
45	36.53874224291675	38.0	35.0	41.0	32.0	41.0
46	36.440043899644124	38.0	35.0	41.0	32.0	41.0
47	36.31315025675374	38.0	35.0	41.0	32.0	41.0
48	36.30538496921376	38.0	35.0	41.0	32.0	41.0
49	36.23229744160836	38.0	35.0	41.0	31.0	41.0
50	36.11702967260872	38.0	35.0	41.0	31.0	41.0
51	36.033152839085744	37.0	35.0	40.0	31.0	41.0
52	35.790699838873245	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	4.0
13	7.0
14	11.0
15	28.0
16	69.0
17	125.0
18	288.0
19	597.0
20	970.0
21	1601.0
22	2541.0
23	4184.0
24	6899.0
25	11277.0
26	15014.0
27	16882.0
28	17942.0
29	20274.0
30	24125.0
31	30022.0
32	38209.0
33	52265.0
34	130306.0
35	216805.0
36	144770.0
37	147414.0
38	216484.0
39	746549.0
40	13121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.602433847916785	22.97920415755453	25.334075753785402	18.084286240743282
2	29.625319765330577	23.59046366309175	26.220999201091033	20.563217370486637
3	28.663992877067546	23.357300602275142	27.536535963008095	20.442170557649217
4	23.16567004790764	27.20271575249424	26.12044964856075	23.51116455103737
5	21.09835188039499	37.68267981504047	22.75793058368773	18.46103772087681
6	83.16507826348932	2.5917467593078274	11.511713296588901	2.7314616806139496
7	86.24795229141617	1.619821550098586	7.713640899835108	4.41858525865014
8	76.69020354694061	3.715652966857383	13.746721648818985	5.847421837383022
9	39.695446218901054	28.08232259244614	17.26364264828907	14.958588540363731
10	32.53388638277154	20.338016500025553	28.75259914406454	18.375497973138366
11	29.674061281966445	19.64885664560452	31.23820129815982	19.43888077426921
12	22.841694978171226	26.437215708110408	34.67587698415901	16.045212329559362
13	15.878167727843726	33.038839887345766	35.220372447593455	15.862619937217055
14	13.917532151378452	31.165035224622535	37.82664482444177	17.09078779955724
15	18.45942376337231	21.96553124756225	41.145748432443774	18.42929655662166
16	18.99757099395573	26.803584061631657	27.601148061771536	26.59769688264108
17	20.8622298974868	29.277996110362416	28.987107169468228	20.872666822682557
18	22.505346234233652	34.6353128522126	26.433288411516127	16.42605250203762
19	18.352956366658866	30.25573156658785	25.692643312701573	25.698668754051706
20	19.011074438410034	30.775587278786947	30.59525442695094	19.618083855852074
21	15.499156707203898	25.743160182592394	32.194471119575425	26.56321199062829
22	12.740419144763917	32.81885747948257	28.649144468026154	25.79157890772736
23	13.835489311566427	35.41593029855524	31.55787248121757	19.190707908660766
24	24.284250195692348	26.709974526370722	27.555742057311633	21.4500332206253
25	14.584365593653919	31.217596440685718	25.594514696428046	28.603523269232323
26	14.5412729282838	29.270464308674754	33.08612884222759	23.102133920813863
27	19.17526771520106	27.641443200800524	32.45700820697391	20.726280877024507
28	15.407699115282295	33.09161629774288	27.49371229055539	24.006972296419434
29	23.737495191751602	26.789004645507685	29.500722245983262	19.97277791675745
30	25.78603765362858	25.768499315413024	29.30919928878273	19.136263742175668
31	25.311856938806805	29.02772509999812	23.695962685302497	21.964455275892586
32	23.508205628945788	26.00849479633201	27.399941359544005	23.083358215178194
33	19.650578200275987	24.940969504272953	30.487657259984346	24.920795035466718
34	16.048009855900496	26.111734278036458	31.779361249418304	26.06089461664474
35	27.546219708035085	28.42679492248969	27.0630546297716	16.963930739703624
36	19.45136204563734	29.415720484079657	30.314748612669028	20.818168857613976
37	20.187219070521873	30.872693721974297	30.055816030363918	18.884271177139905
38	16.538383944350745	27.758455119876697	28.216550058236965	27.486610877535593
39	24.50294143755195	24.74508886181027	32.40315582490713	18.348813875730652
40	17.4915872465078	23.10433966273668	36.017613656232434	23.38645943452309
41	17.922621497375975	30.913257853920705	28.432605169505887	22.731515479197434
42	18.24401423510519	23.54968433681141	32.630508638707546	25.575792789375857
43	22.457250300599586	25.448935729522244	31.6634253020118	20.430388667866374
44	20.072897080619867	28.335444927735054	26.633096350573087	24.95856164107199
45	22.582547201532183	25.561589963336267	28.643388019593445	23.212474815538105
46	24.114784657719962	25.76677776074156	30.04914500601199	20.069292575526486
47	16.724096654535085	31.14878805241058	31.17504176115043	20.952073531903906
48	19.568858151964857	25.58956522674758	29.289186215726943	25.552390405560622
49	18.403634632300133	24.723300435499535	33.457070075344916	23.41599485685542
50	17.119247250219903	26.188504856667123	33.532603286555464	23.15964460655751
51	17.268161729301667	24.079707981288852	29.549732755536546	29.10239753387293
52	17.98814817205863	22.779181024163634	33.67947341946487	25.55319738431287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3105.0
1	2867.0
2	2629.0
3	19132.0
4	35635.0
5	20032.5
6	4430.0
7	5743.0
8	7056.0
9	10158.0
10	13260.0
11	16611.0
12	19962.0
13	20649.0
14	19793.5
15	18251.0
16	16100.0
17	13949.0
18	12441.0
19	10933.0
20	9891.5
21	8850.0
22	8423.0
23	7996.0
24	8073.0
25	8150.0
26	8666.0
27	9182.0
28	10185.5
29	11189.0
30	11948.0
31	12707.0
32	15532.5
33	18358.0
34	22316.0
35	26274.0
36	29683.5
37	33093.0
38	35632.5
39	45111.0
40	52050.0
41	70956.0
42	89862.0
43	120577.0
44	151292.0
45	187288.0
46	223284.0
47	229164.5
48	235045.0
49	231416.5
50	227788.0
51	192567.5
52	157347.0
53	126596.5
54	95846.0
55	86541.5
56	77237.0
57	76119.5
58	75002.0
59	67252.0
60	59502.0
61	47289.5
62	35077.0
63	29377.0
64	18489.5
65	13302.0
66	10363.5
67	7425.0
68	5561.5
69	3698.0
70	3178.0
71	2658.0
72	2453.5
73	2249.0
74	1598.0
75	947.0
76	698.0
77	449.0
78	328.0
79	207.0
80	202.0
81	197.0
82	134.5
83	72.0
84	54.0
85	36.0
86	22.5
87	9.0
88	7.5
89	4.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1858785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.862304961662296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.14877097636001	16.261867558003832
2	12.757078727368212	6.088266060247902
3	5.694208971293376	4.076308529653077
4	3.0393406299736037	2.9010269197920375
5	1.9526669322029993	2.3297566912390764
6	1.265018494200289	1.8111754254450072
7	0.8693628373593107	1.4521500803182728
8	0.6535039347316206	1.2475288147369747
9	0.48234514105886717	1.0358880167450437
>10	3.8279898300052135	19.331645212631493
>50	0.7806345616001149	13.240878367186525
>100	0.503690430773048	18.685172015672006
>500	0.01428104985375268	2.2542785882030807
>1k	0.009067333240477891	3.6343244099385377
>5k	0.0011334166550597364	1.659863053571679
>10k+	9.067333240477891E-4	3.9898702566154154
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32712	1.7598592629056078	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	16791	0.903332015268038	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	12336	0.6636593258499504	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	11922	0.6413867122878655	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	8182	0.44018001006033514	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	6485	0.3488838138891803	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	5590	0.3007340816716296	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5415	0.29131932956205264	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5014	0.2697460975852506	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG	4219	0.22697622371602955	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3411	0.18350696826152568	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3328	0.17904168583241203	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	3027	0.16284831220393967	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3002	0.16150334761685725	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2817	0.15155060967244732	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	2591	0.13939212980522223	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2493	0.13411986862385913	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC	1910	0.10275529445309706	No Hit
GGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1872	0.10071094828073178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4558891964374578	0.0
2	0.0	0.0	0.0	1.3668068119766406	0.0
3	0.0	0.0	0.0	2.558068846047284	0.0
4	0.0	0.0	0.0	3.362680460623472	0.0
5	0.0	0.0	0.0	7.2675430455916095	0.0
6	0.0	0.0	0.0	8.13730474476607	0.0
7	0.0	0.0	0.0	10.308023789733616	0.0
8	0.0	0.0	0.0	13.255163991532102	0.0
9	0.0	0.0	0.0	15.208321564893197	0.0
10	0.0	0.0	0.0	16.895929330180735	0.0
11	0.0	0.0	0.0	18.674779493055947	0.0
12	0.0	0.0	0.0	19.869484636469522	0.0
13	0.0	0.0	0.0	20.404081160543043	0.0
14	0.0	0.0	0.0	20.731122749538006	0.0
15	0.0	0.0	0.0	21.63257181438413	0.0
16	0.0	0.0	0.0	22.893556812649123	0.0
17	0.0	0.0	0.0	24.27553482516805	0.0
18	0.0	0.0	0.0	25.027154835013196	0.0
19	0.0	0.0	0.0	25.729979529638985	0.0
20	0.0	0.0	0.0	26.602861546655475	0.0
21	0.0	0.0	0.0	27.464392062556993	0.0
22	0.0	0.0	0.0	28.30720067140632	0.0
23	0.0	0.0	0.0	28.903073782067317	0.0
24	5.3798583483296885E-5	0.0	0.0	29.365257412772323	0.0
25	5.3798583483296885E-5	0.0	0.0	29.84250464685265	0.0
26	5.3798583483296885E-5	0.0	0.0	30.446232350702207	0.0
27	5.3798583483296885E-5	0.0	0.0	30.989490446716538	0.0
28	5.3798583483296885E-5	0.0	0.0	31.616082548546498	0.0
29	5.3798583483296885E-5	0.0	0.0	32.12867545197535	0.0
30	5.3798583483296885E-5	0.0	0.0	32.7185769198697	0.0
31	5.3798583483296885E-5	0.0	0.0	33.14934217782046	0.0
32	5.3798583483296885E-5	0.0	0.0	33.64794744954365	0.0
33	5.3798583483296885E-5	0.0	0.0	34.22407648006628	0.0
34	5.3798583483296885E-5	0.0	0.0	34.89467582318557	0.0
35	5.3798583483296885E-5	0.0	0.0	35.34152685759784	0.0
36	5.3798583483296885E-5	0.0	0.0	35.82275518685593	0.0
37	5.3798583483296885E-5	0.0	0.0	36.465702058064814	0.0
38	5.3798583483296885E-5	0.0	0.0	37.8861460577743	0.0
39	5.3798583483296885E-5	0.0	0.0	38.44651210333632	0.0
40	5.3798583483296885E-5	0.0	0.0	38.939414725210284	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGCG	20	6.312464E-4	46.000004	1
TATGCGA	20	6.312464E-4	46.000004	39
CGGATTA	40	5.6152203E-9	46.000004	5
ACGTACT	20	6.312464E-4	46.000004	46
TAAGCGA	20	6.312464E-4	46.000004	38
CGGGTAG	75	0.0	46.0	5
CTGTCGG	60	0.0	46.0	1
CGCGGAC	55	1.8189894E-12	46.0	3
CGCGGAA	30	1.8620285E-6	46.0	3
GAGTACG	25	3.4179757E-5	46.0	43
GCACGAA	50	1.6370905E-11	46.0	33
TCTAGCG	25	3.4179757E-5	46.0	9
TCGCTAA	25	3.4179757E-5	46.0	20
TTACGAC	30	1.8620285E-6	46.0	26
ACCGTAG	25	3.4179757E-5	46.0	1
AGTACGG	130	0.0	46.0	1
ATCGCAC	25	3.4179757E-5	46.0	28
CGACGGT	990	0.0	45.30303	27
ACGTAGG	285	0.0	45.192986	1
CGTTTTT	7385	0.0	45.19025	1
>>END_MODULE