FastQCFastQC Report
Sat 14 Jan 2017
SRR2933087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750087
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC160892.144951185662463No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111921.4920935838109446No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG74230.9896185375829737No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT30920.4122188492801502TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT26810.3574252053428469TruSeq Adapter, Index 27 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT26080.34769300094522365TruSeq Adapter, Index 27 (96% over 26bp)
GGGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT21840.2911662247179327TruSeq Adapter, Index 27 (95% over 23bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCG20370.2715684980542257No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA19350.25797007547124534No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG16020.21357522527386824No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCACCTTTCT14490.1931775913993977No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC14360.19144445910941TruSeq Adapter, Index 27 (100% over 24bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG12240.1631810709957645No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG11450.15264895938737771No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC9310.12411893553681105No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG9190.12251912111528396No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT8460.11278691671766075TruSeq Adapter, Index 27 (96% over 26bp)
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8280.1103871950853701No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.10278807658311635No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCCACC7630.10172153363543163No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCC7550.1006549906877469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTG351.0189251E-746.00000413
CGGACAT301.8605315E-646.010
CAGCGTA301.8605315E-646.036
AAGTACG405.6061253E-946.01
AACCGGT253.4160126E-546.030
ACCGGTT253.4160126E-546.031
CCCCTAT206.310045E-446.031
GATCGTA206.310045E-446.027
CACGGTT206.310045E-446.011
ATAGGCG206.310045E-446.011
CCGATTA253.4160126E-546.012
CAACGAA206.310045E-446.019
TTAGCGT206.310045E-446.041
ACGTTAG301.8605315E-646.01
CGACCAA900.046.029
CGATTAG253.4160126E-546.013
TACGGGT600.046.04
TATGCGT206.310045E-446.011
ACGGGTG253.4160126E-546.05
GCCGCTA206.310045E-446.018
Produced by FastQC (version 0.11.5)