##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	750087
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.351103272020445	33.0	31.0	34.0	31.0	34.0
2	32.71860997457628	34.0	31.0	34.0	31.0	34.0
3	32.745792154776716	34.0	31.0	34.0	31.0	34.0
4	36.229002769012126	37.0	37.0	37.0	35.0	37.0
5	36.04551605347113	37.0	35.0	37.0	35.0	37.0
6	36.15023990550429	37.0	35.0	37.0	35.0	37.0
7	36.42874226589716	37.0	37.0	37.0	35.0	37.0
8	36.35828377241573	37.0	37.0	37.0	35.0	37.0
9	38.17174007815093	39.0	39.0	39.0	37.0	39.0
10	37.8662501816456	39.0	38.0	39.0	35.0	39.0
11	37.54232908982558	39.0	37.0	39.0	35.0	39.0
12	37.10643831982157	39.0	35.0	39.0	35.0	39.0
13	36.99141166291377	39.0	35.0	39.0	33.0	39.0
14	37.67528166732659	40.0	36.0	41.0	32.0	41.0
15	37.9257286154806	40.0	35.0	41.0	33.0	41.0
16	38.19340289859709	40.0	35.0	41.0	33.0	41.0
17	38.276467929720155	40.0	35.0	41.0	34.0	41.0
18	38.19605992371552	40.0	36.0	41.0	34.0	41.0
19	38.15191837746821	40.0	36.0	41.0	34.0	41.0
20	37.96307894950852	40.0	35.0	41.0	33.0	41.0
21	37.924675404319764	40.0	35.0	41.0	34.0	41.0
22	37.86673679186548	40.0	35.0	41.0	34.0	41.0
23	37.857912482152074	40.0	35.0	41.0	34.0	41.0
24	37.82282855188798	40.0	35.0	41.0	34.0	41.0
25	37.65284027052862	39.0	35.0	41.0	34.0	41.0
26	37.569203305749866	39.0	35.0	41.0	34.0	41.0
27	37.47321044092219	39.0	35.0	41.0	34.0	41.0
28	37.470845381935696	39.0	35.0	41.0	34.0	41.0
29	37.432081878502096	39.0	35.0	41.0	34.0	41.0
30	37.38592989879841	39.0	35.0	41.0	33.0	41.0
31	37.2626162031871	40.0	35.0	41.0	33.0	41.0
32	37.100433683026104	39.0	35.0	41.0	33.0	41.0
33	37.00664989527881	39.0	35.0	41.0	33.0	41.0
34	36.87196418548782	39.0	35.0	41.0	32.0	41.0
35	36.77360892803102	39.0	35.0	41.0	32.0	41.0
36	36.73942356020035	39.0	35.0	41.0	32.0	41.0
37	36.641185622467795	39.0	35.0	41.0	32.0	41.0
38	36.62281308701524	39.0	35.0	41.0	32.0	41.0
39	36.589598273266965	39.0	35.0	41.0	32.0	41.0
40	36.493616073868765	39.0	35.0	41.0	31.0	41.0
41	36.33359730271289	39.0	35.0	41.0	31.0	41.0
42	36.36292990013158	39.0	35.0	41.0	31.0	41.0
43	36.31760182485498	39.0	35.0	41.0	31.0	41.0
44	36.274969436878656	39.0	35.0	41.0	31.0	41.0
45	36.21039826046845	38.0	35.0	41.0	31.0	41.0
46	36.156784479667024	38.0	35.0	41.0	31.0	41.0
47	36.047537152356995	38.0	35.0	41.0	31.0	41.0
48	35.95024043877577	38.0	35.0	41.0	31.0	41.0
49	35.95409865788902	38.0	35.0	41.0	31.0	41.0
50	35.83319401616079	38.0	35.0	41.0	30.0	41.0
51	35.78162266510418	38.0	35.0	41.0	30.0	41.0
52	35.532359579622096	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	3.0
13	6.0
14	7.0
15	17.0
16	63.0
17	102.0
18	196.0
19	345.0
20	603.0
21	937.0
22	1460.0
23	2241.0
24	3882.0
25	5908.0
26	7322.0
27	8312.0
28	8856.0
29	9731.0
30	11253.0
31	13899.0
32	17250.0
33	24105.0
34	62097.0
35	87568.0
36	47666.0
37	52041.0
38	80958.0
39	300730.0
40	2526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.634602386123206	22.317944451776928	23.814570843115533	14.23288231898433
2	39.67739742189906	21.967851729199413	24.69700181445619	13.657749034445338
3	34.65917953517392	22.17009493565413	29.303800759111944	13.866924770060008
4	31.218911939548345	25.819271631157452	28.62147990833063	14.340336520963568
5	24.786324786324787	28.69920422564316	30.833889935434154	15.6805810525979
6	27.334162570475158	33.509046283964395	29.32166535348566	9.835125792074786
7	82.36431240642752	2.2132099343142864	11.646249035111927	3.7762286241462655
8	79.34012987826745	2.2886678478629814	12.697327110055234	5.673875163814331
9	73.32322783890403	4.03633178551288	15.616721793605276	7.023718581977824
10	48.98125150815839	17.663151074475362	20.748259868521917	12.607337548844333
11	32.26479061762169	23.294231202513842	27.986753536589752	16.454224643274713
12	31.16198520971567	16.931502612363634	33.94806202480512	17.95845015311557
13	28.927311098579235	19.38161839893239	37.030771097219386	14.66029940526899
14	17.538632185333167	22.29154751382173	40.65715043721595	19.512669863629153
15	14.006108624732864	28.538289558411222	40.16600740980713	17.289594407048785
16	27.472813153674174	25.26253621246602	31.660860673495208	15.603789960364598
17	23.891228617480373	23.99361674045811	27.68092234634116	24.434232295720363
18	20.610942464007508	24.29384858023136	36.66228050879432	18.432928446966816
19	21.546700582732402	25.558635198317	29.06182882785597	23.832835391094633
20	16.504218843947434	38.18277079858736	26.741831280904748	18.57117907656045
21	22.776557919281363	27.410153755497696	33.84980675574967	15.963481569471275
22	16.993095467592426	24.487292807367677	36.620152062360766	21.89945966267913
23	18.855146136381514	24.80578919512003	35.6853271687151	20.653737499783357
24	23.770309310786615	26.844752675356325	29.509376912278174	19.875561101578885
25	23.16317973781708	24.442764639301842	30.914013974378975	21.48004164850211
26	16.87737555776863	28.806125156148553	27.8577018399199	26.45879744616291
27	21.94638755237726	24.75606163018423	32.06828007951078	21.229270737927735
28	15.70551149400003	29.286469436212066	26.52318997662938	28.484829093158524
29	19.79850337360866	28.865851561218896	25.65889023539936	25.67675482977308
30	24.10013771735812	25.02096423481543	25.860866806117155	25.018031241709295
31	20.695999264085366	25.576766428427632	29.709886986442907	24.01734732104409
32	23.55713403911813	23.38208767782937	24.678337312871708	28.382440970180795
33	22.411400277567804	24.110136557492666	30.069711913418047	23.40875125152149
34	16.051204726918343	21.53643510686094	36.768801485694325	25.64355868052639
35	17.161209299721232	26.745430863353185	31.00666989295908	25.0866899439665
36	20.075004632795928	24.899111703042447	29.104090592157977	25.921793072003645
37	15.828163932983774	28.57508529010635	29.69802169614991	25.898729080759963
38	19.15657783697091	24.403035914500585	32.28012217249466	24.160264076033847
39	18.290278327713985	26.047111868356605	28.896114717359456	26.766495086569957
40	24.5058239910837	21.952120220721064	32.76673239237582	20.775323395819417
41	16.724193326907415	23.627792509402244	28.889715459673344	30.758298704017
42	16.567411513597758	26.11577057061381	31.550340160541378	25.76647775524706
43	17.695947270116665	24.738863625152817	32.08787780617449	25.47731129855603
44	22.15889623470344	22.641106964925402	27.659058215913625	27.540938584457535
45	18.334139906437517	22.628575085290105	27.605864386397844	31.43142062187453
46	22.593779121621893	22.421665753439267	29.71281997954904	25.2717351453898
47	16.60020770923906	23.000265302558237	32.811527196178574	27.587999792024124
48	15.359418307476332	26.81422288347885	31.853638311289224	25.972720497755592
49	23.5152722284215	21.794938453806026	31.233843540815933	23.45594577695654
50	19.78063877923494	21.899326344810667	31.304368693231588	27.015666182722804
51	18.126030713770536	22.367272063107347	28.917045622707764	30.589651600414353
52	17.84566323639791	23.141048971652623	32.22452862134659	26.788759170602876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	1533.5
2	2641.0
3	8166.5
4	13692.0
5	8027.5
6	2363.0
7	2566.0
8	2769.0
9	3573.5
10	4378.0
11	5447.0
12	6516.0
13	6926.0
14	6811.5
15	6287.0
16	5895.0
17	5503.0
18	5077.5
19	4652.0
20	4327.5
21	4003.0
22	3711.5
23	3420.0
24	3482.5
25	3545.0
26	4123.5
27	4702.0
28	5041.5
29	5381.0
30	6212.5
31	7044.0
32	8047.5
33	9051.0
34	10144.0
35	11237.0
36	11939.0
37	12641.0
38	13221.0
39	16025.5
40	18250.0
41	22226.5
42	26203.0
43	30220.0
44	34237.0
45	43082.5
46	51928.0
47	64249.0
48	76570.0
49	85201.5
50	93833.0
51	83485.0
52	73137.0
53	64933.5
54	56730.0
55	52028.5
56	47327.0
57	41911.0
58	36495.0
59	40774.0
60	45053.0
61	33218.5
62	21384.0
63	17137.5
64	11006.5
65	9122.0
66	7356.5
67	5591.0
68	4725.0
69	3859.0
70	3036.5
71	2214.0
72	1801.5
73	1389.0
74	1177.5
75	966.0
76	889.5
77	813.0
78	543.5
79	274.0
80	229.0
81	184.0
82	150.0
83	116.0
84	84.0
85	52.0
86	31.5
87	11.0
88	32.0
89	33.5
90	14.0
91	8.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	750087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.015069033666244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.89569569827492	18.183413487467504
2	12.457096893876434	6.4814447130652955
3	5.117603629322611	3.9940443511130583
4	2.581219365747371	2.686023999638162
5	1.4923652153906932	1.9411992050915534
6	0.9562656474688979	1.4926390100056153
7	0.692758563250226	1.261551329263265
8	0.5016893665469329	1.0441186803339797
9	0.38993322146752263	0.9129725707497722
>10	4.69614504282572	29.436035361990086
>50	1.110211666484323	18.791377525356946
>100	0.09153204105328219	4.374608180141265
>500	0.010284498994750807	1.8961406790410782
>1k	0.005656474447112944	2.8618694473490747
>5k	5.142249497375403E-4	0.9930190673431581
>10k+	0.0010284498994750806	3.649542392050208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	16089	2.144951185662463	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11192	1.4920935838109446	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	7423	0.9896185375829737	No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	3092	0.4122188492801502	TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2681	0.3574252053428469	TruSeq Adapter, Index 27 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2608	0.34769300094522365	TruSeq Adapter, Index 27 (96% over 26bp)
GGGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2184	0.2911662247179327	TruSeq Adapter, Index 27 (95% over 23bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCG	2037	0.2715684980542257	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1935	0.25797007547124534	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	1602	0.21357522527386824	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCACCTTTCT	1449	0.1931775913993977	No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1436	0.19144445910941	TruSeq Adapter, Index 27 (100% over 24bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1224	0.1631810709957645	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG	1145	0.15264895938737771	No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	931	0.12411893553681105	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	919	0.12251912111528396	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	846	0.11278691671766075	TruSeq Adapter, Index 27 (96% over 26bp)
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	828	0.1103871950853701	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.10278807658311635	No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCCACC	763	0.10172153363543163	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCC	755	0.1006549906877469	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4247507289154458	0.0
2	0.0	0.0	0.0	1.365841562378764	0.0
3	0.0	0.0	0.0	2.15095048974319	0.0
4	0.0	0.0	0.0	3.050312830378343	0.0
5	0.0	0.0	0.0	5.71866996761709	0.0
6	0.0	0.0	0.0	7.735635999557385	0.0
7	0.0	0.0	0.0	9.23706183416057	0.0
8	0.0	0.0	0.0	11.999408068664035	0.0
9	0.0	0.0	0.0	13.2620616008543	0.0
10	0.0	0.0	0.0	14.8202808474217	0.0
11	0.0	0.0	0.0	17.326523456612367	0.0
12	0.0	0.0	0.0	18.882476299415934	0.0
13	0.0	0.0	0.0	19.560397660538044	0.0
14	0.0	0.0	0.0	19.85649664638902	0.0
15	0.0	0.0	0.0	20.3156433853673	0.0
16	0.0	0.0	0.0	21.137148090821466	0.0
17	0.0	0.0	0.0	22.001581149919943	0.0
18	0.0	0.0	0.0	23.008664328271255	0.0
19	0.0	0.0	0.0	23.585930698705617	0.0
20	0.0	0.0	0.0	24.368373268700832	0.0
21	0.0	0.0	0.0	25.04829439784985	0.0
22	0.0	0.0	0.0	25.75114620037409	0.0
23	0.0	0.0	0.0	26.371740878058144	0.0
24	2.6663573692118384E-4	0.0	0.0	26.832887385063334	0.0
25	2.6663573692118384E-4	0.0	0.0	27.403087908469285	0.0
26	2.6663573692118384E-4	0.0	0.0	27.853568985997626	0.0
27	2.6663573692118384E-4	0.0	0.0	28.49069507937079	0.0
28	2.6663573692118384E-4	0.0	0.0	28.990903721834933	0.0
29	2.6663573692118384E-4	0.0	0.0	29.585768050906093	0.0
30	2.6663573692118384E-4	0.0	0.0	31.025201076675106	0.0
31	2.6663573692118384E-4	0.0	0.0	31.56860470852048	0.0
32	2.6663573692118384E-4	0.0	0.0	31.961492466873842	0.0
33	2.6663573692118384E-4	0.0	0.0	32.46743377768179	0.0
34	2.6663573692118384E-4	0.0	0.0	33.012037270343306	0.0
35	2.6663573692118384E-4	0.0	0.0	33.536109811261895	0.0
36	2.6663573692118384E-4	0.0	0.0	33.88766903039247	0.0
37	2.6663573692118384E-4	0.0	0.0	34.358947695400666	0.0
38	2.6663573692118384E-4	0.0	0.0	34.90675081690524	0.0
39	2.6663573692118384E-4	0.0	0.0	36.415109180668374	0.0
40	2.6663573692118384E-4	0.0	0.0	36.863323854432885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTG	35	1.0189251E-7	46.000004	13
CGGACAT	30	1.8605315E-6	46.0	10
CAGCGTA	30	1.8605315E-6	46.0	36
AAGTACG	40	5.6061253E-9	46.0	1
AACCGGT	25	3.4160126E-5	46.0	30
ACCGGTT	25	3.4160126E-5	46.0	31
CCCCTAT	20	6.310045E-4	46.0	31
GATCGTA	20	6.310045E-4	46.0	27
CACGGTT	20	6.310045E-4	46.0	11
ATAGGCG	20	6.310045E-4	46.0	11
CCGATTA	25	3.4160126E-5	46.0	12
CAACGAA	20	6.310045E-4	46.0	19
TTAGCGT	20	6.310045E-4	46.0	41
ACGTTAG	30	1.8605315E-6	46.0	1
CGACCAA	90	0.0	46.0	29
CGATTAG	25	3.4160126E-5	46.0	13
TACGGGT	60	0.0	46.0	4
TATGCGT	20	6.310045E-4	46.0	11
ACGGGTG	25	3.4160126E-5	46.0	5
GCCGCTA	20	6.310045E-4	46.0	18
>>END_MODULE