FastQCFastQC Report
Sat 14 Jan 2017
SRR2933086.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933086.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506896
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313256.179768631040687No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC23370.4610413181402102No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG21670.42750386667087525No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT19780.3902181118020264No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT18810.3710820365518765No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC17240.3401092137243143Illumina Single End Adapter 1 (95% over 21bp)
GCTGAGGCTGACATAACTCTTTTCATGTTTAATTCTTGTCCCTTTAGTAGGG16060.31683027682207No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT14090.27796628894289954No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT13300.2623812379659733Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT12500.24659890786275684Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC11030.21759887629809663No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC10830.21365329377229253No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC10470.20655124522584514No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGT9150.18051040055553802No Hit
GCCATGGATGTTATGGTCAGCTTCGGATTGAAGTCGAGTCAAATTTCAAAAC8230.16236072093683912No Hit
CGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG7760.15308860200119948No Hit
CACCGGCTTCAGCTCTACAGCCAGAAACAGCTGGAATGGGTCACTCAAATCC7420.14638111170733248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6950.1371089927716928No Hit
GCCAGATAAGACCTCTTCAGCTCCTTCAGTCCTTCCCCTAATTCCTCCATTG5120.1010069126605852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG351.018052E-746.0000042
TTGCGAG700.046.0000041
GTTTGCG206.3081004E-446.01
GGTACGG301.8593291E-646.02
GGCCGAT206.3081004E-446.08
CCAGCGA453.092282E-1046.045
TCGGCCC551.8189894E-1246.08
CGTTATA301.8593291E-646.011
ATCGTAG253.414435E-546.01
GTAGCGG453.092282E-1046.02
AATTGCG206.3081004E-446.01
CGCTAGG301.8593291E-646.02
CGGGATA850.046.06
GACGGGC253.414435E-546.04
CGTACCC253.414435E-546.037
CCGGTAT206.3081004E-446.042
TGCGTAC253.414435E-546.035
CGTTTTT73050.044.8350451
TTCGGAT1350.044.29629522
TCGGATT1300.044.2307723
Produced by FastQC (version 0.11.5)